Batch fermentation of xylose for xylitol production in stirred tank bioreactor

Very high gravity (VHG) ethanolic fermentation is a promising technology used for producing bioethanol. However, the technology is often associated with the excessive amount of glucose that is entirely supplied in the beginning of the culture causing the fermentation process to be sluggish and therefore inhibits complete utilisation of glucose. The high concentration of glucose in the fermentation medium also elevates the osmotic pressure, which has a destructive effect on yeast cells. This study aims to enhance the production of VHG bioethanol from sago hampas hydrolysate (SHH) via fed-batch fermentation. The fermentations were performed in a 2-L stirred tank bioreactor. Batch fermentation was conducted as a control. Our results showed that the maximum yeast cell concentration achieved was significantly improved by 1.5-fold when the fermentation was carried out in fed-batch mode. The ethanol yield attained in the fed-batch culture represents an enhancement of 22% over that achieved in the batch culture. Moreover, the ethanol productivity achieved in the fed-batch culture was found to be increased by 1.8 times in comparison to the productivity attained in the batch culture. In general, this work provides useful insights into promising techniques for enhancing VHG fermentations in the stirred tank bioreactor employing agricultural residues as feedstocks.

Download Full-text

Dynamical simulation of a continuous stirred tank bioreactor with the use of cellular automata for the biofilm description

Chemical Engineering Research and Design ◽

10.1016/j.cherd.2021.02.019 ◽

2021 ◽

Vol 168 ◽

pp. 264-274

Author(s):

Szymon Skoneczny ◽

Monika Cioch-Skoneczny

Keyword(s):

Cellular Automata ◽

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Dynamical Simulation ◽

Continuous Stirred Tank

Download Full-text

High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

Process Biochemistry ◽

10.1016/j.procbio.2018.03.005 ◽

2018 ◽

Vol 69 ◽

pp. 1-11 ◽

Cited By ~ 15

Author(s):

Willian Daniel Hahn Schneider ◽

Roselei Claudete Fontana ◽

Simone Mendonça ◽

Félix Gonçalves de Siqueira ◽

Aldo José Pinheiro Dillon ◽

...

Keyword(s):

Nitrogen Sources ◽

White Rot ◽

Stirred Tank ◽

Carbon And Nitrogen Sources ◽

Stirred Tank Bioreactor ◽

Carbon And Nitrogen ◽

High Level ◽

Level Production

Download Full-text

Utilisation of winery wastewater for xanthan production in stirred tank bioreactor: Bioprocess modelling and optimisation

Food and Bioproducts Processing ◽

10.1016/j.fbp.2019.06.019 ◽

2019 ◽

Vol 117 ◽

pp. 113-125 ◽

Cited By ~ 3

Author(s):

Zorana Rončević ◽

Jovana Grahovac ◽

Siniša Dodić ◽

Damjan Vučurović ◽

Jelena Dodić

Keyword(s):

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Winery Wastewater ◽

Xanthan Production

Download Full-text

Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/586363 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Mohd Azmir Arifin ◽

Maizirwan Mel ◽

Mohamed Ismail Abdul Karim ◽

Aini Ideris

Keyword(s):

Cell Culture ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

High Speed ◽

Virus Titer ◽

Vero Cells ◽

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

Download Full-text