The Polymerase Chain Reaction (PCR) in the routine genetic characterization of retinoblastoma: A tool for the clinical laboratory

1997 ◽  
Vol 41 (4) ◽  
pp. 331-340 ◽  
Author(s):  
Domenico Mastrangelo ◽  
Nicola Squitieri ◽  
Stefano Bruni ◽  
Theodora Hadjistilianou ◽  
Renato Frezzotti
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Characterization ◽  
Clinical Laboratory ◽  
Chain Reaction ◽  
Polymerase Chain

Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting

1998 ◽  
Vol 84 (9) ◽  
pp. 707-714 ◽  
Author(s):  
Wieger L. Homan ◽  
Margriet Gilsing ◽  
Hafida Bentala ◽  
Louis Limper ◽  
Frans van Knapen
Keyword(s):  
Polymerase Chain Reaction ◽  
Giardia Duodenalis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Rhodococcus equi Infections in Goats: Characterization of Virulence Plasmids

2017 ◽  
Vol 55 (2) ◽  
pp. 273-276 ◽  
Author(s):  
Lauren W. Stranahan ◽  
Quinci D. Plumlee ◽  
Sara D. Lawhon ◽  
Noah D. Cohen ◽  
Laura K. Bryan
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Nodes ◽  
Rhodococcus Equi ◽  
Caseous Lymphadenitis ◽  
Chain Reaction ◽  
Disseminated Infection ◽  
Virulence Plasmids ◽  
Visceral Organs ◽  
Polymerase Chain

Rhodococcus equi is an uncommon cause of systemic pyogranulomatous infections in goats with macroscopic similarities to caseous lymphadenitis caused by Corynebacterium pseudotuberculosis. Caprine cases have previously been reported to be caused by avirulent R. equi strains. Six cases of R. equi infection in goats yielding 8 R. equi isolates were identified from 2000 to 2017. Lesions varied from bronchopneumonia, vertebral and humeral osteomyelitis, and subcutaneous abscesses, to disseminated infection involving the lungs, lymph nodes, and multiple visceral organs. Isolates of R. equi from infected goats were analyzed by polymerase chain reaction for R. equi virulence-associated plasmid ( vap) genes. Seven of 8 isolates carried the VapN plasmid, originally characterized in bovine isolates, while 1 isolate lacked virulence plasmids and was classified as avirulent. The VapN plasmid has not been described in isolates cultured from goats.

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