Rhodococcus equi Infections in Goats: Characterization of Virulence Plasmids

Rhodococcus equi is an uncommon cause of systemic pyogranulomatous infections in goats with macroscopic similarities to caseous lymphadenitis caused by Corynebacterium pseudotuberculosis. Caprine cases have previously been reported to be caused by avirulent R. equi strains. Six cases of R. equi infection in goats yielding 8 R. equi isolates were identified from 2000 to 2017. Lesions varied from bronchopneumonia, vertebral and humeral osteomyelitis, and subcutaneous abscesses, to disseminated infection involving the lungs, lymph nodes, and multiple visceral organs. Isolates of R. equi from infected goats were analyzed by polymerase chain reaction for R. equi virulence-associated plasmid ( vap) genes. Seven of 8 isolates carried the VapN plasmid, originally characterized in bovine isolates, while 1 isolate lacked virulence plasmids and was classified as avirulent. The VapN plasmid has not been described in isolates cultured from goats.

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740: Significance of Micrometastases in Pelvic Lymph Nodes Detected by Real-Time Reverse Transcriptase Polymerase Chain Reaction in Patients with Clinically Localized Prostate Cancer Undergoing Radical Prostatectomy Following Neoadjuvant Hormonal Therapy

The Journal of Urology ◽

10.1016/s0022-5347(18)30980-7 ◽

2007 ◽

Vol 177 (4S) ◽

pp. 248-248

Author(s):

Hideaki Miyake ◽

Toshifumi Kurahashi ◽

Atsushi Takenaka ◽

Isao Hara ◽

Masato Fujisawa

Keyword(s):

Prostate Cancer ◽

Polymerase Chain Reaction ◽

Radical Prostatectomy ◽

Lymph Nodes ◽

Reverse Transcriptase ◽

Real Time ◽

Hormonal Therapy ◽

Chain Reaction ◽

Pelvic Lymph Nodes ◽

Polymerase Chain

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Characterization of a new human apolipoprotein A-I Yame by direct sequencing of polymerase chain reaction-amplified DNA.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)41957-1 ◽

1991 ◽

Vol 32 (8) ◽

pp. 1275-1280

Author(s):

Y Takada ◽

J Sasaki ◽

M Seki ◽

S Ogata ◽

Y Teranishi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Direct Sequencing ◽

Chain Reaction ◽

Human Apolipoprotein ◽

Apolipoprotein A ◽

Polymerase Chain

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Prognostic Significance of Cytokeratin-20 Reverse Transcriptase Polymerase Chain Reaction in Lymph Nodes of Node-Negative Colorectal Cancer Patients

Journal of Clinical Oncology ◽

10.1200/jco.20.4.1049 ◽

2002 ◽

Vol 20 (4) ◽

pp. 1049-1055 ◽

Cited By ~ 45

Author(s):

R. Rosenberg

Keyword(s):

Colorectal Cancer ◽

Polymerase Chain Reaction ◽

Lymph Nodes ◽

Cancer Patients ◽

Prognostic Significance ◽

Cytokeratin 20 ◽

Chain Reaction ◽

Node Negative ◽

Polymerase Chain ◽

Colorectal Cancer Patients

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Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting

Parasitology Research ◽

10.1007/s004360050474 ◽

1998 ◽

Vol 84 (9) ◽

pp. 707-714 ◽

Cited By ~ 58

Author(s):

Wieger L. Homan ◽

Margriet Gilsing ◽

Hafida Bentala ◽

Louis Limper ◽

Frans van Knapen

Keyword(s):

Polymerase Chain Reaction ◽

Giardia Duodenalis ◽

Chain Reaction ◽

Polymerase Chain

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Frequent detection of human herpesvirus-6 sequences by polymerase chain reaction in paraffin-embedded lymph nodes from patients with angioimmunoblastic lymphadenopathy and angioimmunoblastic lymphadenopathy-like lymphoma

Leukemia Research ◽

10.1016/0145-2126(93)90049-q ◽

1993 ◽

Vol 17 (11) ◽

pp. 1003-1011 ◽

Cited By ~ 37

Author(s):

Mario Luppi ◽

Roberto Marasca ◽

Patrizia Barozzi ◽

Tullio Artusi ◽

Giuseppe Torelli

Keyword(s):

Polymerase Chain Reaction ◽

Lymph Nodes ◽

Human Herpesvirus ◽

Human Herpesvirus 6 ◽

Chain Reaction ◽

Angioimmunoblastic Lymphadenopathy ◽

Polymerase Chain ◽

Herpesvirus 6

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Characterization of chloramphenicol acetyltransferase gene by multiplex polymerase chain reaction in multidrug-resistant strains isolated from aquatic environments

Aquaculture ◽

10.1016/s0044-8486(02)00169-2 ◽

2003 ◽

Vol 217 (1-4) ◽

pp. 11-21 ◽

Cited By ~ 31

Author(s):

Min Ho Yoo ◽

Min-Do Huh ◽

Eun-heui Kim ◽

Hyoung-Ho Lee ◽

Hyun Do Jeong

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Multidrug Resistant ◽

Chloramphenicol Acetyltransferase ◽

Aquatic Environments ◽

Chain Reaction ◽

Resistant Strains ◽

Polymerase Chain ◽

Chloramphenicol Acetyltransferase Gene

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Promoter Hypermethylation for Molecular Nodal Staging in Non–Small Cell Lung Cancer

Archives of Pathology & Laboratory Medicine ◽

10.5858/2007-131-936-phfmns ◽

2007 ◽

Vol 131 (6) ◽

pp. 936-941

Author(s):

A. Mazin Safar ◽

Horace Spencer ◽

Xiaobo Su ◽

Craig A. Cooney ◽

Ali Shwaiki ◽

...

Keyword(s):

Lung Cancer ◽

Polymerase Chain Reaction ◽

Lymph Nodes ◽

Small Cell Lung Cancer ◽

Promoter Hypermethylation ◽

Small Cell ◽

Small Cell Lung ◽

Chain Reaction ◽

Specific Polymerase Chain Reaction ◽

Polymerase Chain

Abstract Context.— Even among cases of non–small cell lung cancer (NSCLC) in the most favorable stage (IA), the disease-specific mortality is 25% or greater. One plausible explanation implicates the simplistic standard pathologic procedures used to designate lymph node involvement. A more sensitive assessment of the nodal status may improve staging. Objective.—To determine the prognostic impact of detecting an abnormal molecular event (promoter hypermethylation in a set of relevant genes) in histologically uninvolved lymph nodes in resected NSCLC. Design.—In this retrospective analysis of archived material, we examined DNA extracted from lymph nodes of stage I NSCLC (n = 180). Patients underwent surgery between 1991 and 1995 in a single institution. Methylation-specific polymerase chain reaction was used to detect promoter hypermethylation in a panel of 8 genes. Survival data were extracted from the computerized database at the Tumor Registry. Results.—Evidence of promoter hypermethylation in at least 1 gene was detected in 67% of these N0 nodes. The most commonly hypermethylated gene was E-cadherin (53%). The hypermethylation frequency for the remaining genes were as follows: APC, 5%; p16, 9%; MGMT, 11%; hMLH1, 15%; RASSF1A, 4%; DAP kinase, 9%; and ATM, 19%. The presence of promoter hypermethylation in 2 or more genes did not influence the overall, median, or 5-year survival rates. Conclusions.—Identifying promoter hypermethylation (in our panel) in N0 lymph nodes in stage I NSCLC cannot be recommended for clinical decision making. Molecular abnormalities, including those found in cancer by qualitative methylation-specific polymerase chain reaction, are not synonymous with established, histologically detectable metastasis.

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