Experimental model of non–heart-beating donors: oxidative stress metabolism in kidney after cardiac arrest (30 minutes of warm ischemia) and reimplantation 24 hours later

PURPOSE: To describe technical aspects of a new experimental model that simulates a non heart beating organ donor. METHODS: Landrace pigs were operated on and cardiac arrest was obtained by means of myocardial infarction and interruption of ventilator support. RESULTS: Mean cardiac frequency, systolic and diastolic blood pressure levels, central venous pressure, oxygen saturation and concentration of expired CO2 dropout occurred at seven minutes after cardiac arrest. CONCLUSION: The procedure was easily reproduced and a homogeneous circulatory failure could de obtained by the end of seven minutes. The model is suitable for further studies regarding abdominal organ transplantation.

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Normoxic resuscitation after cardiac arrest protects against hippocampal oxidative stress, metabolic failure, and neuronal death

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0042 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S42-S42

Author(s):

Robert E Rosenthal ◽

Viktoria Vereczki ◽

Erica M Martin ◽

Gary Fiskum

Keyword(s):

Oxidative Stress ◽

Cardiac Arrest ◽

Neuronal Death

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McN-A-343, a muscarinic agonist, reduces inflammation and oxidative stress in an experimental model of ulcerative colitis

Life Sciences ◽

10.1016/j.lfs.2021.119194 ◽

2021 ◽

Vol 272 ◽

pp. 119194

Author(s):

Diva de Aguiar Magalhães ◽

Jalles Arruda Batista ◽

Stefany Guimarães Sousa ◽

Jayro dos Santos Ferreira ◽

Lauanda da Rocha Rodrigues ◽

...

Keyword(s):

Oxidative Stress ◽

Ulcerative Colitis ◽

Experimental Model ◽

Muscarinic Agonist ◽

And Oxidative Stress

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Usefulness of continuous hypothermic perfusion preservation for renal grafts from non-heart-beating donors with prolonged warm ischemia time

Transplantation Proceedings ◽

10.1016/s0041-1345(97)01034-8 ◽

1997 ◽

Vol 29 (8) ◽

pp. 3586-3587 ◽

Cited By ~ 8

Author(s):

K. Kozaki ◽

M. Uchiyama ◽

T. Nemoto ◽

H. Degawa ◽

N. Matsuno ◽

...

Keyword(s):

Warm Ischemia ◽

Warm Ischemia Time ◽

Ischemia Time ◽

Hypothermic Perfusion ◽

Heart Beating ◽

Renal Grafts

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2065 A COMPARISON OF THE OUTCOMES OF RENAL TRANSPLANTATION FROM HEART-BEATING CADAVERIC DONORS, AND NON-HEART-BEATING WHO PRESENT IRREVERSIBLE CARDIAC ARREST OCCURRING OUTSIDE THE HOSPITAL

The Journal of Urology ◽

10.1016/j.juro.2011.02.2338 ◽

2011 ◽

Vol 185 (4S) ◽

Author(s):

Felipe Villacampa ◽

José Medina-Polo ◽

Federico de la Rosa ◽

Manuel Pamplona ◽

Alfredo Rodríguez ◽

...

Keyword(s):

Cardiac Arrest ◽

Renal Transplantation ◽

Heart Beating ◽

Cadaveric Donors

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Liver Transplantation from Non-Heart-Beating Donors: Current Status and Future Prospects in an Experimental Model

Acta Chirurgica Belgica ◽

10.1080/00015458.2008.11680174 ◽

2008 ◽

Vol 108 (1) ◽

pp. 45-51 ◽

Cited By ~ 6

Author(s):

D. Monbaliu ◽

K. Vekemans ◽

Q. Liu ◽

V. Heedfeld ◽

T. Wylin ◽

...

Keyword(s):

Liver Transplantation ◽

Experimental Model ◽

Current Status ◽

Future Prospects ◽

Heart Beating

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Evaluation of the heme oxygenase-1 expression in esophagitis and esophageal cancer induced by different reflux experimental models and diethylnitrosamine

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000300015 ◽

2010 ◽

Vol 25 (3) ◽

pp. 304-310 ◽

Cited By ~ 6

Author(s):

Cleber Rosito Pinto Kruel ◽

Luis Felipe Ribeiro Pinto ◽

Tania Cristina Moita Blanco ◽

Theresa Christina Barja-Fidalgo ◽

Levi Lourenzo Melo ◽

...

Keyword(s):

Oxidative Stress ◽

Experimental Model ◽

Heme Oxygenase ◽

Inflammatory Cells ◽

Acid Reflux ◽

Experimental Models ◽

Control Group ◽

Heme Oxygenase 1 ◽

Duodenal Reflux ◽

Esophageal Carcinogenesis

PURPOSE: To study the expression of heme-oxygenase-1 (HO-1), an enzyme induced by oxidative stress, in specimens obtained from an experimental model in rats that evaluated the role of gastric and duodenal reflux in esophageal carcinogenesis. METHODS: Esophageal specimens embedded in paraffin obtained from different experimental groups of rats were used for immunohistochemistry analysis of HO-1 expression. The rats had been divided into the following groups and were killed after 22 weeks: (1) cardioplasty to induce acid reflux; (2) esophagoduodenal anastomosis to induce duodenal reflux; (3) no treatment; (4) cardioplasty + diethylnitrosamine (DEN); (5) esophagoduodenal anastomosis + DEN; and (6) DEN. The study sample comprised 3 specimens from each group with the most severe histopathological lesions found on each study branch. RESULTS: The expression of HO-1 was seen only in rat specimens submitted to esophagoduodenal anastomosis (Groups 2 and 5), and the analysis of mean fluorescence intensity revealed a significant increase of HO-1 expression (4.8 and 4.6 fold, respectively) when compared with the control group (Group 3) (p<0.05). The main target for HO-1 induction was the inflammatory cells inside the tumor or in subepithelial areas. Rats exposed to gastric reflux had no HO-1 expression. CONCLUSION: Reflux esophagitis induced by reflux of duodenal contents, which provoked considerable oxidative stress, may play an important role in esophageal carcinogenesis. Acid reflux did not induce oxidative stress in this experimental model.

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