Network Analysis for the Identification of Hub Genes and Related Molecules as Potential Biomarkers Associated With the Differentiation of Bone Marrow-derived Stem Cells Into Hepatocytes

The incidence of liver diseases has been increasing steadily. However, it has some shortcomings, such as high cost and organ donor scarcity. The application of stem cell research has brought new ideas for the treatment of liver diseases. Therefore, it is particularly important to clarify the molecular and regulatory mechanisms of differentiation of bone marrow-derived stem cells (BMSCs) into liver cells. Herein, we screened differentially expressed genes between hepatocytes and untreated BMSCs to identify the genes responsible for the differentiation of BMSCs into hepatocytes. GSE30419 gene microarray data of BMSCs and GSE72088 gene microarray data of primary hepatocytes were obtained from the Gene Expression Omnibus database. Transcriptome Analysis Console software showed that 1896 genes were upregulated and 2506 were downregulated in hepatocytes as compared with BMSCs. Hub genes were analyzed using the STRING, revealing that two hub genes, Cat and Cyp2e1, play a pivotal role in oxidation-reduction process. The results indicate that the lncRNA-miRNA-mRNA interaction chain may play an important role in the differentiation of BMSCs into hepatocytes, which provides a new therapeutic target for liver disease treatment.

Association between organ donation awareness and willingness among Saudi university students

Background: As the global incidence of end-stage organ failure increases, the gap between organ donors and recipients continues to widen. This study was conducted to determine the association between organ donation awareness and organ donation willingness among students of a Saudi university.Design and methods: This analytical cross-sectional study was conducted in King Faisal University, Al-Ahsa, Saudi Arabia. Multi-stage stratified random sampling was used to select the required 723 sample size. Estimation of proportion of students who were aware about organ donation and those who were willing to donate their organs was performed using EpiInfo© Version 7. Association between organ donation awareness and willingness was determined using multivariate logistic regression.Results: Nearly 98% of the participants were aware that organ donation saves lives (95% CI: 96.27%-98.53%); 84.09% were aware that Islam allows organ donation (95% CI: 81.25%-86.58%); 64.87% were aware that organ donation is successfully done in Saudi Arabia (95% CI: 61.32%-68.26%); 32.64% were aware that the government gives incentives to the family of an organ donor (95% CI: 29.32%-36.14%). Over half (56.71%) of the students (95% CI: 53.07 - 60.28%) expressed willingness to donate their organs. Positive association was found between awareness on the position of Islam on organ donation (OR=2.7023, p<0.0001) and awareness of that organ donation can be successfully performed in Saudi Arabia (OR=1.9447, p=0.0001).Conclusion: Awareness that Islam allows organ donation and awareness that organ donation is successfully done in Saudi Arabia increased the students’ willingness to donate.

An Inventory of Deceased Donor Family Care and Contact Between Donor Families and Recipients in 15 European Countries

Families of organ donors play an important role in the deceased organ donation process. The aim of this study was to gain insight into donor family care by creating an inventory of practice in various European countries. A questionnaire about donor family care and contact between donor families and recipients was developed. Representatives of the organ donor professionals of 15 European countries responded (94%). The donor coordinator plays a key role in care for the donor family. All countries provide information about the donation results to the families, although diminished due to privacy laws. Anonymous written contact between donor families and recipients is possible in almost all countries and direct contact in only a few. Remembrance ceremonies exist in most countries. Half of the respondents thought the aftercare could improve. This first inventory shows that differences exist between countries, depending on the organisation of the donation process, the law and the different role of the professionals. Direct contact between donor families and recipients is rarely supported by the donation organisation. To date there has been limited research about the experience of donor family aftercare and we would urge all donation organisations to consider this as a priority area.

Trauma Surgeons’ Perceptions of Resuscitating Lethally Injured Patients for Organ Preservation

Background Trauma surgeons face a challenge when deciding whether to resuscitate lethally injured patients whose organ donor status is unknown. Data suggests practice pattern variability in this setting, but little is known about why. Materials and Methods We conducted semi-structured interviews with trauma surgeons practicing in Level 1 or 2 trauma centers in Tennessee. Interviews focused on ethical dilemmas and resource constraints. Analysis was performed using inductive thematic analysis. Results Response rate was 73% (11/15). Four key themes emerged. All described resuscitating patients to buy time to collect more definitive clinical information and to identify family. Some acknowledged this served the secondary purpose of organ preservation. 11/11 participants felt a primacy of obligation to the patient in front of them even after it became apparent, they could not personally benefit. For 9/11 (82%), the moral obligation to consider organ preservation was secondary/balancing; 2/11 (18%) felt it was irrelevant/immoral. Resource allocation was commonly considered. All participants expressed some limitation to resources they would allocate. All participants conveyed clear moral agency in determining resuscitation extent when the goal was to save the patient’s life, however this was less clear when resuscitating for organ preservation. Across themes, perceptions of a “standard practice” existed but the described practices were not consistent across interviewees. Discussion Widely ranging perceptions regarding ethical and resource considerations underlie practices resuscitating toward organ preservation. Common themes suggest a lack of consensus. Despite expressed beliefs, there is no identifiable standard of practice amongst trauma surgeons resuscitating in this setting.

Application of Graphene in Tissue Engineering of the Nervous System

Graphene is the thinnest two-dimensional (2D), only one carbon atom thick, but one of the strongest biomaterials. Due to its unique structure, it has many unique properties used in tissue engineering of the nervous system, such as high strength, flexibility, adequate softness, electrical conductivity, antibacterial effect, and the ability to penetrate the blood–brain barrier (BBB). Graphene is also characterized by the possibility of modifications that allow for even wider application and adaptation to cell cultures of specific cells and tissues, both in vitro and in vivo. Moreover, by using the patient's own cells for cell culture, it will be possible to produce tissues and organs that can be re-transplanted without transplant rejection, the negative effects of taking immunosuppressive drugs, and waiting for an appropriate organ donor.

3D bioprinting in microgravity: An end to organ donor shortages?

Countless lives have been saved with the advent of modern organ transplantation. However, the current shortage of compatible organ donors is limiting the life-saving potential of transplantation. According to the United Network for Organ Sharing, approximately 20 patients die each day in the United States while waiting for a transplant [1]. The discrepancy between supply and demand of organ donors is accentuated by a fundamental ethical dilemma associated with deceased organ donation: one person must die so that another may live [2]. The current viewpoint considers the viability of 3D bioprinting in microgravity as a solution to organ donor shortages. Current alternatives to deceased organ donation, including xenotransplantation and other state-of-the-art bioprinting techniques, are reviewed and compared to bioprinting in microgravity. The limitations of bioprinting within Earth’s gravitational field are also discussed, revealing the need for further research.

The Effect of the Number and Identification of Recipients on Organ-Donation Decisions

We examined how presentations of organ donation cases in the media may affect people’s decisions about organ donation issues. Specifically, we focused on the combined effect of the information about the number of recipients saved by the organs of one deceased person (one vs. four) and the identifiability of the donor and the recipient(s) in organ donation descriptions, on people’s willingness to donate the organs of a deceased relative. Results suggest that reading about more people who were saved by the organs of a deceased donor does not increase willingness to donate. Replicating earlier research, we found that reading about a case of organ donation involving an identified deceased donor, deceased willingness to donate. However, this effect was attenuated when participants read about more recipients who were saved by the donation. Importantly, the presentation that prompted the greatest willingness to donate a deceased relative’s organs was the one that featured an unidentified donor and only one identified recipient. Finally, an explorative investigation into participants’ subconscious thoughts of death following the organ donation story revealed that identifying a deceased organ donor prompts more thoughts of death in the perceiver (regardless of the number of recipients).

SARS-CoV-2 Viral Load Assessment in Lung Transplantation

In the era of COVID-19 pandemic, organ transplantation programs were facing serious challenges. The lung transplantation donor pool was extremely limited and SARS-CoV-2 viral load assessment has become a crucial part of selecting an optimal organ donor. Since COVID-19 is a respiratory disease, the viral load is thought to be more important in lung transplantations as compared to other solid organ transplantations. We present two challenging cases of potential lung donors with a questionable COVID-19 status. Based on these cases, we suggest that the cycle threshold (Ct) value should always be requested from the laboratory and the decision whether to proceed with transplantation should be made upon complex evaluation of diverse criteria, including the nasopharyngeal swab and bronchoalveolar lavage PCR results, the Ct value, imaging findings and the medical history. However, as the presence of viral RNA does not ensure infectivity, it is still to be clarified which Ct values are associated with the viral viability. Anti-SARS-CoV-2 IgA antibodies may support the diagnosis and moreover, novel methods, such as quantifying SARS-CoV-2 nucleocapsid antigen in serum may provide important answers in organ transplantations and donor selections.