Chapter 7 Mass Isolation of Fly Tissues

1994 ◽  
pp. 129-142 ◽  
Author(s):  
Anthony P. Mahowald
Keyword(s):  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Steven D. Miller ◽  
Steven H. D. Haddock ◽  
William C. Straka ◽  
Curtis J. Seaman ◽  
Cynthia L. Combs ◽  
...  

AbstractMilky seas are a rare form of marine bioluminescence where the nocturnal ocean surface produces a widespread, uniform and steady whitish glow. Mariners have compared their appearance to a daylit snowfield that extends to all horizons. Encountered most often in remote waters of the northwest Indian Ocean and the Maritime Continent, milky seas have eluded rigorous scientific inquiry, and thus little is known about their composition, formation mechanism, and role within the marine ecosystem. The Day/Night Band (DNB), a new-generation spaceborne low-light imager, holds potential to detect milky seas, but the capability has yet to be demonstrated. Here, we show initial examples of DNB-detected milky seas based on a multi-year (2012–2021) search. The massive bodies of glowing ocean, sometimes exceeding 100,000 km2 in size, persist for days to weeks, drift within doldrums amidst the prevailing sea surface currents, and align with narrow ranges of sea surface temperature and biomass in a way that suggests water mass isolation. These findings show how spaceborne assets can now help guide research vessels toward active milky seas to learn more about them.


1989 ◽  
Vol 109 (6) ◽  
pp. 2869-2878 ◽  
Author(s):  
S Komesli ◽  
F Tournier ◽  
M Paintrand ◽  
R L Margolis ◽  
D Job ◽  
...  

Centrosomes from calf thymocytes were isolated using a simple preparative procedure that provides large yields of free organelles. A comparative study with centrosomes isolated from human cultured lymphoblasts has led to the discovery of important differences in the structure of the two isolates and in their capacity to nucleate microtubules from purified tubulin. The possibility that the centrosomal structure depends upon the growth state of cells is discussed.


1999 ◽  
Vol 68 (3) ◽  
pp. 355-361 ◽  
Author(s):  
Heide Brandhorst ◽  
Daniel Brandhorst ◽  
Bernhard J. Hering ◽  
Reinhard G. Bretzel

1975 ◽  
Vol 61 (4) ◽  
pp. 769 ◽  
Author(s):  
Anthony B. Bosworth ◽  
Imogene Schneider ◽  
Jerome E. Freier

Diabetes ◽  
1986 ◽  
Vol 35 (6) ◽  
pp. 649-653 ◽  
Author(s):  
C. Ricordi ◽  
E. H. Finke ◽  
P. E. Lacy
Keyword(s):  

1985 ◽  
Vol 77 (1) ◽  
pp. 155-165
Author(s):  
A. Tiedtke

A new procedure for mass isolation of pure pellicles containing intact basal bodies of Tetrahymena pyriformis is reported. The success of the procedure depends on the elimination of the sticky mucocyst contents before fractionation of the cells, which is induced by Alcian Blue 8GS. Under appropriate ionic conditions greater than 95% of the cells are able to form a capsule by simultaneous extrusion of all mature mucocysts. About 50% of these cells are able to escape from their capsules, which are now devoid of mature mucocysts. These cells are separated from the empty capsules and encapsulated cells by passage through layers of gauze of 10 microns pore size. The fractionation of mucocyst-free cells in homogenization buffer yields pure pellicles, which are retained when the homogenate is sieved through steel sieves of 5 microns pore size. Electron-microscopic controls show that the isolated pellicles are not contaminated with subcellular particles. Cells homogenized in the presence of low concentrations of Triton X-100 yield pellicles consisting of the known cell-surface-associated cytoskeletal elements, together with basal bodies. The cilia are detached just above the kinetosomal plate. The basal bodies of isolated pellicles are obviously undamaged, since all the known structures of native basal bodies are preserved. Even the granular matrix, a labile structure in the lumen of the basal body that probably contains RNA, is preserved.


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