Apoptosis within bovine follicular cells and its effect on oocyte development during in vitro maturation

Mechanisms of meiotic prophase I arrest maintenance (germinal vesicle [GV] stage) and meiotic resumption (germinal vesicle breakdown [GVBD] stage) in mammalian oocytes seem to be very complicated. These processes are regulated via multiple molecular cascades at transcriptional, translational, and posttranslational levels, and many of them are interrelated. There are many molecular cascades of meiosis maintaining and meiotic resumption in oocyte which are orchestrated by multiple molecules produced by pituitary gland and follicular cells. Furthermore, many of these molecular cascades are duplicated, thus ensuring the stability of the entire system. Understanding mechanisms of oocyte maturation is essential to assess the oocyte status, develop effective protocols of oocyte in vitro maturation, and design novel contraceptive drugs. Mechanisms of meiotic arrest maintenance at prophase I and meiotic resumption in mammalian oocytes are covered in the present article.

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The effect of sera, bovine serum albumin and follicular cells on in vitro maturation and fertilization of porcine oocytes

Theriogenology ◽

10.1016/0093-691x(92)90041-o ◽

1992 ◽

Vol 37 (4) ◽

pp. 779-790 ◽

Cited By ~ 37

Author(s):

Y.S. Zheng ◽

M.A. Sirard

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

In Vitro Maturation ◽

Follicular Cells

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Bovine herpesvirus 1 can impact the bovine oocyte development during in vitro maturation

Research in Veterinary Science ◽

10.1016/j.rvsc.2018.12.020 ◽

2019 ◽

Vol 123 ◽

pp. 135-140 ◽

Cited By ~ 5

Author(s):

Saullo V.P. Alves ◽

Eduardo P. da Costa ◽

Vanessa L.D. Queiroz-Castro ◽

Mariana Machado-Neves ◽

José D. Guimarães ◽

...

Keyword(s):

In Vitro Maturation ◽

Bovine Herpesvirus ◽

Oocyte Development ◽

Bovine Oocyte ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1

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Estrous cycle impacts microRNA content in extracellular vesicles that modulate bovine cumulus cell transcripts during in vitro maturation†

Biology of Reproduction ◽

10.1093/biolre/ioz177 ◽

2019 ◽

Vol 102 (2) ◽

pp. 362-375 ◽

Cited By ~ 8

Author(s):

Ana Clara Faquineli Cavalcante Mendes de Ávila ◽

Alessandra Bridi ◽

Gabriella Mamede Andrade ◽

Maite del Collado ◽

Juliano Rodrigues Sangalli ◽

...

Keyword(s):

Estrous Cycle ◽

Extracellular Vesicles ◽

In Vitro Maturation ◽

Mrna Translation ◽

Cumulus Cells ◽

Oocyte Development ◽

Follicle Cells ◽

Biological Processes ◽

Estrous Cycle Stage

Abstract Extracellular vesicles (EVs) are nanoparticles secreted by ovarian follicle cells. Extracellular vesicles are an important form of intercellular communication, since they carry bioactive contents, such as microRNAs (miRNAs), mRNAs, and proteins. MicroRNAs are small noncoding RNA capable of modulating mRNA translation. Thus, EVs can play a role in follicle and oocyte development. However, it is not clear if EV contents vary with the estrous cycle stage. The aim of this study was to investigate the bovine miRNA content in EVs obtained from follicles at different estrous cycle stages, which are associated with different progesterone (P4) levels in the follicular fluid (FF). We collected FF from 3 to 6 mm follicles and evaluated the miRNA profile of the EVs and their effects on cumulus-oocyte complexes during in vitro maturation. We observed that EVs from low P4 group have a higher abundance of miRNAs predicted to modulate pathways, such as MAPK, RNA transport, Hippo, Cell cycle, FoxO, oocyte meiosis, and TGF-beta. Additionally, EVs were taken up by cumulus cells and, thus, affected the RNA global profile 9 h after EV supplementation. Cumulus cells supplemented with EVs from low P4 presented upregulated genes that could modulate biological processes, such as oocyte development, immune responses, and Notch signaling compared with genes of cumulus cells in the EV free media or with EVs from high P4 follicles. In conclusion, our results demonstrate that EV miRNA contents are distinct in follicles exposed to different estrous cycle stage. Supplementation with EVs impacts gene expression and biological processes in cumulus cells.

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