Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa sorted by high speed flow cytometry

ABSTRACT Objective To evaluate the incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with sperm retrieval by epididymal aspiration (percutaneous epididymal sperm aspiration). Methods A case-control study comprising male children of couples in which the man had been previously vasectomized and chose vasectomy reversal (n=31) or in vitro fertilization with sperm retrieval by percutaneous epididymal sperm aspiration (n=30) to conceive new children, and a Control Group of male children of fertile men who had programmed vasectomies (n=60). Y-chromosome microdeletions research was performed by polymerase chain reaction on fathers and children, evaluating 20 regions of the chromosome. Results The results showed no Y-chromosome microdeletions in any of the studied subjects. The incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with spermatozoa recovered by percutaneous epididymal sperm aspiration did not differ between the groups, and there was no difference between control subjects born from natural pregnancies or population incidence in fertile men. Conclusion We found no association considering microdeletions in the azoospermia factor region of the Y chromosome and assisted reproduction. We also found no correlation between these Y-chromosome microdeletions and vasectomies, which suggests that the assisted reproduction techniques do not increase the incidence of Y-chromosome microdeletions.

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Sexual dimorphism in IVF bovine embryos produced by sperm sorted by high speed flow cytometry

Theriogenology ◽

10.1016/s0093-691x(98)90712-9 ◽

1998 ◽

Vol 49 (1) ◽

pp. 359

Author(s):

Z. Beyhan ◽

G.R. Welch ◽

N.L. First

Keyword(s):

Flow Cytometry ◽

Sexual Dimorphism ◽

High Speed ◽

Bovine Embryos ◽

High Speed Flow ◽

Speed Flow

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Comparison of flow cytometry-based apoptosis detection methods for measuring of human native and cryopreserved spermatozoids viability

Medical academic journal ◽

10.17816/maj19286 ◽

2020 ◽

Vol 20 (1) ◽

pp. 83-92

Author(s):

Anna A. Dotsenko ◽

Maria K. Serebriakova ◽

Igor V. Kudryavtsev ◽

Alexander N. Sukhachev ◽

Alexander V. Polevshchikov

Keyword(s):

Flow Cytometry ◽

In Vitro Fertilization ◽

High Efficiency ◽

Human Sperm ◽

Detection Methods ◽

Sperm Viability ◽

Vitro Fertilization ◽

Apoptosis Detection ◽

Narcissus Pseudonarcissus

The aim of the study was to compare the ability of various media for cryopreservation of sperm to ensure their viability after thawing and to assess the possibility of using Narcissus pseudonarcissus lectin to determine the viability of native and cryopreserved human sperm by flow cytometry. Materials and methods. Used ejaculate 54 men aged 26 to 47 years, undergoing treatment for infertility. The control was a native ejaculate, which was also used for the in vitro fertilization procedure. Four parallel samples were frozen using various commercial media. After storage and thawing, spermatozoa viability was assessed by flow cytometry using three dyes and Narcissus pseudonarcissus lectin. Results. All assays showed that cryopreservation led to a twofold decrease of sperm viability, dye to the changes in the composition and properties of cell membrane, decrease in mitochondrial membrane potential, as well as the damages of acrosomal complex and nucleus. The lowest decrease in sperm viability was shown for Quinns advantage sperm freezing medium for cryopreservation. Conclusion. Flow cytometry makes it possible to evaluate with high efficiency sperm viability as the part of in vitro fertilization. The results of viability assessment using daffodil lectin make the prediction of in vitro fertilization outcome more accurate.

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