Atomic force microscopy of plant cell walls, plant cell wall polysaccharides and gels

1997 ◽  
Vol 21 (1-2) ◽  
pp. 61-66 ◽  
Author(s):  
V.J Morris ◽  
A.P Gunning ◽  
A.R Kirby ◽  
A Round ◽  
K Waldron ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Plant Cell Walls ◽  
Cell Wall Polysaccharides ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1263
Author(s):  
David Stuart Thompson ◽  
Azharul Islam
Keyword(s):  
Cell Wall ◽  
Water Content ◽  
Bacterial Cellulose ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Plant Cell Walls ◽  
Cell Wall Polysaccharides ◽  
Degree Of Hydration ◽  
Cellulose Composites

The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls. Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions. Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition. Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition. Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience.

Subsurface Image Analysis of Plant Cell Wall with Atomic Force Microscopy

2013 ◽  
Vol 8 (2) ◽  
pp. 100-104
Author(s):  
Sahar Maghsoudy-Louyeh ◽  
Jeong Kim ◽  
Matthew Kropf ◽  
Bernhard Tittmann
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Image Analysis ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Force Microscopy ◽  
Atomic Force

Levels of plant cell wall structural organization revealed by atomic force microscopy

2008 ◽  
Vol 232 (3) ◽  
pp. 508-510 ◽  
Author(s):  
K. RADOTIĆ ◽  
D. DJIKANOVIĆ ◽  
J. BOGDANOVIĆ ◽  
D. VASILJEVIĆ-RADOVIĆ
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Plant Cell ◽  
Structural Organization ◽  
Plant Cell Wall ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Visualization of plant cell walls by atomic force microscopy

1996 ◽  
Vol 70 (3) ◽  
pp. 1138-1143 ◽  
Author(s):  
A.R. Kirby ◽  
A.P. Gunning ◽  
K.W. Waldron ◽  
V.J. Morris ◽  
A. Ng
Keyword(s):  
Atomic Force Microscopy ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals A Label-free, Fast and High-specificity Technique for Plant Cell Wall Imaging and Composition Analysis

2020 ◽  
Author(s):  
Huimin Xu ◽  
Yuanyuan Zhao ◽  
Yuanzhen Suo ◽  
Yayu Guo ◽  
Yi Man ◽  
...  
Keyword(s):  
Cell Wall ◽  
Chemical Composition ◽  
Raman Scattering ◽  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Wall ◽  
Composition Analysis ◽  
Plant Cell Walls ◽  
Label Free ◽  
Wall Imaging

Abstract Background: Cell wall imaging can considerably permit direct visualization of the molecular architecture of cell walls and provide the detailed chemical information on wall polymers, which is imperative to better exploit and use the biomass polymers; however, detailed imaging and quantifying of the native composition and architecture in the cell wall remains challenging.Results: Here, we describe a label-free imaging technology, coherent Raman scattering microscopy (CRS), including coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy, which images the major structures and chemical composition of plant cell walls. The major steps of the procedure are demonstrated, including sample preparation, setting the mapping parameters, analysis of spectral data, and image generation. Applying this rapid approach, which will help researchers understand the highly heterogeneous structures and organization of plant cell walls.Conclusions: This method can potentially be incorporated into label-free microanalyses of plant cell wall chemical composition based on the in situ vibrations of molecules.

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