NMR studies of metal ion binding to the Zn-finger-like HNH motif of colicin E9

2000 ◽  
Vol 79 (1-4) ◽  
pp. 365-370 ◽  
Author(s):  
Jonathan P Hannan ◽  
Sara B.-M Whittaker ◽  
Andrew M Hemmings ◽  
Richard James ◽  
Colin Kleanthous ◽  
...  
Keyword(s):  
Metal Ion ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Nmr Studies ◽  
Colicin E9

Quadrupolar metal ion NMR studies of metalloproteins

1998 ◽  
Vol 76 (2-3) ◽  
pp. 210-222 ◽  
Author(s):  
James M Aramini ◽  
Hans J Vogel
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Metal Ion ◽  
Chemical Information ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Nmr Studies ◽  
Small Proteins ◽  
Ion Binding Sites ◽  
Metal Ion Binding Sites

We present a summary of the quadrupolar metal ion NMR studies of metalloproteins conducted in our laboratory in recent years. The approaches we employ can be subdivided into two categories: (i) the use of low-frequency metal nuclei to probe metal ion binding sites in small proteins, exemplified by 43Ca NMR studies of alpha-lactalbumins and calcium-binding lysozymes, and (ii) the novel detection of the central transition of half-integer quadrupolar nuclei of moderate frequency bound to large metalloproteins, typified by 27Al, 45Sc, 69,71Ga, and 51V NMR studies of the transferrins. We highlight the chemical information regarding the nature of metal ion binding sites that can be obtained from this technique and emphasize the salient parameters that an investigator must consider to successfully apply quadrupolar NMR to the study of biological macromolecules.Key words: quadrupolar NMR, metalloproteins, transferrins.

scholarly journals Probing metal ion binding and conformational properties of the colicin E9 endonuclease by electrospray ionization time-of-flight mass spectrometry

2009 ◽  
Vol 11 (7) ◽  
pp. 1738-1752 ◽  
Author(s):  
Ewald T.J. van den Bremer ◽  
Wim Jiskoot ◽  
Richard James ◽  
Geoffrey R. Moore ◽  
Colin Kleanthous ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Metal Ion ◽  
Time Of Flight ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Conformational Properties ◽  
Colicin E9

Photophysical Characterisation, Metal Ion Binding and Enantiomeric Recognition of Chiral Ligands Containing Phenazine Fluorophore

2004 ◽  
Vol 69 (4) ◽  
pp. 885-896 ◽  
Author(s):  
Luisa Stella Dolci ◽  
Péter Huszthy ◽  
Erika Samu ◽  
Marco Montalti ◽  
Luca Prodi ◽  
...  
Keyword(s):  
Metal Ion ◽  
Photophysical Properties ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Ammonium Ions ◽  
Enantiomerically Pure ◽  
Binding Process ◽  
High Affinity ◽  
Enantiomeric Recognition ◽  
Chiral Species

Enantiomerically pure dimethyl- and diisobutyl-substituted phenazino-18-crown-6 ligands bind metal and ammonium ions and also primary aralkylammonium perchlorates in acetonitrile with high affinity, causing pronounced changes in their luminescence properties. In addition, they show enantioselectivity towards chiral primary aralkylammonium perchlorates. The possibility to monitor the binding process by photoluminescence spectroscopy can gain ground for the design of very efficient enantioselective chemosensors for chiral species. The observed changes in the photophysical properties are also an important tool for understanding the interactions present in the adduct.

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