Regulation by an extract of embryonic chick brain of the densities of voltage-dependent Na+ and Ca2+ channels in embryonic chick skeletal muscle cells during their development in culture

1998 ◽  
Vol 110 (2) ◽  
pp. 261-267
Author(s):  
Ryohei Satoh ◽  
Yumiko Nakabayashi ◽  
Takashi Katakura ◽  
Suteo Yamazaki
1967 ◽  
Vol 35 (2) ◽  
pp. 445-453 ◽  
Author(s):  
Y. Shimada ◽  
D. A. Fischman ◽  
A. A. Moscona

Dissociated myoblasts from 12-day chick embryos were cultured in monolayer, and the differentiation of skeletal muscle cells was studied by electron microscopy. The results have revealed a striking ultrastructural similarity between the in vivo and the in vitro developing muscle, particularly with respect to the myofibrils and sarcoplasmic reticulum. This study demonstrates that all the characteristic organelles of mature skeletal muscle can develop in vitro in the absence of nerves.


1992 ◽  
Vol 201 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Shanta V. Kharadia ◽  
Ted W. Huiatt ◽  
Hui-Yu Huang ◽  
Jon E. Peterson ◽  
Donald J. Graves

1979 ◽  
Vol 81 (1) ◽  
pp. 59-66 ◽  
Author(s):  
T Obinata ◽  
Y Shimada ◽  
R Matsuda

The fine structurel distribution of troponin on thin filaments in developing myofibrils was investigated by the use of immunoelectron microscopy. Embryonic chick skeletal muscle cells grown in vitro were treated with antibodies against each of the troponin components (troponin T, I, and C) from adult chicken muscles. Each antibody was distributed along the thin filaments with a period of 38 nm. It is concluded that these newly synthesized regulatory proteins are assembled at their characteristic position from the initial phases of myofibrillogenesis.


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