Isolation and characterization of the promoter region of the human GM3 synthase gene

Author(s):  
Sang-Wan Kim ◽  
Sang-Hyeon Lee ◽  
Kyoung-Sook Kim ◽  
Cheorl-Ho Kim ◽  
Young-Kug Choo ◽  
...  



Author(s):  
Lynn Taylor ◽  
Xiangdong Liu ◽  
William Newsome ◽  
Richard A. Shapiro ◽  
Maithreyan Srinivasan ◽  
...  




2001 ◽  
Vol 281 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Masanobu Yamada ◽  
Atsushi Ozawa ◽  
Sumiyasu Ishii ◽  
Nobuyuki Shibusawa ◽  
Tetsu Hashida ◽  
...  


1999 ◽  
Vol 181 (2) ◽  
pp. 670-674 ◽  
Author(s):  
Karinne De Pina ◽  
Valerie Desjardin ◽  
Marie-Andree Mandrand-Berthelot ◽  
Gerard Giordano ◽  
Long-Fei Wu

ABSTRACT Expression of the nickel-specific transport system encoded by theEscherichia coli nikABCDE operon is repressed by a high concentration of nickel. By using random transposon Tn10 insertion, we isolated mutants in which expression of the nik operon became constitutive with respect to nickel. We have identified the corresponding nikR gene which encodes a nickel-responsive regulator. Expression of nikRwas partially controlled by Fnr through transcription from thenikA promoter region. In addition, a specific transcription start site for the constitutive expression of nikR was found 51 bp upstream of the nikR gene.



1998 ◽  
Vol 17 (4) ◽  
pp. 359-376 ◽  
Author(s):  
JYH-YIH CHEN ◽  
HSIU-LAN TSAI ◽  
CHI-YAO CHANG ◽  
JYH-I WANG ◽  
SHIH-CHIEH SHEN ◽  
...  


1995 ◽  
Vol 118 (4) ◽  
pp. 725-733 ◽  
Author(s):  
Se-Ho Park ◽  
Jeong Ho Yoon ◽  
Hyun-Ah Cho ◽  
Yeong Do Kwon ◽  
Rho Hyun Seong ◽  
...  


Genome ◽  
2016 ◽  
Vol 59 (1) ◽  
pp. 23-36 ◽  
Author(s):  
Julia Lambret-Frotté ◽  
Sinara Artico ◽  
Sarah Muniz Nardeli ◽  
Fernando Fonseca ◽  
Osmundo Brilhante Oliveira-Neto ◽  
...  

Cotton is one of the most economically important cultivated crops. It is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. Therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. The isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified crops. One of the major drawbacks in cotton production is pest damage; therefore, the most promising, cost-effective, and sustainable method for pest control is the development of genetically resistant cotton lines. Considering this scenario, our group isolated and characterized the promoter region of a MCO (multicopper oxidase) from Gossypium hirsutum, named GhAO-like1 (ascorbate oxidase-like1). The quantitative expression, together with the in vivo characterization of the promoter region reveals that GhAO-like1 has a flower- and fruit-specific expression pattern. The GUS activity is mainly observed in stamens, as expected considering that the GhAO-like1 regulatory sequence is enriched in cis elements, which have been characterized as a target of reproductive tissue specific transcription factors. Both histological and quantitative analyses in Arabidopsis thaliana have confirmed flower (mainly in stamens) and fruit expression of GhAO-like1. In the present paper, we isolated and characterized both in silico and in vivo the promoter region of the GhAO-like1 gene. The regulatory region of GhAO-like1 might be useful to confer tissue-specific expression in genetically modified plants.





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