Molecular cloning and characterisation of a mouse gene encoding an isoform of the neuronal cyclin-dependent kinase 5 (CDK5) activator

Breast cancer affects women at relatively high frequency (1). We mined published microarray datasets (2, 3) to determine in an unbiased fashion and at the systems level genes most differentially expressed in the primary tumors of patients with breast cancer. We report here significant differential expression of the gene encoding cyclin dependent kinase 5, CDK5, when comparing primary tumors of the breast to the tissue of origin, the normal breast. CDK5 mRNA was present at significantly higher quantities in tumors of the breast as compared to normal breast tissue. Analysis of human survival data revealed that expression of CDK5 in primary tumors of the breast was correlated with distant metastasis-free survival in patients with luminal A subtype cancer. CDK5 may be of relevance to initiation, maintenance or progression of cancers of the female breast.

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Molecular Cloning and Chromosomal Mapping of the Mouse Cyclin-Dependent Kinase 5 Gene

Genomics ◽

10.1006/geno.1995.1194 ◽

1995 ◽

Vol 28 (3) ◽

pp. 585-588 ◽

Cited By ~ 19

Author(s):

Toshio Ohshima ◽

James W. Nagle ◽

Harish C. Pant ◽

Jay B. Joshi ◽

Christine A. Kozak ◽

...

Keyword(s):

Molecular Cloning ◽

Chromosomal Mapping ◽

Cyclin Dependent Kinase ◽

Cyclin Dependent Kinase 5

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Hypoxic preconditioning reduces expression of the cyclin dependent kinase 5 in the post-ischemic neurons

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/sj.jcbfm.9591524.0309 ◽

2005 ◽

Vol 25 (1_suppl) ◽

pp. S309-S309

Author(s):

Svetlana Pundik ◽

W David Lust ◽

Jose Valerio ◽

Michael Buczek ◽

Randall D York ◽

...

Keyword(s):

Hypoxic Preconditioning ◽

Cyclin Dependent Kinase ◽

Cyclin Dependent Kinase 5

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Homology Modeling of Mus Musculus CDK5 and Molecular Docking Studies with Flavonoids

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i6.8779 ◽

2017 ◽

Vol 9 (6) ◽

Author(s):

Sowmya Suri ◽

Rumana Waseem ◽

Seshagiri Bandi ◽

Sania Shaik

Keyword(s):

Molecular Docking ◽

3D Model ◽

Structural Features ◽

Docking Studies ◽

Amino Acid Residues ◽

Cyclin Dependent Kinase ◽

Ligand Complex ◽

Ligand Interaction ◽

Molecular Docking Studies ◽

Cyclin Dependent Kinase 5

A 3D model of Cyclin-dependent kinase 5 (CDK5) (Accession Number: Q543f6) is generated based on crystal structure of P. falciparum PFPK5-indirubin-5-sulphonate ligand complex (PDB ID: 1V0O) at 2.30 Å resolution was used as template. Protein-ligand interaction studies were performed with flavonoids to explore structural features and binding mechanism of flavonoids as CDK5 (Cyclin-dependent kinase 5) inhibitors. The modelled structure was selected on the basis of least modeler objective function. The model was validated by PROCHECK. The predicted 3D model is reliable with 93.0% of amino acid residues in core region of the Ramachandran plot. Molecular docking studies with flavonoids viz., Diosmetin, Eriodictyol, Fortuneletin, Apigenin, Ayanin, Baicalein, Chrysoeriol and Chrysosplenol-D with modelled protein indicate that Diosmetin is the best inhibitor containing docking score of -8.23 kcal/mol. Cys83, Lys89, Asp84. The compound Diosmetin shows interactions with Cys83, Lys89, and Asp84.

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