On the useful role of OH free radicals in differentiation of cultured human fibroblasts

2000 ◽  
Vol 31 (3) ◽  
pp. 233-242 ◽  
Author(s):  
Enikö Bazsó-Dombi ◽  
Katalin Oravecz ◽  
Florence Jeney ◽  
Katalin Nagy ◽  
Imre Zs.-Nagy
Keyword(s):  
Free Radicals ◽  
Human Fibroblasts ◽  
Cultured Human Fibroblasts

scholarly journals Vitamin E activates CRABP-II gene expression in cultured human fibroblasts, role of protein kinase C

FEBS Letters ◽  
2004 ◽  
Vol 569 (1-3) ◽  
pp. 240-244 ◽  
Author(s):  
Amparo Gimeno ◽  
Rosa Zaragozá ◽  
Juan R Viña ◽  
Vicente J Miralles
Keyword(s):  
Gene Expression ◽  
Protein Kinase C ◽  
Vitamin E ◽  
Protein Kinase ◽  
Human Fibroblasts ◽  
Kinase C ◽  
Cultured Human Fibroblasts ◽  
Crabp Ii

scholarly journals A key role of PGC-1α transcriptional coactivator in production of VEGF by a novel angiogenic agent COA-Cl in cultured human fibroblasts

2016 ◽  
Vol 4 (6) ◽  
pp. e12742 ◽  
Author(s):  
Junsuke Igarashi ◽  
Ryuji Okamoto ◽  
Tetsuo Yamashita ◽  
Takeshi Hashimoto ◽  
Sakiko Karita ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
Transcriptional Coactivator ◽  
Cultured Human Fibroblasts

Acrylic bone cement induces the production of free radicals by cultured human fibroblasts

Biomaterials ◽  
1997 ◽  
Vol 18 (16) ◽  
pp. 1133-1135 ◽  
Author(s):  
Fernando Martins Vale ◽  
Matilde Castro ◽  
Jacinto Monteiro ◽  
Frederico S. Couto ◽  
Rui Pinto ◽  
...  
Keyword(s):  
Free Radicals ◽  
Bone Cement ◽  
Human Fibroblasts ◽  
Acrylic Bone Cement ◽  
Cultured Human Fibroblasts

scholarly journals Characterization of the Coagulant Activity of Cultured Human Fibroblasts

Blood ◽  
1971 ◽  
Vol 37 (1) ◽  
pp. 47-51 ◽  
Author(s):  
DAVID GREEN ◽  
CATHY RYAN ◽  
NANCY MALANDRUCCOLO ◽  
HENRY L. NADLER
Keyword(s):  
Factor Viii ◽  
Tissue Factor ◽  
Human Fibroblasts ◽  
Wide Distribution ◽  
Skin Fibroblasts ◽  
Factor Vii ◽  
Normal Individuals ◽  
Coagulant Activity ◽  
Cultured Human Fibroblasts

Abstract Skin fibroblasts were cultured from normal individuals, from patients with hemophilia, and from known and suspected carriers of hemophilia. All cell lysates studied had similar coagulant activity unaffected by incubation with a specific antibody to factor VIII and, therefore, presumably not factor VIII. Further investigation indicated that the coagulant had many properties indistinguishable from those associated with "tissue factor," including heat lability, sedimentation characteristics, and dependency on factor VII for expression of coagulant activity. The presence of tissue factor in skin fibroblasts is consistent with the wide distribution of this coagulant, and the ability to maintain this material in tissue culture provides an opportunity for further investigation of the role of tissue factor in normal and pathologic states.

scholarly journals Modulation of fibroblast proliferation by oxygen free radicals

1990 ◽  
Vol 265 (3) ◽  
pp. 659-665 ◽  
Author(s):  
G A C Murrell ◽  
M J O Francis ◽  
L Bromley
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Oxygen Free Radicals ◽  
Human Fibroblasts ◽  
Free Radical Scavengers ◽  
Fibroblast Proliferation ◽  
Inhibition Of Proliferation ◽  
Cultured Human Fibroblasts ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition

The major unexplained phenomenon in fibrotic conditions is an increase in replicating fibroblasts. In this report we present evidence that oxygen free radicals can both stimulate and inhibit proliferation of cultured human fibroblasts, and that fibroblasts themselves release superoxide (O2.-) free radicals. Fibroblasts released O2.- in concentrations which stimulated proliferation, a finding confirmed by a dose-dependent inhibition of proliferation by free radical scavengers. Oxygen free radicals released by a host of agents may thus provide a very fast, specific and sensitive trigger for fibroblast proliferation. Prolonged stimulation may result in fibrosis, and agents which inhibit free radical release may have a role in the prevention of fibrosis.

Regulation of purine de novo synthesis in cultured human fibroblasts: The role of

1979 ◽  
Vol 562 (1) ◽  
pp. 162-176 ◽  
Author(s):  
Ross B. Gordon ◽  
Lambert Thompson ◽  
Lambro A. Johnson ◽  
Bryan T. Emmerson
Keyword(s):  
De Novo ◽  
Human Fibroblasts ◽  
De Novo Synthesis ◽  
Cultured Human Fibroblasts

scholarly journals Sumoylation of the major immediate-early IE2 protein of human cytomegalovirus Towne strain is not required for virus growth in cultured human fibroblasts

2004 ◽  
Vol 85 (8) ◽  
pp. 2149-2154 ◽  
Author(s):  
Hye-Ra Lee ◽  
Jin-Hyun Ahn
Keyword(s):  
Human Cytomegalovirus ◽  
Human Fibroblasts ◽  
Artificial Chromosome ◽  
Immediate Early ◽  
Nuclear Antigen ◽  
Virus Growth ◽  
Cultured Human Fibroblasts ◽  
Proliferating Cell ◽  
Revertant Virus

Sumoylation of the major immediate-early IE2 protein of human cytomegalovirus has been shown to increase transactivation activity in target reporter gene assays. This study examined the role of IE2 sumoylation in viral infection. A Towne strain-based bacterial artificial chromosome clone was generated encoding a mutated form of the IE2 protein with Lys→Arg substitutions at positions 175 and 180, the two major sumoylation sites. When human fibroblast (HF) cells were infected with the reconstituted mutant virus, (i) viral growth kinetics, (ii) the accumulation of IE1 (UL123), IE2 (UL122), p52 (UL44) and pp65 (UL83) proteins and (iii) the relocalization of the cellular small ubiquitin-like modifier (SUMO)-1, p53 and proliferating cell nuclear antigen proteins into viral DNA replication compartments were comparable with those of the wild-type and the revertant virus. The data demonstrate that sumoylation of IE2 is not essential for virus growth in cultured HF cells.

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