The use of multi-parameter flow cytometry to compare the physiological response of Escherichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations

1999 ◽  
Vol 75 (2-3) ◽  
pp. 251-264 ◽  
Author(s):  
Christopher J Hewitt ◽  
Gerhard Nebe-Von Caron ◽  
Alvin W Nienow ◽  
Caroline M McFarlane
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Continuous Culture ◽  
Physiological Response ◽  
Fed Batch ◽  
Glucose Limitation

scholarly journals Glucose transport as rate-limiting step in the growth of Escherichia coli on glucose

1976 ◽  
Vol 156 (2) ◽  
pp. 477-480 ◽  
Author(s):  
D Herbert ◽  
H L Kornberg
Keyword(s):  
Escherichia Coli ◽  
Continuous Culture ◽  
Glucose Transport ◽  
Growth Rates ◽  
Glucose Limitation ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Wide Range ◽  
Rate Limiting

Over a wide range of growth rates, two strains of Escherichia coli growing aerobically in continuous culture under glucose limitation utilized glucose at rates identical with those at which cells harvested from the chemostats transported [14C]glucose.

scholarly journals Glucose transport of Escherichia coli growing in glucose-limited continuous culture

1979 ◽  
Vol 178 (1) ◽  
pp. 97-101 ◽  
Author(s):  
I S Hunter ◽  
H L Kornberg
Keyword(s):  
Escherichia Coli ◽  
Continuous Culture ◽  
Glucose Concentration ◽  
Wild Type ◽  
Phosphotransferase System ◽  
Glucose Limitation ◽  
Low Glucose ◽  
Rate Limiting ◽  
Excess Glucose ◽  
Stimulation Of

Dilute cultures of wild-type Escherichia coli K12 and of derivatives impaired in one or other Enzyme-II component of the glucose phosphotransferase system were grown in continuous culture under glucose limitation. Cells harvested from the chemostat took up [U-14C]glucose from 0.1 mM solutions at rates directly related to the rates at which those cells had grown; the activity of the phosphotransferase system in those cells, rendered permeable with optimal accounts of toluene, parallels the ability of the cells to take up glucose. The capacity of these systems was rate-limiting for growth under the negligibly low glucose concentration in the chemostat, but was adequate to account for the stimulation of respiration observed when the cells were presented suddenly with excess glucose.

DNA replication in Escherichia coli gyrB(Ts) mutants analysed by flow cytometry

1991 ◽  
Vol 142 (2-3) ◽  
pp. 223-227 ◽  
Author(s):  
U. von Freiesleben ◽  
K.V. Rasmussen
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Dna Replication

scholarly journals Occurrence of Escherichia coli O157:H7 in river water determined by flow cytometry.

1998 ◽  
Vol 13 (2) ◽  
pp. 77-83 ◽  
Author(s):  
YASUNOBU TANAKA ◽  
MASATO YOSHIMITSU ◽  
NOBUYASU YAMAGUCHI ◽  
KATSUJI TANI ◽  
MASAO NASU
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
River Water ◽  
Escherichia Coli O157
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