Determination of the intracellular element concentrations in cultured H9c2 cells by proton microprobe techniques

Author(s):  
J.A Quaedackers ◽  
S van Engeland ◽  
P.H.A Mutsaers ◽  
J.J.M de Goeij ◽  
L.H.E.H Snoeckx ◽  
...  
Author(s):  
H.J. Dudek

The chemical inhomogenities in modern materials such as fibers, phases and inclusions, often have diameters in the region of one micrometer. Using electron microbeam analysis for the determination of the element concentrations one has to know the smallest possible diameter of such regions for a given accuracy of the quantitative analysis.In th is paper the correction procedure for the quantitative electron microbeam analysis is extended to a spacial problem to determine the smallest possible measurements of a cylindrical particle P of high D (depth resolution) and diameter L (lateral resolution) embeded in a matrix M and which has to be analysed quantitative with the accuracy q. The mathematical accounts lead to the following form of the characteristic x-ray intens ity of the element i of a particle P embeded in the matrix M in relation to the intensity of a standard S


2016 ◽  
Vol 127 ◽  
pp. 237-246 ◽  
Author(s):  
Wenjun Li ◽  
Changle Wang ◽  
Bingyu Gao ◽  
Yating Wang ◽  
Xindi Jin ◽  
...  

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