2,7-Diaminofluorene is a sensitive substrate for detection and characterization of plant root peroxidase activities

Plant Science ◽  
2001 ◽  
Vol 161 (6) ◽  
pp. 1063-1066 ◽  
Author(s):  
Stéven Criquet ◽  
Erik J Joner ◽  
Corinne Leyval
Keyword(s):  
2021 ◽  
Vol 9 (2) ◽  
pp. 229
Author(s):  
Martti Vasar ◽  
John Davison ◽  
Siim-Kaarel Sepp ◽  
Maarja Öpik ◽  
Mari Moora ◽  
...  

Deserts cover a significant proportion of the Earth’s surface and continue to expand as a consequence of climate change. Mutualistic arbuscular mycorrhizal (AM) fungi are functionally important plant root symbionts, and may be particularly important in drought stressed systems such as deserts. Here we provide a first molecular characterization of the AM fungi occurring in several desert ecosystems worldwide. We sequenced AM fungal DNA from soil samples collected from deserts in six different regions of the globe using the primer pair WANDA-AML2 with Illumina MiSeq. We recorded altogether 50 AM fungal phylotypes. Glomeraceae was the most common family, while Claroideoglomeraceae, Diversisporaceae and Acaulosporaceae were represented with lower frequency and abundance. The most diverse site, with 35 virtual taxa (VT), was in the Israeli Negev desert. Sites representing harsh conditions yielded relatively few reads and low richness estimates, for example, a Saudi Arabian desert site where only three Diversispora VT were recorded. The AM fungal taxa recorded in the desert soils are mostly geographically and ecologically widespread. However, in four sites out of six, communities comprised more desert-affiliated taxa (according to the MaarjAM database) than expected at random. AM fungal VT present in samples were phylogenetically clustered compared with the global taxon pool, suggesting that nonrandom assembly processes, notably habitat filtering, may have shaped desert fungal assemblages.


Author(s):  
Ajay Singhvi ◽  
Bo Ma ◽  
Johannes Daniel Scharwies ◽  
Jose R. Dinneny ◽  
Butrus T. Khuri-Yakub ◽  
...  
Keyword(s):  

Plant Disease ◽  
2021 ◽  
Author(s):  
Nimllash Thangam Sivachandra Kumar ◽  
Kiela B Caudillo-Ruiz ◽  
Syama Chatterton ◽  
Sabine Banniza

Aphanomyces root rot, caused by the soil-borne oomycete Aphanomyces euteiches Drechs., has developed into a serious disease in the pea and lentil-producing areas of the Great Plains of North America. Based on six pea differentials previously used to differentiate 11 pathotypes in France, pathotypes were identified among field isolates from Saskatchewan (14) and Alberta (18). Four isolates from the USA and standard isolates for pathotypes I and III designated in the French study were also included. Each isolate was tested twice in replicated experiments by inoculating French pea differentials Baccara, Capella, MN 313, 902131, 552 and PI 80693, along with the Canadian susceptible pea cultivar CDC Meadow and partially resistant USDA line PI 660736 under controlled conditions. Pea plants grown in vermiculite were inoculated 10 days after seeding by pipetting 5 mL of a suspension containing 1 x 103 zoospores mL-1 to the base of each plant. Root discoloration was scored 10 days post-inoculation using a 0-5 scale. Testing revealed that 38 of the isolates, including standard pathotype I isolate RB84 belonged to pathotype I, 4 isolates including standard pathotype III isolate Ae109 were pathotype III, and USA isolate Ae16-01 was a pathotype II isolate. An alfalfa isolate from Quebec was avirulent on all pea genotypes. These findings indicate that pathotype type I is predominant on the Canadian prairies.


2018 ◽  
Vol 47 (2) ◽  
pp. I-VIII
Author(s):  
Radu E. SESTRAS

Notulae Botanicae Horti Agrobotanici Cluj-Napoca (NBHA), Issue 2, Volume 47, 2019: The papers published in this issue Vol 47 No 2 (2019) represent new exciting researches in different topics of life science, respectively in plant science, horticulture, agronomy and crop science. Among the interesting articles we invite you to find news about temporary immersion system for micropropagation of tree species and plant root hair growth in response to hormones (reviews); sexual and vegetative propagation of Hypericum empetrifolium Willd. subsp. empetrifolium; cloning and functional characterization of CsUGD2 in cucumber (Cucumis sativus L.); potential transference of CP4 EPSPS to weed species from genetically modified Gossypium hirsutum in Northern Mexico; isolation and functional characterization of an AGAMOUS-LIKE 18 (AGL18) MADS-box gene from cucumber (Cucumis sativus L.); avocado fruit pulp transcriptomes in the after-ripening process; transcriptome sequencing of two Kentucky bluegrass (Poa pratensis L.) genotypes in response to heat stress etc. Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal has moved to online-only publication at the start of 2017; beginning in 2019, the journal appears quarterly. At the same time, we maintain our standard publication, as printed form, with ‘classic’ style - volume, issue, pagination.


Microbiology ◽  
1994 ◽  
Vol 140 (6) ◽  
pp. 1377-1387 ◽  
Author(s):  
R. De Mot ◽  
G. Schoofs ◽  
A. Roelandt ◽  
P. Declerck ◽  
P. Proost ◽  
...  

Author(s):  
Oluwaseun Adeyinka Fasusi ◽  
Adenike Eunice Amoo ◽  
Olubukola Oluranti Babalola

AbstractThe region around the plant root referred to as the rhizosphere, is the zone where various microbial activity occurs. It performs crucial functions such as increasing the uptake of nutrients for plant development and preventing plant against plant pathogens. Keeping in mind the beneficial role performed by rhizospheric microorganisms, rhizobacterial species were isolated from the maize and soybean plant's rhizosphere. The isolated microorganisms were evaluated for their biochemical characteristics, plant growth-promoting potentials, tolerance to different environmental conditions, and their antifungal activity against Fusarium graminearum, a fungal pathogen that infects maize. The rhizobacterial isolates with multiple plant growth-promoting potentials were identified as Bacillus spp (80.77%), Rhodocyclaceae bacterium (3.85%), Enterococcus spp (3.85%). Massilia spp (3.85%. and Pseudomonas (7.69%) species based on their 16S rRNA molecular characterization. The bacterial isolates possessed antifungal activities against Fusarium graminearum, promote maize and soybeans seed under laboratory conditions, and exhibited different levels of tolerance to pH, temperature, salt, and heavy metal. Based on this, the whole genome sequencing of Bacillus sp. OA1, Pseudomonas rhizosphaerea OA2, and Pseudomonas sp. OA3 was performed using Miseq Illumina system to determine the functional genes and secondary metabolites responsible for their plant growth-promoting potential Thus, the result of this research revealed that the selected bacterial isolates possess plant growth-promoting potentials that can make them a potential candidate to be employed as microbial inoculants for protecting plants against phytopathogens, environmental stress and increasing plant growth and productivity.


2019 ◽  
Vol 16 (4) ◽  
pp. 0865 ◽  
Author(s):  
Taha Et al.

In this study, the preparation and characterization of hyacinth plant /chitosan composite, as a heavy metal removal, were done. Water hyacinth plant (Eichhorniacrasspes) was collected from Tigris river in Baghdad. The root and shoot parts of plant were ground to powder. Composite materials were prepared at different ratios of plant part (from 2.9% to 30.3%, wt /wt) which corresponds to (30-500mg) of hyacinth plant (root and shoot) and chitosan. The results showed that all examined ratios of plant parts have an excellent absorption to copper (Cu (II)). Moreover, it was observed that 2.9% corresponds (30mg) of plant root revealed highest removal (82.7%) of Pb (II), while 20.23% of shoot removed 61% of Cd (II) within 24 hr. On the other hand, SEM images displayed a regular shape and high porosity of prepared samples, while FTIR spectra of composite materials showed the characteristic of raw materials bands, which give strong evidence of composite formation.


1991 ◽  
Vol 1066 (2) ◽  
pp. 257-260 ◽  
Author(s):  
Peter Norberg ◽  
Jan-Erik Månsson ◽  
Conny Liljenberg

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