High efficiency transformation of peppermint (Mentha × piperita L.) with Agrobacterium tumefaciens

Plant Science ◽  
1998 ◽  
Vol 136 (1) ◽  
pp. 101-108 ◽  
Author(s):  
F. Diemer ◽  
F. Jullien ◽  
O. Faure ◽  
S. Moja ◽  
M. Colson ◽  
...  
2020 ◽  
pp. 15-20
Author(s):  
Ersin Yucel ◽  
Mine Yucel

In this study, the usage of the peppermint (Mentha piperita) for extracting the metal ions [Mg (II), Cr (II), Ni (II), Cu (II), Zn (II), Cd (II), Pb (II)] that exist at water was investigated. In order to analyze the stability properties, Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherms were used at removing the metal ions and the highest correlation coefficients (R2) were obtained at Langmuir isotherm. Therefore, it is seen that the Langmuir model is more proper than the Freundlich model. However, it was found that the correlation coefficients of removing Ni and Cd is higher at Freundlich model than Langmuir and low at Dubinin-Radushkevich isotherm. It is established that the biosorption amount increase depends on the increase of biosorbent and it can be achieved high efficiency (95%) even with small amount (0.6 mg, peppermint extract) at lead ions. It is also determined that the peppermint extracted that is used at this study shows high biosorption capacity for metal ions and can be used for immobilization of metals from polluted areas.


2021 ◽  
Vol 7 (11) ◽  
pp. 961
Author(s):  
Virginia Casado-del Castillo ◽  
Andrew P. MacCabe ◽  
Margarita Orejas

Protoplast transformation for the introduction of recombinant DNA into Aspergillus nidulans is technically demanding and dependant on the availability and batch variability of commercial enzyme preparations. Given the success of Agrobacterium tumefaciens-mediated transformation (ATMT) in diverse pathogenic fungi, we have adapted this method to facilitate transformation of A. nidulans. Using suitably engineered binary vectors, gene-targeted ATMT of A. nidulans non-homologous end-joining (NHEJ) mutant conidia has been carried out for the first time by complementation of a nutritional requirement (uridine/uracil auxotrophy). Site-specific integration in the ΔnkuA host genome occurred at high efficiency. Unlike other transformation techniques, however, cross-feeding of certain nutritional requirements from the bacterium to the fungus was found to occur, thus limiting the choice of auxotrophies available for ATMT. In complementation tests and also for comparative purposes, integration of recombinant cassettes at a specific locus could provide a means to reduce the influence of position effects (chromatin structure) on transgene expression. In this regard, targeted disruption of the wA locus permitted visual identification of transformants carrying site-specific integration events by conidial colour (white), even when auxotrophy selection was compromised due to cross-feeding. The protocol described offers an attractive alternative to the protoplast procedure for obtaining locus-targeted A. nidulans transformants.


2001 ◽  
Vol 20 (2) ◽  
pp. 150-156 ◽  
Author(s):  
T. Johnson ◽  
D. A. Somers ◽  
A. Das ◽  
J. Ke ◽  
R. Khan

1998 ◽  
Vol 17 (3) ◽  
pp. 165-171 ◽  
Author(s):  
X. Niu ◽  
K. Lin ◽  
P. M. Hasegawa ◽  
R. A. Bressan ◽  
S. C. Weller

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