Plant Regeneration from Pea Leaflets Cultured in vitro and Genetic Stability of Regenerants

1984 ◽  
Vol 117 (2) ◽  
pp. 119-130 ◽  
Author(s):  
A. Rubluo ◽  
K.K. Kartha ◽  
L.A. Mroginski ◽  
J. Dyck
Keyword(s):  
Plant Regeneration ◽  
Genetic Stability

Effects of low temperature on Chrysanthemum shiwogiku var. kinokuniense in vitro conservation

2018 ◽  
Vol 44 (4) ◽  
pp. 675-678
Author(s):  
Weilan ◽  
Shucheng Xu ◽  
Xuelan Zhu
Keyword(s):  
Plant Regeneration ◽  
Low Temperature ◽  
Genetic Stability ◽  
Test Tube ◽  
In Vitro Conservation ◽  
Young Plant ◽  
In Vitro Plantlets ◽  
Surviving Rate ◽  
Issr Pcr

The effect of low temperature condition on wild Chrysanthemum shiwogiku var. in vitro conservation was investigated, and its plant regeneration hereditary stability was detected using its sterile seedling. The results show that the test tube young plant grows rapidly, and its preservation time is short at a temperature at 25±2°C, at condition of 12 h/d, photoperiod at 2000 ~ 3000 lx, and all die after 180 days. However, the sterile seedling grows slowly under low-temperature, and its preservation time extends to 360 days. The sterile seedling surviving rate was above 96%. The data indicate that the 4°C low temperature is advantageous to Chrysanthemum plantlets preservation. After preservation, the recovered plantlets grow well and show no differences in morphology and isoenzyme zymogram of peroxidase, ISSR-PCR compared with the control. In addition, the results show that low temperature in vitro plantlets maintain genetic stability.

scholarly journals Plant regeneration from hypocotyls of black carrot via direct somatic embryogenesis and determination of its genetic stability by RAPD and iPBS methods

2018 ◽  
Vol 78 (3) ◽  
Author(s):  
Burcu Çetin
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Genetic Stability ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Conservation ◽  
Direct Somatic Embryogenesis ◽  
Ms Medium ◽  
Black Carrot

A study was carried out to investigate plant regeneration from hypocotyls of black carrot (Daucus carota spp. sativus), an industrially and medicinally important plant via somatic embryogenesis, and the genetic stability of obtained plantlets with the random amplified polymorphic DNA (RAPD) and inter primer binding sites (iPBS) methods. Hypocotyl explants isolated from in vitro germinated seeds were incubated initially at Murashige and Skoog (MS) medium containing 0.5 mg/L or 1 mg/L 2,4-D for 2 weeks and then at hormone-free MS medium in order to obtain somatic embryos, which were observed after 15 days on the explants. Performed molecular analyses showed that all bands obtained from plantlets through micropropagation were monomorphic. This protocol where black carrot was determined to be clonally reproduced through somatic embryogenesis may lead the way for further research regarding germplasm conservation and breeding studies.

In vitro plant regeneration of caper (Capparis spinosa L.) from floral explants and genetic stability of regenerants

2011 ◽  
Vol 109 (2) ◽  
pp. 373-381 ◽  
Author(s):  
Angela Carra ◽  
Maurizio Sajeva ◽  
Loredana Abbate ◽  
Mirko Siragusa ◽  
Francesco Sottile ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Stability ◽  
In Vitro Plant Regeneration ◽  
Capparis Spinosa ◽  
In Vitro Plant ◽  
Floral Explants

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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