Basic fibroblast growth factor corrects proliferative derangement of bone marrow stroma and CD34+ population following allogeneic stem cell transplantation

Abstract Background: Gastrointestinal tract function and it's integrity are controlled by a number of peptides whose secretion is influenced by severe inflammation. In stomach the main regulatory peptide is ghrelin. For upper small intestine cholecystokinin and lower small intestine glucagon-like peptide- 1 are secreted, while fibroblast growth factor-21 is secreted by several organs, including the liver, pancreas, and adipose tissue [12]. Hematopoietic stem cell transplantation causes serious mucosal damage, which can reflect on this peptides. Methods: The aim of the study was to determine fasting plasma concentrations of ghrelin, cholecystokinin, glucagon- like peptide-1, and fibroblast growth factor -21, and their gene expressions, before and 6 months after hematopoietic stem cell transplantation. 27 children were studied, c ontrol group included 26 healthy children. Results: Acute graft versus host disease was diagnosed in 11 patients (41%, n=27). Median pre-transplantation concentrations of gastrointestinal peptides , as well as their gene expressions, were significantly lower in studied group compared with the control group. Only median of fibroblast growth factor-21 concentration was near-significantly higher before stem cell transplantation than in the control group. The post–hematopoietic transplant results revealed significantly higher concentrations of the studied peptides (except fibroblast growth factor-21) and respective gene expressions as compare to pre transplant results . M edian glucagone like peptide-1 concentrations were significantly decreased in patients with features of acute graft versus host disease . Moreover, negative correlation between glucagone like peptide-1 concentrations and acute graft versus host disease severity was found. Conclusions: Increased concentrations and gene expressions of gastrointestinal tract regulation peptides can be caused by stimulation of regeneration in the severe injured organ . Measurement of these parameters may be a useful method of assessment of severity of gastrointestinal tract complications of hematopoietic stem cell transplantation.

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Basic fibroblast growth factor (bFGF) and its receptor expression (bek and flg) In bone marrow stroma of murine AIDS

Virus Research ◽

10.1016/j.virusres.2004.01.007 ◽

2004 ◽

Vol 101 (2) ◽

pp. 175-184 ◽

Cited By ~ 2

Author(s):

Kam-Fai Tse ◽

Mohammed S Inayat ◽

Jennifer K Morrow ◽

Richard DellaPuca ◽

Nedda K Hughes ◽

...

Keyword(s):

Bone Marrow ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Receptor Expression ◽

Bone Marrow Stroma ◽

Fibroblast Growth ◽

Murine Aids ◽

Marrow Stroma

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Rapid Regression of Bone Marrow Fibrosis after Dose-Reduced Allogeneic Stem Cell Transplantation in Patients with Myelofibrosis.

Blood ◽

10.1182/blood.v108.11.374.374 ◽

2006 ◽

Vol 108 (11) ◽

pp. 374-374

Author(s):

Nicolaus Kroeger ◽

Juergen Thiele ◽

Axel Zander ◽

Michael Kvasnicka

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Growth Factor ◽

Stem Cell Transplantation ◽

Cell Transplantation ◽

Allogeneic Stem Cell Transplantation ◽

Bone Marrow Fibrosis ◽

Myeloid Metaplasia ◽

Bone Marrow Histology ◽

Marrow Fibrosis

Abstract Myelofibrosis with myeloid metaplasia [MMM] is a myeloproliferative disease of which bone marrow histology is characterized by substantial collagen fibrosis and osteosclerosis. Bone marrow histology changes in MMM are reactive and cytokine mediated. Studies suggested a pathogenic role of transforming growth factor β [TGF-β], platelet derived growth factor, and basic fibroblast growth factor. In the current study we investigated the dynamic and kinetic of fibrosis regression in 18 patients with myelofibrosis who underwent allogeneic stem cell transplantation after a dose-reduced conditioning. The conditioning regimen consisted of Busulfan 10 mg/kg, Fludarabin 180 mg/m2, and Antithymocyte globulin 30 – 60 mg/kg followed by peripheral stem cell transplantation (SCT). 18 patients with a median age of 45 [range 35 to 64] were included and bone marrow histology was investigated prior SCT, between day+30 and +40 after engraftment, on day+100, and 1 year after stem cell transplantation. The grading of myelofibrosis [MF] was performed according to the recently published European Consensus on Grading Bone Marrow Fibrosis [Thiele et al., Haematologica2005;90:1125–1132]. This consensus distinguishes between: MF - 0 Scattered linear reticulin with no intersections [cross-overs] corresponding to normal bone marrow MF - 1 Loose network of reticulin with many intersections, especially in perivascular areas MF - 2 Diffuse and dense increase in reticulin with extensive intersections, occasionally with only focal bundles of collagen and/or focal osteosclerosis MF - 3 Diffuse and dense increase in reticulin with extensive intersections with coarse bundles of collagen, often associated with significant osteosclerosis At time of transplantation all patients suffered from advanced myelofibrosis 2° [39 %] or 3° [61 %], resulting in a median MF grade of 3 [range 2–3]. After engraftment on day+30 a regression of MF grade to a median of 2 [range 0–3] was observed. Grade 2 or less was observed in 80 % and grade 1 or 0 in 40 % of the patients. On day+100 the median grade of MF was 1 [range 0–3] and 93 % of the patients had an MF-grade of 2 or less, while 73 % of the patients had MF grade-1 or 0. One year after transplantation the median MF-grade was 0 [range 0–2] and 100 % of the patients had a MF-grade of 2 or less and 92 % of the patients had MF grade 1 or 0. We conclude that allogeneic stem cell transplantation after a dose-reduced conditioning induces a rapid and nearly complete regression of bone marrow fibrosis within the first year after transplantation.

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Peptides regulating gastrointestinal function in children before and after hematopoietic stem cell transplantation

10.21203/rs.2.16711/v1 ◽

2019 ◽

Author(s):

Magdalena Rej ◽

Szymon Skoczeń ◽

Danuta Pietrys ◽

Kinga Kwiecińska ◽

Przemysław J. Tomasik ◽

...

Keyword(s):

Stem Cell ◽

Growth Factor ◽

Hematopoietic Stem Cell Transplantation ◽

Stem Cell Transplantation ◽

Fibroblast Growth Factor ◽

Cell Transplantation ◽

Fibroblast Growth Factor 21 ◽

Fibroblast Growth ◽

Gene Expressions ◽

Hematopoietic Stem

Abstract Gastrointestinal tract function and it's integrity are controlled by a number of peptides whose secretion is influenced by severe inflammation. In stomach the main regulatory peptide is ghrelin. For upper small intestine cholecystokinin and glucagon-like peptide- 1 are secreted, while fibroblast growth factor-21 is secreted by several organs, including the liver, pancreas, and adipose tissue [12]. Hematopoietic stem cell transplantation causes serious mucosal damage, which can reflect on this peptides. The aim of the study was to determine fasting plasma concentrations of ghrelin, cholecystokinin, glucagone- like peptide-1, and fibroblast growth factor -21, and their gene expressions, before and 6 months after hematopoietic stem cell transplantation. 27 children were studied. C ontrol group included 26 healthy children. Acute graft versus host disease was diagnosed in 11 patients (41%, n=27). Median pre-transplantation concentrations of ghrelin, cholecystokinin , and glucagone like peptide-1, as well as their gene expressions, were significantly lower compared with the control group. In contrast, median fibroblast growth factor-21 concentrations were near-significantly higher before stem cell transplantation than in the control group. A comparison of pre- and post-transplantation groups revealed significantly higher concentrations of the studied peptides (except fibroblast growth factor-21) and respective gene expressions in the post–hematopoietic transplant group. M edian glucagone like peptide-1 concentrations were significantly decreased in patients with features of acute graft versus host disease. Moreover, negative correlation between glucagone like peptide-1 concentrations and acute graft versus host disease severity was found. Increased concentrations and gene expressions of gastrointestinal tract regulation peptides can be caused by stimulation of regeneration in the severe injured organ. Measurement of these parameters may be a useful method of assessment of severity of gastrointestinal tract complications of hematopoietic stem cell transplantation.

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Gastrointestinal peptides in children before and after hematopoietic stem cell transplantation

10.21203/rs.2.16711/v3 ◽

2020 ◽

Author(s):

Szymon Skoczeń ◽

Magdalena Rej ◽

Danuta Pietrys ◽

Kinga Kwiecińska ◽

Przemysław J. Tomasik ◽

...

Keyword(s):

Stem Cell ◽

Growth Factor ◽

Hematopoietic Stem Cell Transplantation ◽

Stem Cell Transplantation ◽

Fibroblast Growth Factor ◽

Cell Transplantation ◽

Fibroblast Growth Factor 21 ◽

Fibroblast Growth ◽

Gene Expressions ◽

Hematopoietic Stem

Abstract Background: Gastrointestinal tract function and it's integrity are controlled by a number of peptides whose secretion is influenced by severe inflammation. In stomach the main regulatory peptide is ghrelin. For upper small intestine cholecystokinin and lower small intestine glucagon-like peptide- 1 are secreted, while fibroblast growth factor-21 is secreted by several organs, including the liver, pancreas, and adipose tissue [12]. Hematopoietic stem cell transplantation causes serious mucosal damage, which can reflect on this peptides. Methods: The aim of the study was to determine fasting plasma concentrations of ghrelin, cholecystokinin, glucagon- like peptide-1, and fibroblast growth factor-21, and their gene expressions, before and 6 months after hematopoietic stem cell transplantation.27 children were studied, control group included 26 healthy children. Results: Acute graft versus host disease was diagnosed in 11 patients (41%, n=27). Median pre-transplantation concentrations of gastrointestinal peptides, as well as their gene expressions, were significantly lower in studied group compared with the control group. Only median of fibroblast growth factor-21 concentration was near-significantly higher before stem cell transplantation than in the control group. The post–hematopoietic transplant results revealed significantly higher concentrations of the studied peptides (except fibroblast growth factor-21) and respective gene expressions as compare to pre transplant results. Median glucagone like peptide-1 concentrations were significantly decreased in patients with features of acute graft versus host disease. Moreover, negative correlation between glucagone like peptide-1 concentrations and acute graft versus host disease severity was found. Conclusions: Increased concentrations and gene expressions of gastrointestinal tract regulation peptides can be caused by stimulation of regeneration in the severe injured organ. Measurement of these parameters may be a useful method of assessment of severity of gastrointestinal tract complications of hematopoietic stem cell transplantation.

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