Genistein exerts estrogen-like effects in male mouse reproductive tract

By use of the biotin-avidin immunohistochemical method and a homologous antiserum as the primary antiserum, relaxin immunostaining was absent in the testes, prostate, seminal vesicles, and epididymides of the rat. Relaxin immunostaining was also lacking when anti-porcine relaxin serum was employed as the primary antiserum. Furthermore, immunohistochemical studies for relaxin localization in the reproductive tract of the male mouse using both anti-rat and anti-porcine relaxin sera also revealed an absence of the hormone in the reproductive system of this species. Although this study suggests that immunoreactive relaxin is absent in the male reproductive tracts of both the rat and mouse, it raises some questions concerning the reports in the literature of the presence of relaxin-like substances in the male reproductive tracts of other species. These reports are discussed in relation to our current results.

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1395: Presence in Mice and Men of a Novel Class of Leukocyte/Macrophages Essential Fornormal Development of Male Mouse Reproductive Tract

The Journal of Urology ◽

10.1016/s0022-5347(18)38620-8 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 367-367

Author(s):

Robert C. Eyre ◽

Thomas E. Mullen ◽

Rachel L. Kiessling ◽

Ann A. Kiessling

Keyword(s):

Male Mouse ◽

Reproductive Tract

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Expression patterns of activin, inhibin and follistatin variants in the adult male mouse reproductive tract suggest important roles in the epididymis and vas deferens

Reproduction Fertility and Development ◽

10.1071/rd11287 ◽

2013 ◽

Vol 25 (3) ◽

pp. 570 ◽

Cited By ~ 21

Author(s):

Wendy R. Winnall ◽

Hui Wu ◽

Mai A. Sarraj ◽

Peter A. W. Rogers ◽

David M. de Kretser ◽

...

Keyword(s):

Male Mouse ◽

Reproductive Tract ◽

Vas Deferens ◽

Expression Patterns ◽

Activin A ◽

Gene Encoding ◽

Male Reproductive Tract ◽

Α Subunit ◽

Protein Levels ◽

Specific Regulation

Activin A and its inhibitors follistatin and inhibin play key roles in development and function of the male reproductive tract. Quantitative (q) polymerase chain reaction (PCR) was used to evaluate the expression of Inhba (the gene encoding activin A subunits), Inha and Inhbb (genes encoding the inhibin B subunits), as well as the genes for follistatin (Fst) and follistatin-like 3 (Fstl3) and the activin receptor subunits, in the male mouse reproductive tract. A qPCR assay that discriminated between the two follistatin variants of Fst288 (tissue-bound form) and Fst315 (circulating) was established. Activin A protein was measured by ELISA, whereas the inhibin α-subunit and total follistatin proteins were measured by radioimmunoassay (RIA). A screen of 22 tissues demonstrated tissue-specific regulation of the follistatin variants, with Fst288 highly expressed in the vas deferens and Fst315 most highly expressed in the skin. The expression of Fst288 and Fst315 and follistatin protein levels increased progressively from the testis through to the distal vas deferens. Inhba and the activin receptors were highly expressed in the epididymis, but activin A protein was elevated in both the epididymis and vas deferens. Inhibin α-subunit mRNA and protein and Inhbb expression were highest in the testis. These results indicate a role for activin A within the epididymis, but also that activin A bioactivity may be increasingly inhibited by follistatin distally along the male reproductive tract.

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THE EFFECT OF TESTOSTERONE AND PROGESTERONE ON THE RESPONSE OF THE RETICULO-ENDOTHELIAL SYSTEM AND REPRODUCTIVE TRACT TO OESTROGEN IN THE MALE MOUSE

Journal of Endocrinology ◽

10.1677/joe.0.0370017 ◽

1967 ◽

Vol 37 (1) ◽

pp. 17-21 ◽

Cited By ~ 4

Author(s):

T. NICOL ◽

B. VERNON-ROBERTS ◽

D. C. QUANTOCK

Keyword(s):

Male Mouse ◽

Reproductive Tract ◽

Biological Activities ◽

Levator Ani ◽

Weight Increase ◽

Stimulant Effect ◽

Reticulo Endothelial System

SUMMARY In intact and orchidectomized animals testosterone had no effect on the reticulo-endothelial system (RES) when administered alone; it potentiated the stimulant effect of 17β-oestradiol on the RES, but had no effect on the RE stimulation produced by diethylstilboestrol. Progesterone was a mild RE stimulant; it had no effect on the RE stimulation produced by 17β-oestradiol or diethylstilboestrol. Both testosterone and oestrogen caused weight increase in the levator ani of orchidectomized animals; progesterone had no effect. Testosterone and progesterone had no effect on the myotrophic action of 17β-oestradiol, but both compounds inhibited the myotrophic effect of diethylstilboestrol. The results show that in the male, as in the female, the action of oestrogen on the RES is independent of its action on the reproductive tract, although these two biological activities are contained in the same molecule.

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Different Effects of Androgen on the Expression of Fut1, Fut2, Fut4 and Fut9 in Male Mouse Reproductive Tract

International Journal of Molecular Sciences ◽

10.3390/ijms141123188 ◽

2013 ◽

Vol 14 (11) ◽

pp. 23188-23202 ◽

Cited By ~ 2

Author(s):

Chun-Mei Wang ◽

Shuang-Gang Hu ◽

Yan-Fei Ru ◽

Guang-Xin Yao ◽

Wu-Bin Ma ◽

...

Keyword(s):

Male Mouse ◽

Reproductive Tract

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Distribution of Vitamin D Receptor and 1α-Hydroxylase in Male Mouse Reproductive Tract

Reproductive Sciences ◽

10.1177/1933719112459235 ◽

2012 ◽

Vol 20 (4) ◽

pp. 426-436 ◽

Cited By ~ 28

Author(s):

Ahmad Reza Mahmoudi ◽

Amir Hassan Zarnani ◽

Mahmood Jeddi-Tehrani ◽

Leila Katouzian ◽

Maryam Tavakoli ◽

...

Keyword(s):

Vitamin D ◽

Vitamin D Receptor ◽

Male Mouse ◽

Reproductive Tract

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Immunohistochemical Localization of Microsomal PGE Synthase-1 and Cyclooxygenases in Male Mouse Reproductive Organs

Endocrinology ◽

10.1210/endo.143.6.8872 ◽

2002 ◽

Vol 143 (6) ◽

pp. 2410-2419 ◽

Cited By ~ 33

Author(s):

Michael Lazarus ◽

Craig J. Munday ◽

Naomi Eguchi ◽

Shigeko Matsumoto ◽

Gary J. Killian ◽

...

Keyword(s):

Epithelial Cells ◽

Male Mouse ◽

Cellular Localization ◽

Reproductive Tract ◽

Vas Deferens ◽

Reproductive Organs ◽

Progressive Increase ◽

Northern Blotting ◽

Cox 2 ◽

Cox 1

Abstract We investigated the tissue distribution and cellular localization of microsomal PGE synthase-1 (mPGES-1) and cyclooxygenase (COX)-1 and -2 in the male mouse reproductive organs. Northern blotting revealed that the mPGES-1 mRNA was expressed intensely in the epididymis and weakly in the lung, spleen, skin, kidney, colon, and brain. In the male reproductive tract, the expression of mPGES-1 increased from the testis to the cauda epididymis and was highest in the vas deferens when examined by Northern blotting, RT-PCR, and Western blotting. By immunohistochemistry, mPGES-1 was detected in Leydig cells of the testis and in epithelial cells of the epididymis, vas deferens, and seminal vesicles. In addition, the caput and cauda regions of the epididymis and the vas deferens in this order showed a progressive increase in the expression of COX-1 mRNA and immunoreactivity, whereas COX-2 was dominantly expressed in the vas deferens. COX-1 was localized in epithelial cells of the caput, corpus and cauda epididymis and of the vas deferens, and COX-2 was evident in epithelial cells of the distal cauda epididymis and vas deferens. These results show that mPGES-1 is expressed coordinately with COX-1 and COX-2 and is involved in PGE2 production in male genital organs.

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