Comparison of indirect immunofluorescent antibody test and modified direct agglutination test methods for detection of Toxoplasma gondii antibodies in adult sheep in Spain

1996 ◽  
Vol 67 (1-2) ◽  
pp. 99-103 ◽  
Author(s):  
M.C. Marca ◽  
J.J. Ramos ◽  
A. Loste ◽  
T. Sáez ◽  
M.C. Sanz
Keyword(s):  
Toxoplasma Gondii ◽  
Antibody Test ◽  
Test Methods ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Adult Sheep ◽  
Immunofluorescent Antibody ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

scholarly journals Toxoplasma gondii antibodies on domiciled cats from Lages municipality, Santa Catarina State, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 268-269 ◽  
Author(s):  
Luciana Dalla Rosa ◽  
Anderson Barbosa de Moura ◽  
Natascha Trevisani ◽  
Alessandra Pereira Medeiros ◽  
Amélia Aparecida Sartor ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Antibody Test ◽  
Southern Brazil ◽  
Santa Catarina ◽  
Factors Associated ◽  
Immunofluorescent Antibody ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

Sera were collected from 300 domiciled cats from the municipality of Lages, Southern Brazil, to determine the prevalence of Toxoplasma gondii antibodies and risk factors associated. Tests for T. gondii antibodies were performed using indirect immunofluorescent antibody test (IFAT). Positive reactions with titers >1:64 were found in 43 (14.33%) cats. A significant number of seropositive cats were >6 month old (p = 0.03758) and had access to the streets or/and rural areas (p = 0.04185). The results indicate that T. gondii is widespread in cats in Lages with a prevalence of 14.33%.

scholarly journals Occurrence of antibodies anti-Toxoplasma gondii among sheltered and free-roaming cats within a university campus

2021 ◽  
Vol 42 (3Supl1) ◽  
pp. 1685-1694
Author(s):  
Louise Bach Kmetiuk ◽  
◽  
Maysa Pellizzaro ◽  
Ana Carolina Yamakawa ◽  
Juliana Cristina Rebonato Mendes ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Environmental Exposure ◽  
Antibody Test ◽  
Skin Lesions ◽  
Prey Consumption ◽  
University Campus ◽  
Immunofluorescent Antibody ◽  
Indirect Immunofluorescent ◽  
Infected Prey ◽  
Immunofluorescent Antibody Test

The present study aimed to assess anti-T. gondii antibodies in sheltered and free-roaming cats within a university campus that has an overlapping population of humans and livestock. A total of 51 cats were tested for anti-T. gondii antibodies using the indirect immunofluorescent antibody test. Overall, 8/51 cats (15.7%) were seropositive. Cats were more likely to be seropositive when free-roaming (p= 0.008) and with presence of skin lesions (p= 0.042), and less likely with < 1 year of age (p= 0.021), probably due to higher environmental exposure and infected prey consumption. The presence of seropositive free-roaming cats whose areas overlapped those occupied by humans and livestock may suggest an increased on-campus chance of T. gondii occurrence.

scholarly journals Occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in cats with outdoor access in São Luís, Maranhão, Brazil

2012 ◽  
Vol 21 (2) ◽  
pp. 107-111 ◽  
Author(s):  
Maria do Socorro Costa de Oliveira Braga ◽  
Marcos Rogério André ◽  
Márcia Mariza Gomes Jusi ◽  
Carla Roberta Freschi ◽  
Márcia Cristina Alves Teixeira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
The Other ◽  
Immunofluorescent Antibody ◽  
Other Hand ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

The present study aimed to investigate the frequency of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in cats with outdoor access in São Luís, Maranhão, Brazil. The presence of IgG anti-T. gondii and anti-N. caninum antibodies was tested using the Indirect Immunofluorescent Antibody Test (IFAT). IgG anti-T. gondii and anti-N. caninum antibodies were detected in 101 (50.5%) and 54 (27%) sampled cats, respectively. The titers of anti-T. gondii antibodies ranged from 40 (cut-off) to 2560. On the other hand, the titers of anti-N. caninum antibodies ranged from 25 (cut-off) to 400. Twenty-seven cats (13.5%) were shown to be seropositive for both parasites. Seventy-four cats (34%) were seropositive only for T. gondii. Twenty-two cats (11%) were seropositive only for N. caninum. The present study showed that cats with outdoor access in São Luís, Maranhão, are exposed to T. gondii and N. caninum.

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

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