Frequency of Pseudomonas aeruginosa serotypes in burn wound infections and their resistance to antibiotics

Introduction/Aim. Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of wound infections, following the disruption of the skin or mucous membranes integrity. The aim of this study was to analyze of the presence P. aeruginosa in wound swabs, antibiotics susceptibility testing, determination of the minimum inhibitory concentrations (MICs) of antibiotics, testing of the metallo-?-lactamases (MBLs) production, isolates serotyping and analysis of the most common serotypes resistance. Methods. A total of 90 outpatients and 55 intpatients wound swabs were cultivated. Wound swabs were taken from the patients with wound infections symptoms. Antibiotics susceptibility testing was performed to: meropenem, imipenem, piperacillin-tazobactam, ceftazidime, cefepime, amikacin, gentamicin, netilmicin, ofloxacin, ciprofloxacin and colistin (HiMedia). Polyvalent and monovalent antisera for agglutination (Biorad) were used in P. aeruginosa agglutination. Results. P. aeruginosa was isolated from 36.55% wound swabs (36.66% of the inpatients wounds and 36.36% of the outpatients). The analyzed isolates showed the highest degree of sensitivity to colistin (100%) and meropenem (93.44%) and the lowest to cefepime (19.54%). The majority of the inpatients isolates had 12 ?g/mL (28.57%) MIC for piperacillin-tazobactam and 16 ?g/mL (28.57%) for the outpatients. The most common MICs for ciprofloxacin were 0.19 ?g/mL (31.81%) for the nosocomial isolates, and 0.25 ?g/mL (28.57%) for the outpatients? ones. The most common MICs for amikacin of the nosocomial isolates were 6 ?g/ml (40.9%), and for the outpatients ones 4 ?g/mL (33.33%). Five (9.43%) isolates produced MBLs. The most common serotypes were P11 (22.64%), P6 (15.09%) and P1 (11.32%). Conclusion. Neither the increased presence of P. aeruginosa was noticed in wounds swabs, nor the antibiotic resistance in the nosocomial isolates compared to those from outpatients. The analyzed isolates had the higest sensitivity to colistin and meropenem, and the lowest to cefepime.

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Effects of phosphate supplementation on Pseudomonas aeruginosa invasive behavior in burn wound infections: A simple approach to a big problem

Burns ◽

10.1016/j.burns.2015.09.003 ◽

2016 ◽

Vol 42 (2) ◽

pp. 428-433 ◽

Cited By ~ 3

Author(s):

Soliman Mohammadi-Samani ◽

Shahriyar Kouroshfard ◽

Negar Azarpira

Keyword(s):

Pseudomonas Aeruginosa ◽

Simple Approach ◽

Wound Infections ◽

Burn Wound ◽

Invasive Behavior ◽

Phosphate Supplementation

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Response to “Burn wound infections and Pseudomonas aeruginosa”

Burns ◽

10.1016/j.burns.2020.02.019 ◽

2020 ◽

Author(s):

C. Jaloux ◽

F. Amatore ◽

N. Macagno ◽

A. Morand ◽

D. Casanova

Keyword(s):

Pseudomonas Aeruginosa ◽

Wound Infections ◽

Burn Wound

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Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections

PLoS ONE ◽

10.1371/journal.pone.0164622 ◽

2016 ◽

Vol 11 (10) ◽

pp. e0164622 ◽

Cited By ~ 23

Author(s):

Maryam Banar ◽

Mohammad Emaneini ◽

Mhboubeh Satarzadeh ◽

Nafiseh Abdellahi ◽

Reza Beigverdi ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Wound Infections ◽

Burn Wound ◽

Biofilm Production

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Evaluation of Metallo-β-Lactamase-Production and Carriage of bla-VIM Genes in Pseudomonas aeruginosa Isolated from Burn Wound Infections in Isfahan

Archives of Trauma Research ◽

10.5812/atr.34343 ◽

2016 ◽

Vol 5 (4) ◽

Cited By ~ 7

Author(s):

Mahmood Saffari ◽

Farzaneh Firoozeh ◽

Mohammad Pourbabaee ◽

Mohammad Zibaei

Keyword(s):

Pseudomonas Aeruginosa ◽

Wound Infections ◽

Burn Wound

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Bacteriological profile with antibiotic sensitivity pattern of burn wound infections in a peripheral tertiary care hospital

International Surgery Journal ◽

10.18203/2349-2902.isj20211307 ◽

2021 ◽

Vol 8 (4) ◽

pp. 1253

Author(s):

Arnab Mandal ◽

Swapan Das

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Susceptibility ◽

Tertiary Care ◽

Antibiotic Sensitivity ◽

Specific Pattern ◽

Care Hospital ◽

Wound Infections ◽

Burn Wound ◽

Mortality And Morbidity ◽

Sensitivity Pattern

Background: India, has an estimated burn incidence of 6-7 million annually. Nearly 10% of these are life threatening and require hospitalization, and main cause of mortality and morbidity of these burn patients were wound infection and sepsis after 1st 24 hours. The present study was tried to determine specific pattern of burn wound infections, and antibiotic susceptibility of those isolates.Methods: After matching inclusion and exclusion criteria, total 55 patients were taken for this institution based, prospective observational study. Wound swabs were collected on day 7 and cultured aerobically in MacConkey agar and 5% blood agar and antibiotic susceptibility testing was done on Muller Hinton agar using Kirby-Bauer disc diffusion method.Results: Among study population 69.1% patients were female and majority (29.1%) of the patients belongs to age group from 21 to 30. It was found that 48 wound swabs were positive for microorganisms, of which Pseudomonas aeruginosa was most common isolated organism (23.6%), followed by Klebsiella pneumoniae (16.4%) and Staphylococcus aureus (14.5%). The most effective antibiotic found in this study was piperacillin/tazobactam, followed by imipenem/cilastatin.Conclusions: It was seen that gram-negative organisms were more prevalent. Pseudomonas aeruginosa was the most common microorganism and piperacillin/tazobactam was most effective antibiotic.

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