Synergistic Action of Silver Sulfadiazine and Sodium Piperacillin on Resistant Pseudomonas aeruginosa in vitro and in Experimental Burn Wound Infections

ABSTRACT Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections. Burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. Infections by notorious pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii impair patient recovery and can even lead to fatality. In this study, we investigated the effect of burn wound exudates (BWEs) on the virulence of those pathogens. BWEs were collected within 7 days after burn trauma from 5 burn patients. We first monitored their effect on pathogen growth. In contrast to A. baumannii and S. aureus, P. aeruginosa was the only pathogen able to grow within these human fluids. Expression of typical virulence factors such as pyocyanin and pyoverdine was even enhanced compared the levels seen with standard laboratory medium. A detailed chemical composition analysis of BWE was performed, which enabled us to determine the major components of BWE and underline the metabolic modifications induced by burn trauma. These data are essential for the development of an artificial medium mimicking the burn wound environment and the establishment of an in vitro system to analyze the initial steps of burn wound infections. IMPORTANCE Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections.

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Aloe vera Gel: Effective Therapeutic Agent against Multidrug-Resistant Pseudomonas aeruginosa Isolates Recovered from Burn Wound Infections

Chemotherapy Research and Practice ◽

10.1155/2015/639806 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 9

Author(s):

Mehdi Goudarzi ◽

Maryam Fazeli ◽

Mehdi Azad ◽

Sima Sadat Seyedjavadi ◽

Reza Mousavi

Keyword(s):

Pseudomonas Aeruginosa ◽

Therapeutic Agent ◽

Biological Activities ◽

Aloe Vera ◽

Multidrug Resistant ◽

Wound Infections ◽

Burn Wound ◽

Aloe Vera Gel ◽

Herbal Medicinal

Objective. Aloe vera is an herbal medicinal plant with biological activities, such as antimicrobial, anticancer, anti-inflammatory, and antidiabetic ones, and immunomodulatory properties. The purpose of this study was investigation of in vitro antimicrobial activity of A. vera gel against multidrug-resistant (MDR) Pseudomonas aeruginosa isolated from patients with burn wound infections. Methods. During a 6-month study, 140 clinical isolates of P. aeruginosa were collected from patients admitted to the burn wards of a hospital in Tehran, Iran. Antimicrobial susceptibility test was carried out against the pathogens using the A. vera gel and antibiotics (imipenem, gentamicin, and ciprofloxacin). Results. The antibiogram revealed that 47 (33.6%) of all isolates were MDR P. aeruginosa. The extract isolated from A. vera has antibacterial activity against all of isolates. Also, 42 (89.4%) isolates were inhibited by A. vera gel extract at minimum inhibitory concentration (MIC) ≤ 200 µg/mL. MIC value of A. vera gel for other isolates (10.6%) was 800 µg/mL. All of MDR P. aeruginosa strains were inhibited by A. vera at similar MIC50 and MIC90 200 µg/mL. Conclusion. Based on our results, A. vera gel at various concentrations can be used as an effective antibacterial agent in order to prevent wound infection caused by P. aeruginosa.

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Enhanced Efficacy of Some Antibiotics in Presence of Silver Nanoparticles Against Multidrug Resistant Pseudomonas aeruginosa Recovered From Burn Wound Infections

Frontiers in Microbiology ◽

10.3389/fmicb.2021.648560 ◽

2021 ◽

Vol 12 ◽

Author(s):

Maha A. Khalil ◽

Gamal M. El Maghraby ◽

Fatma I. Sonbol ◽

Nanis G. Allam ◽

Perihan S. Ateya ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Silver Nanoparticles ◽

Pharmaceutical Formulations ◽

Inhibition Zone ◽

Multidrug Resistant ◽

Wound Infections ◽

Burn Wound ◽

Ag Nps

Burn wound infections with multidrug-resistant (MDR) bacteria are shown in many countries as severe widespread health threats. Consequently, attention has been devoted to new nanoparticle-based materials in the field of antimicrobial chemotherapy for burn wound infections. This study aimed to evaluate both in vitro and in vivo efficacies of nanoparticle–antibiotic combinations as new classes of materials subjected against MDR Pseudomonas aeruginosa. Out of 40 Gram-negative isolates, 23 P. aeruginosa were recovered from patients with burn wound infections attending different hospitals in Tanta, Egypt. The susceptibility test revealed that 95.7% of P. aeruginosa isolates were MDR with a high incidence of resistance against carbenicillin. Antibacterial activities of silver nanoparticles (Ag-NPs) against the isolates examined showed various inhibition zone diameters ranging from 11 to 17 mm. Strong synergistic efficacy of neomycin was reported in combination with Ag-NPs against MDR P. aeruginosa P8 and P14 isolates. The in vivo effectiveness of various pharmaceutical formulations prepared from a combination of neomycin antibiotic with Ag-NPs in the treatment of induced bacterially infected mice burns showed that maximum healing activity along with faster wound contraction reported with the combination of neomycin-Ag-NPs in the spray formulation. Generally, data indicated that incorporating Ag-NPs in combination with certain antibiotics may be a new, promising application for wound treatments, especially burns infected with MDR P. aeruginosa.

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Effects of phosphate supplementation on Pseudomonas aeruginosa invasive behavior in burn wound infections: A simple approach to a big problem

Burns ◽

10.1016/j.burns.2015.09.003 ◽

2016 ◽

Vol 42 (2) ◽

pp. 428-433 ◽

Cited By ~ 3

Author(s):

Soliman Mohammadi-Samani ◽

Shahriyar Kouroshfard ◽

Negar Azarpira

Keyword(s):

Pseudomonas Aeruginosa ◽

Simple Approach ◽

Wound Infections ◽

Burn Wound ◽

Invasive Behavior ◽

Phosphate Supplementation

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Study on the Mechanism of Application of Eucalyptus Volatile oil in Prevention and Treatment of Burn Wound Infections in Vitro

10.21203/rs.3.rs-19542/v1 ◽

2020 ◽

Author(s):

Lei Yang ◽

Qing Huang ◽

Xingxin Gao ◽

Zhimin Lin ◽

Songlin Chen ◽

...

Keyword(s):

Volatile Oil ◽

Bacterial Biofilm ◽

Control Group ◽

Wound Infections ◽

Eucalyptus Urophylla ◽

Burn Wound ◽

Working Mechanism ◽

Biofilm Model ◽

Prevention And Treatment

Abstract Objective To investigate working mechanism of Eucalyptus volatile oil on the prevention and treatment of burn wound infections. Methods P.a biofilm model was used to investigate the effect of Eucalyptus volatile oil on bacterial biofilm. The expression of LasI mRNA in P.a was detected by RT-PCR. Results MIC test showed that the volatile oil of Eucalyptus Urophylla in a concentration of 20% or more could exert anti-bacterial effect. However, no zone of inhibition could be observed in the neither high nor low concentration of the Eucalyptus volatile oil. Scanning electron microscopy results showed a significant delay in volatile oil groups when compared with the control group. The expression of LasI mRNA in the volatile oil group was significantly lower than that in the control group. Conclusion As Chinese medicine, the volatile oil of Eucalyptus Urophylla can affect the proliferation of P.a and biofilm formation by interfering with the expression of LasI, thus achieving the purpose of preventing and treating infection of burn patients.

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Response to “Burn wound infections and Pseudomonas aeruginosa”

Burns ◽

10.1016/j.burns.2020.02.019 ◽

2020 ◽

Author(s):

C. Jaloux ◽

F. Amatore ◽

N. Macagno ◽

A. Morand ◽

D. Casanova

Keyword(s):

Pseudomonas Aeruginosa ◽

Wound Infections ◽

Burn Wound

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Dealing with biofilms of Pseudomonas aeruginosa and Staphylococcus aureus: In vitro evaluation of a novel aerosol formulation of silver sulfadiazine

Burns ◽

10.1016/j.burns.2019.07.027 ◽

2020 ◽

Vol 46 (1) ◽

pp. 128-135 ◽

Cited By ~ 4

Author(s):

Alejandro J. Moyano ◽

Carlos R. Mas ◽

Claudia A. Colque ◽

Andrea M. Smania

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Silver Sulfadiazine ◽

In Vitro Evaluation ◽

And Staphylococcus Aureus

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A comparative study of the effect of different topical agents on burn wound infections

Indian Journal of Plastic Surgery ◽

10.4103/0970-0358.101320 ◽

2012 ◽

Vol 45 (02) ◽

pp. 374-378 ◽

Cited By ~ 8

Author(s):

Katara Gunjan ◽

Chamania Shobha ◽

Chitnis Sheetal ◽

Hemvani Nanda ◽

Chitnis Vikrant ◽

...

Keyword(s):

Silver Nitrate ◽

Benzalkonium Chloride ◽

Research Centre ◽

Topical Agent ◽

Wound Infections ◽

Burn Wound ◽

Gram Positive ◽

Gram Negative ◽

Excellent Activity

ABSTRACT Background: Topical agents are used to treat burn wound infections. Aims and Objective: The present work was aimed to find out the in vitro efficacy of different topical agents against burn wound pathogens. Settings and Design: Randomly selected gram-positive (29) and gram-negative bacterial (119) isolates from burn wound cases admitted in burn unit of Choithram Hospital and Research Centre, Indore, were included in the in vitro activity testing for silver nitrate, silver sulphadiazine (SSD), chlorhexidine, cetrimide, nitrofuran, soframycin, betadine, benzalkonium chloride and honey by growth inhibition on agar medium. Materials and Methods: Multidrug-resistant isolates of gram-positive and gram-negative bacteria were checked for different topical agents. 1% topical agent was mixed with Mueller-Hinton agar. Two microlitres of bacterial suspension adjusted to 0.5 McFarland turbidity standard was spread over the topical agent containing plates. The plates without the topical agent were used as control plates. The plates were incubated for 48 h at 37΀C. Results: SSD (148/148), silver nitrate (148/148) and chlorhexidine (148/148) showed excellent activity against all the pathogens. Neosporin had poor activity against Pseudomonas aeruginosa, (4/44) Proteus spp. (2/4) and group D streptococci (1/4). Betadine did not show activity against the bacterial isolates in the presence of organic matter. Honey did not exert any antimicrobial activity under the study conditions. Conclusion: SSD, silver nitrate and chlorhexidine have excellent activity against all the bacterial pathogens and could be used empirically, while identification of the infective agent is required for selecting the alternative topical agents such as nitrofuran, soframycin, and benzalkonium chloride.

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Preclinical In Vitro and In Vivo Characterization of the Fully Human Monoclonal IgM Antibody KBPA101 Specific for Pseudomonas aeruginosa Serotype IATS-O11

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01142-09 ◽

2010 ◽

Vol 54 (6) ◽

pp. 2338-2344 ◽

Cited By ~ 35

Author(s):

Michael P. Horn ◽

Adrian W. Zuercher ◽

Martin A. Imboden ◽

Michael P. Rudolf ◽

Hedvika Lazar ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Treatment Options ◽

Human Monoclonal Antibody ◽

Human Monocyte ◽

Peripheral Blood Lymphocytes ◽

Infection Model ◽

High Avidity ◽

Burn Wound

ABSTRACT Pseudomonas aeruginosa infection in ventilator-associated pneumonia is a serious and often life-threatening complication in intensive care unit patients, and new treatment options are needed. We used B-cell-enriched peripheral blood lymphocytes from a volunteer immunized with a P. aeruginosa O-polysaccharide-toxin A conjugate vaccine to generate human hybridoma cell lines producing monoclonal antibodies specific for individual P. aeruginosa lipopolysaccharide serotypes. The fully human monoclonal antibody secreted by one of these lines, KBPA101, is an IgM/κ antibody that binds P. aeruginosa of International Antigenic Typing System (IATS) serotype O11 with high avidity (5.81 × 107 M−1 ± 2.8 × 107 M−1) without cross-reacting with other serotypes. KBPA101 specifically opsonized the P. aeruginosa of IATS O11 serotype and mediated complement-dependent phagocytosis in vitro by the human monocyte-like cell line HL-60 at a very low concentration (half-maximal phagocytosis at 0.16 ng/ml). In vivo evaluation of KBPA101 demonstrated a dose-response relationship for protection against systemic infections in a murine burn wound sepsis model, where 70 to 100% of animals were protected against lethal challenges with P. aeruginosa at doses as low as 5 μg/animal. Furthermore, a high efficacy of KBPA101 in protection from local respiratory infections in an acute lung infection model in mice was demonstrated. Preclinical toxicology evaluation on human tissue, in rabbits, and in mice did not indicate any toxicity of KBPA101. Based on these preclinical findings, the first human clinical trials have been initiated.

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