Cloning and heterologous expression of Entamoeba histolytica adenylate kinase and uridylate/cytidylate kinase

Gene ◽  
1998 ◽  
Vol 209 (1-2) ◽  
pp. 219-228 ◽  
Author(s):  
Lidya B Sánchez ◽  
Miklós Müller
Keyword(s):  
Heterologous Expression ◽  
Entamoeba Histolytica ◽  
Adenylate Kinase ◽  
Cytidylate Kinase

scholarly journals The basic isoform of profilin in pathogenic Entamoeba histolytica. cDNA Cloning, Heterologous Expression, and Actin-Binding Properties

1995 ◽  
Vol 233 (3) ◽  
pp. 976-981 ◽  
Author(s):  
Marina Binder ◽  
Stephan Ortner ◽  
Hertha Erben ◽  
Otto Scheiner ◽  
Gerhard Wiedermann ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Cdna Cloning ◽  
Entamoeba Histolytica ◽  
Actin Binding ◽  
Binding Properties

scholarly journals Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and adenylate kinase

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181370 ◽  
Author(s):  
Charumathi Jayachandran ◽  
Balakumaran Palanisamy Athiyaman ◽  
Meenakshisundaram Sankaranarayanan
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Adenylate Kinase ◽  
Nadh Oxidase ◽  
Cofactor Engineering

scholarly journals Heterologous Expression of an Entamoeba histolytica Chitin Synthase in Saccharomyces cerevisiae

2006 ◽  
Vol 5 (1) ◽  
pp. 203-206 ◽  
Author(s):  
Katrina L. Van Dellen ◽  
Dorota A. Bulik ◽  
Charles A. Specht ◽  
Phillips W. Robbins ◽  
John C. Samuelson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heterologous Expression ◽  
Entamoeba Histolytica ◽  
Cyst Wall ◽  
Lateral Wall ◽  
Chitin Synthase ◽  
Accessory Proteins ◽  
Chitin Synthases

ABSTRACT Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.

Entamoeba histolytica, heterologous expression and in situ immunolocalization of ornithine decarboxylase (EhODC)

2009 ◽  
Vol 123 (1) ◽  
pp. 99-104
Author(s):  
Yolanda Terán-Figueroa ◽  
Pablo Arteaga-Nieto ◽  
America Bethancourt-Rodríguez ◽  
Maria Luisa Labra-Barrios ◽  
Juan Pedro Luna-Arias ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Ornithine Decarboxylase ◽  
Entamoeba Histolytica

Erythrophagocytosis by Entamoeba histolytica.- Ultrastructural Study

1972 ◽  
Vol 30 ◽  
pp. 156-157 ◽  
Author(s):  
Norberto Treviño ◽  
Alfredo Feria-Velasco ◽  
I. Ruiz de Chávez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Entamoeba Histolytica ◽  
Ultrastructural Study ◽  
Saline Solution ◽  
Physiological Saline ◽  
Ultrastructural Level ◽  
Hot Plate ◽  
Physiological Saline Solution ◽  
Axenic Conditions

Although erythrophagocytosis by various species of Entamoeba is a well known phenomenon this has not yet been studied in detail at the ultrastructural level. The present work deals with the description of the incorporation process of erythrocytes by trophozoites of E. histolytica. For this study, trophozoites of E. histolytica, HK-9:NIH strain cultured in axenic conditions and washed human erythrocytes were placed on a hot plate at 37°C in physiological saline solution. After 5 minutes, 2.5% glutarldehyde was added and the samples were processed according to conventional techniques for electron microscopy.Based upon light microscopy studies on living trophozoites in contact with erythrocytes, it seems that erythrophagocytosis only takes place in one pole of the parasite.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Sign in / Sign up

Export Citation Format

Share Document