Cell death in plastic tissue culture flasks induced by fluid shear force

1998 ◽  
Vol 95 ◽  
pp. 193 ◽  
Author(s):  
R. Tchao
Keyword(s):  
Tissue Culture ◽  
Cell Death ◽  
Shear Force ◽  
Fluid Shear ◽  
Plastic Tissue Culture

Plastic Tissue-Culture Dishes in Diagnostic Mycology

1964 ◽  
Vol 41 (4) ◽  
pp. 385-387
Author(s):  
R. C. Reynolds ◽  
Louise Stinson ◽  
Betty Hatten
Keyword(s):  
Tissue Culture ◽  
Plastic Tissue Culture

In Vivo Interactions with (Tissue Culture Pretreated) Dermal Sheep Collagen

1991 ◽  
Vol 252 ◽  
Author(s):  
P. B. van Wachem ◽  
P. B. van Wachem ◽  
L. H. H. Olde Damink ◽  
P. J. Dijkstra ◽  
J. Feijen ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Death ◽  
Marked Reduction ◽  
Giant Cells ◽  
In Vitro Cytotoxicity ◽  
Cell Infiltration ◽  
Cytotoxic Effects ◽  
Lipid Formation

ABSTRACTPretreatment in tissue culture (TC) was previously found to markedly reduce the in vitro cytotoxicity of two types of crosslinked dermal sheep collagens (DSC's). This in vivo study confirms our in vitro results, in that TC-pretreatment of crosslinked DSC's resulted in the marked reduction or elimination of cytotoxic effects, such as increased cell infiltration, a deviant neutrophil-morphology, lipid formation and cell death. TC-pretreatment affected the crosslinked state of both DSC's in a different way, which could be deduced from the differences in gelatin-formation and presence of giant cells from macrophage- or fibroblast-origin. The results are explained in view of the differences in crosslinking.

Melatonin rescues dopamine neurons from cell death in tissue culture models of oxidative stress

1997 ◽  
Vol 768 (1-2) ◽  
pp. 317-326 ◽  
Author(s):  
Lorraine Iacovitti ◽  
Natalie D Stull ◽  
Kelly Johnston
Keyword(s):  
Oxidative Stress ◽  
Tissue Culture ◽  
Cell Death ◽  
Dopamine Neurons ◽  
Culture Models

scholarly journals COLD STORAGE OF IN VITRO RUBUS GERMPLASM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 695f-695
Author(s):  
Barbara M. Reed
Keyword(s):  
Tissue Culture ◽  
Cold Storage ◽  
Survival Rates ◽  
Storage Condition ◽  
Storage Conditions ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Glass Tubes ◽  
Plastic Tissue Culture

In vitro cold storage of Rubus germplasm was investigated using several environmental conditons and types of storage containers. Shoot cultures of Rubus species and cultivars were grown in either tissue culture bags or 20 × 150 mm glass tubes and compared for plant condition and survival under various storage conditions. Cultures stored at 10 C in the dark were in poor condition after 6 months. Cultures kept at 4 C were in much better condition and had higher survival rates after 18 months when stored with a 12 h daylength rather than total darkness. Overall there were no differences in survival or condition between cultures in tubes and bags. Contamination rates were 15% in tubes and 0% in bags. Plants in tissue culture bags could be stored for 9 months at 25 C with 16 h light when the nitrogen level of the MS medium was reduced to 25% and the medium volume was increased from 10 to 20 ml per bag. Genotype differences were apparent under all conditions tested. The best storage condition for Rubus germplasm was 4 C with 12 h light. Plastic tissue culture bags were preferred over tubes due to lower contamination rates.

scholarly journals 5-Azacitidine Induces Cell Death in a Tissue Culture of Brachypodium distachyon

2018 ◽  
Vol 19 (6) ◽  
pp. 1806 ◽  
Author(s):  
Alexander Betekhtin ◽  
Anna Milewska-Hendel ◽  
Lukasz Chajec ◽  
Magdalena Rojek ◽  
Katarzyna Nowak ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Death ◽  
Brachypodium Distachyon

scholarly journals Activation of Ca2+-Dependent Cation Current by Fluid Shear Force in Atrial Myocytes

2015 ◽  
Vol 108 (2) ◽  
pp. 112a
Author(s):  
Min-Jeong Son ◽  
Joon-Chul Kim ◽  
Ju Chen ◽  
Sun-Hee Woo
Keyword(s):  
Shear Force ◽  
Atrial Myocytes ◽  
Fluid Shear ◽  
Cation Current

Porous-bottom dishes for culture of polarized cells

1986 ◽  
Vol 251 (1) ◽  
pp. C136-C139 ◽  
Author(s):  
R. E. Steele ◽  
A. S. Preston ◽  
J. P. Johnson ◽  
J. S. Handler
Keyword(s):  
Tissue Culture ◽  
Cell Attachment ◽  
Collagen Membrane ◽  
Cellulose Ester ◽  
Polycarbonate Filter ◽  
Polarized Cells ◽  
Porous Surfaces ◽  
Plastic Tissue Culture

Porous-bottom dishes offer several advantages for growing and studying epithelia in culture. Many epithelia differentiate more on porous surfaces than on plastic tissue culture dishes. In addition, separate solutions can be maintained on each side of the epithelium and can be sampled easily for studies of transport and other polarized functions. We describe the fabrication of dishes with a cellulose ester filter, a collagen-coated polycarbonate filter, or a collagen membrane forming the surface for cell attachment at the bottom of the dish.

The effect of recombinant caspase 3 on porcine myofibrillar proteins

2007 ◽  
Vol 2007 ◽  
pp. 112-112
Author(s):  
CM. Kemp ◽  
T. Parr
Keyword(s):  
Skeletal Muscle ◽  
Cell Death ◽  
Preliminary Data ◽  
Shear Force ◽  
Caspase 3 ◽  
Proteolytic Enzymes ◽  
Myofibrillar Proteins ◽  
Caspase Activity ◽  
Conditioning Period

Meat tenderisation results from the weakening of the myofibrillar structures and has been attributed to endogenous proteolytic enzymes. It has been proposed that tenderization is a mulitenzymatic system and the process of slaughter and exsanguination would engage muscle cells in a form of cell death (Ouali et al., 2006). Caspases are primarily associated with apoptosis and once activated they target and cleave a number of substrates including components of the Z-disk and costameres. Recent studies have shown that caspases are active in skeletal muscle during the postmortem conditioning period and our preliminary data indicates that there is a relationship between caspase activity and shear force (Kemp et al., 2006). The aim of this study was to investigate whether recombinant caspase 3 was capable of porcine degrading myofibrillar proteins in vitro.

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