Sodium dodecyl sulphate gel electrophoretic preparation of protein standard human apolipoprotein B-48

2000 ◽  
Vol 742 (2) ◽  
pp. 421-426 ◽  
Author(s):  
B Saviana ◽  
L Pons ◽  
F Namour ◽  
D Quilliot ◽  
O Ziegler ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Apolipoprotein B ◽  
Sodium Dodecyl ◽  
Human Apolipoprotein

A Highly Sensitive Assay for Quantitation of Apolipoprotein B48 Using an Antibody to Human Apolipoprotein B and Enhanced Chemiluminescence

1997 ◽  
Vol 34 (2) ◽  
pp. 185-189 ◽  
Author(s):  
Darrin Smith ◽  
Spencer D Proctor ◽  
John C L Mamo
Keyword(s):  
Apolipoprotein B ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Apo B ◽  
Human Apolipoprotein ◽  
Enhanced Chemiluminescence ◽  
Coomassie Blue ◽  
Sds Page ◽  
One Step ◽  
Apolipoprotein B48

We describe a method for the rapid quantification of serum apolipoprotein B48 using a commercially available anti-apolipoprotein (apo)B antiserum and compare it to analytical sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with coomassie blue R250 staining. The method described here eliminates the need for de-lipidation of samples and only requires a one-step overnight ultracentrifugation. Western-blotting and enhanced chemiluminescence (ECL) visualization of proteins was approximately 10 times more sensitive than coomassie staining and generally took no longer to complete than staining/destaining of SDS-PAGE gels. The sensitivity of the antiserum/ECL technique enabled quantitation of fasting apolipoprotein B48 which could not be resolved by SDS-PAGE and Coomassie staining.

Identifikasi Daging Tikus Pada Produk Baso Dengan Metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

2018 ◽  
Vol 26 (2) ◽  
pp. 058
Author(s):  
Anna P. Roswiem ◽  
Triayu Septiani
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Sds Page

<em>Bahan<strong> </strong>baku untuk membuat baso adalah daging hewan, pada umumnya dari daging sapi, ayam, ikan dan babi. Di beberapa daerah di Indonesia terjadi kasus baso tikus. Tujuan penelitian ini adalah menguji ada tidaknya kandungan daging tikus pada produk baso yang dijual di pasar Cempaka Putih-Kecamatan Kramat Jakarta Pusat dan di pedagang baso atau mie baso di sekitar kampus Universitas YARSI Jakarta. Daging adalah protein salah satu metode untuk mengidentifikasi protein adalah metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).<strong> </strong>Hasil penelitian menunjukkan bahwa dari 6 sampel baso terindikasi ada 2 sampel baso dengan nomor 1 dan 5 yang dibuat dari campuran daging sapi dan tikus; ada 1 sampel baso dengan nomor 6 yang terbuat dari daging tikus; dan 2 sampel baso dengan nomor 2 dan 3 yang terbuat dari campuran sapi  dan babi, dan hanya 1 sampel baso dengan nomor sampel 4 yang benar-benar terbuat dari daging sapi.</em>

Ultrasound Effect on Molecules of Sodium Dodecyl Sulphate as Systems of Nanoparticles

2018 ◽  
Vol 10 (6) ◽  
pp. 06013-1-06013-5 ◽  
Author(s):  
I. G. Vorobiova ◽  
◽  
Yu. A. Mirgorod ◽  
A. S. Chekadanov ◽  
◽  
...  
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Ultrasound Effect

scholarly journals Structural Domains of Human Apolipoprotein B-100

1989 ◽  
Vol 264 (24) ◽  
pp. 14369-14375 ◽  
Author(s):  
G C Chen ◽  
S Zhu ◽  
D A Hardman ◽  
J W Schilling ◽  
K Lau ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Structural Domains ◽  
Human Apolipoprotein

scholarly journals Thrombin cleavage of apolipoprotein Bh of rabbit LDL: structural comparisons with human apolipoprotein B-100.

1992 ◽  
Vol 33 (6) ◽  
pp. 889-898
Author(s):  
A Leroy ◽  
G Castro ◽  
G Agnani ◽  
R Saïle ◽  
A Barkia ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Human Apolipoprotein ◽  
Thrombin Cleavage

The Kinetics of Methyl Methacrylate Miniemulsion Polymerization Characterized through a Fluorescence Method

2012 ◽  
Vol 178-181 ◽  
pp. 609-612
Author(s):  
Hai Ke Feng ◽  
Hua Yu Qiu ◽  
Li Yuan Ding ◽  
Cun Jin Xu
Keyword(s):  
Methyl Methacrylate ◽  
Anionic Surfactant ◽  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Miniemulsion Polymerization ◽  
Time Measurement ◽  
Fluorescence Method ◽  
Real Time Measurement ◽  
Measurement Results ◽  
Kinetics Of

In this paper, we followed the kinetics of methyl methacrylate (MMA) through a novel fluorescence method. The real-time measurement results show that in the regime of very low monomer contents, such as a solution containing 0.1 wt% of MMA with respect to water and with the anionic surfactant of sodium dodecyl sulphate (SDS), the kinetic of the miniemulsion could be followed by this embed fluorescence method. The processes of changing from emulsion to miniemulsion with different amount of surfactant and cosurfactant also have been monitored.

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