Enhancement of varicella-zoster virus detection in A-549 shell vials by use of freeze-thawed specimens, extended incubation, and “a centrifuged, not incubated” direct detection method

1998 ◽  
Vol 31 (4) ◽  
pp. 555-558 ◽  
Author(s):  
James P Brinker ◽  
Gary V Doern
Keyword(s):  
Varicella Zoster Virus ◽  
Detection Method ◽  
Direct Detection ◽  
Virus Detection ◽  
Varicella Zoster ◽  
Direct Detection Method

scholarly journals Single Molecule Investigation of Ag+ Interactions with Single Cytosine-, Methylcytosine- and Hydroxymethylcytosine-Cytosine Mismatches in a Nanopore

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Yong Wang ◽  
Bin-Quan Luan ◽  
Zhiyu Yang ◽  
Xinyue Zhang ◽  
Brandon Ritzo ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Binding Site ◽  
Single Molecule ◽  
Metal Ion ◽  
Detection Method ◽  
Direct Detection ◽  
Alpha Hemolysin ◽  
Duplex Stability ◽  
Dynamics Simulations ◽  
Direct Detection Method

Abstract Both cytosine-Ag-cytosine interactions and cytosine modifications in a DNA duplex have attracted great interest for research. Cytosine (C) modifications such as methylcytosine (mC) and hydroxymethylcytosine (hmC) are associated with tumorigenesis. However, a method for directly discriminating C, mC and hmC bases without labeling, modification and amplification is still missing. Additionally, the nature of coordination of Ag+ with cytosine-cytosine (C-C) mismatches is not clearly understood. Utilizing the alpha-hemolysin nanopore, we show that in the presence of Ag+, duplex stability is most increased for the cytosine-cytosine (C-C) pair, followed by the cytosine-methylcytosine (C-mC) pair and the cytosine-hydroxymethylcytosine (C-hmC) pair, which has no observable Ag+ induced stabilization. Molecular dynamics simulations reveal that the hydrogen-bond-mediated paring of a C-C mismatch results in a binding site for Ag+. Cytosine modifications (such as mC and hmC) disrupted the hydrogen bond, resulting in disruption of the Ag+ binding site. Our experimental method provides a novel platform to study the metal ion-DNA interactions and could also serve as a direct detection method for nucleobase modifications.

scholarly journals Anti-NMDAR encephalitis with concomitant varicella zoster virus detection and nonteratomatous malignancy

2019 ◽  
Vol 6 (2) ◽  
pp. e537
Author(s):  
Preeti A. Prakash ◽  
Jingxiao Jin ◽  
Kanwal Matharu ◽  
Tanu Garg ◽  
Wendy C. Tsai ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Virus Detection ◽  
Varicella Zoster ◽  
Nmdar Encephalitis

scholarly journals Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry.

1994 ◽  
Vol 42 (6) ◽  
pp. 815-822 ◽  
Author(s):  
D Egger ◽  
M Troxler ◽  
K Bienz
Keyword(s):  
In Situ Hybridization ◽  
Detection Method ◽  
Direct Detection ◽  
Ultrastructural Level ◽  
Electron Microscopic ◽  
Grain Sizes ◽  
Antibody Conjugates ◽  
Direct Detection Method

We performed light and electron microscopic in situ hybridization, according to the same protocol and without pretreatment of sections, on Lowicryl- and LR Gold-embedded cells. Digoxigenin (DIG)- or biotin-labeled riboprobes were visualized by direct or indirect immunodetection using commercially available gold-antibody conjugates with 0.8-10-nm gold grains. At the ultrastructural level, the main findings were that DIG-labeled probes gave a slightly higher labeling intensity (grains per signal) than biotin. The direct detection method produced a more compact signal, which led to better resolution at medium and high magnifications. Labeling intensities of all gold grain sizes were essentially equal. Grain sizes of 5 nm and larger were highly preferable because available enhancement methods are unsatisfactory for ultrasmall grains. The optimized immunodetection protocols are suitable for double hybridization with two different probes and for combined hybridization and immunocytochemistry.

Segmental zoster paresis as a cause for persistent fever in an immunocompromised patient

2021 ◽  
Vol 14 (10) ◽  
pp. e246015
Author(s):  
Renuka Murali Govind ◽  
Yun Cao
Keyword(s):  
Varicella Zoster Virus ◽  
Immunocompromised Patient ◽  
Virus Detection ◽  
Early Investigation ◽  
Molecular Testing ◽  
Limb Weakness ◽  
Varicella Zoster ◽  
Intravenous Acyclovir ◽  
High Index Of Suspicion ◽  
Delays In Diagnosis

Herpes zoster reactivation is a frequently encountered condition that can result in several uncommon complications. This case report highlights one such frequently overlooked complication, segmental zoster paresis. We discuss a case of prolonged fever and lower limb weakness in an immunocompromised patient with breast cancer on active chemotherapy after resolution of a herpetiform rash in the L2, L3 and L4 dermatomes. Early investigation with lumbar puncture, looking for cerebrospinal fluid pleocytosis, varicella zoster virus detection by PCR or molecular testing and immunoglobulins against varicella zoster virus, should be undertaken to support the diagnosis. Nerve conduction studies, electromyography and MRI of the spine can sometimes help with neurolocalisation. Intravenous acyclovir and a tapering course of steroids can help with resolution of symptoms. The variegate presentation can make diagnosis challenging. Awareness and a high index of suspicion can prevent delays in diagnosis and treatment and improve patient outcomes.

Molecular detection of human adenovirus in sediment using a direct detection method compared to the classical polyethylene glycol precipitation

2015 ◽  
Vol 213 ◽  
pp. 65-67 ◽  
Author(s):  
Rodrigo Staggemeier ◽  
Marina Bortoluzzi ◽  
Tatiana Moraes da Silva Heck ◽  
Tiago da Silva ◽  
Fernando Rosado Spilki ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Molecular Detection ◽  
Detection Method ◽  
Direct Detection ◽  
Human Adenovirus ◽  
Polyethylene Glycol Precipitation ◽  
Direct Detection Method
Sign in / Sign up

Export Citation Format

Share Document