1141 Neurite outgrowth from dissociated dorsal root ganglion cells on spinal gray and white matters of chick embryo: In vitro analysis using cryoculture system

1993 ◽  
Vol 18 ◽  
pp. S117
Author(s):  
Takashi Shiga ◽  
Toshio Shirai
Keyword(s):  
Chick Embryo ◽  
Dorsal Root Ganglion ◽  
Neurite Outgrowth ◽  
Dorsal Root ◽  
Ganglion Cells ◽  
Vitro Analysis ◽  
Dorsal Root Ganglion Cells ◽  
In Vitro Analysis

In vitro analysis of interactions between sensory neurons and skin: evidence for selective innervation of dermis and epidermis

Development ◽  
1985 ◽  
Vol 86 (1) ◽  
pp. 53-70
Author(s):  
J.-M. Verna
Keyword(s):  
Ganglion Cells ◽  
Neurite Growth ◽  
Epidermal Cells ◽  
Serum Free Medium ◽  
Diffusible Factor ◽  
Vitro Analysis ◽  
Dermal Cells ◽  
Dorsal Root Ganglion Cells ◽  
In Vitro Analysis

Axons from dorsal root ganglion cells cultured in a serum-free medium on poly-L-lysine or collagen substrates interact differently with dermis and epidermis. The orientation of neurite growth is not changed by encountering mesenchymal cells migrating from the outgrowth zone of a dermal explant, and neurites form close membrane associations with some dermal cells; in contrast, neurites strongly avoid epidermis and deviate around the edge of an epidermal explant. When cultures are grown on polylysine this avoidance behaviour occurs at a distance from the epidermis. It is suppressed in the presence of necrotic epidermal cells. We suggest that this avoidance is due to epidermal diffusible factor(s) which bind preferentially to polylysine. The possibility of an absence of specific recognition cues between neurites and epidermal cells is discussed.

Bullfrog dorsal root ganglion cells having tetrodotoxin-resistant spikes are endowed with nicotinic receptors

1989 ◽  
Vol 62 (3) ◽  
pp. 657-664 ◽  
Author(s):  
K. Morita ◽  
Y. Katayama
Keyword(s):  
Dorsal Root Ganglion ◽  
Conduction Velocity ◽  
Dorsal Root ◽  
Time Course ◽  
Ganglion Cells ◽  
Reversal Potential ◽  
Membrane Resistance ◽  
Fast Response ◽  
Dorsal Root Ganglion Cells

1. Intracellular recordings were made from bullfrog dorsal root ganglion (DRG) neurons in vitro. They were divided into three types, As, Ar, and C, according to their conduction velocity and their sensitivity to tetrodotoxin [TTX (less than or equal to 1 microM)]; an As neuron had a fast conduction velocity (13-50 m/s, mean = 31 m/s, n = 73) and TTX-sensitive sodium soma spikes: an Ar neuron showed a fast conduction velocity (4-28 m/s, mean = 14 m/s, n = 52) and TTX-resistant sodium soma spikes; and a C neuron had a slow conduction velocity (0.16-0.8 m/s, mean = 0.4 m/s, n = 49) and TTX-resistant sodium-calcium soma spikes. 2. Superfusion of acetylcholine [ACh (0.3 microM-1 mM)] produced a fast depolarization in 70% of Ar and in 50% of C neurons. No As neuron showed a fast depolarization in response to ACh. The ACh-induced fast response persisted in calcium-free or TTX-containing solutions. 3. The response in both Ar and C neurons was similar except in time course; the response was always more rapid in C than in Ar neurons. The response was always associated with a decreased membrane resistance and reversed in polarity at about -30 mV. The reversal potential varied with both sodium and potassium concentrations of the superfusing solutions. 4. Nicotine, (+)-tubocurarine [(+)-TC], and hexamethonium reversibly blocked the ACh fast response.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of different perfluorochemicals on dorsal root ganglion cells in vitro

1998 ◽  
Vol 236 (3) ◽  
pp. 182 ◽  
Author(s):  
D. Meller ◽  
A. J. Augustin ◽  
M. Spitznas ◽  
J. Lutz ◽  
K. Meller
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Ganglion Cells ◽  
Dorsal Root Ganglion Cells

Angiopoietin-1 promotes neurite outgrowth from dorsal root ganglion cells positive for Tie-2 receptor

2005 ◽  
Vol 320 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Joanna Kosacka ◽  
Maciej Figiel ◽  
J�rgen Engele ◽  
Heidegard Hilbig ◽  
Mariusz Majewski ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Neurite Outgrowth ◽  
Dorsal Root ◽  
Ganglion Cells ◽  
Dorsal Root Ganglion Cells ◽  
Angiopoietin 1
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