Enhanced synthesis of trisporic acid and β-carotene production in Blakeslea trispora by addition of a non-ionic surfactant, Span 20

1997 ◽  
Vol 84 (4) ◽  
pp. 330-332 ◽  
Author(s):  
Seon-Won Kim ◽  
Weon-Taek Seo ◽  
Young-Hoon Park
2013 ◽  
Vol 726-731 ◽  
pp. 315-319
Author(s):  
Wei Lian Hu ◽  
De Hui Dai

Non-ionic surfactant and oxygen-vectors may influence the growth of Blakeslea trispora and its primary product β-carotene. Compared with other non-ionic surfactants, Triton X-100 at 0.1% yielded the highest biomass production at 52.5 g/L and increased the production of β-carotene from 793.5 mg/L in the control to 923.5 mg/L. The β-carotene production and the yield of biomass were increased when oxygen-vectors were added to the medium. With 1% n-dodecane added on the 0 day, the biomass and β-carotene production was 17.2% and 10.0% higher than that of the control. The highest β-carotene and biomass production were obtained when 0.1% Triton X-100 and 1% n-dodecane were added together on the 0 day of fermentation.


HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 741-744 ◽  
Author(s):  
Gene E. Lester ◽  
John L. Jifon ◽  
D. J. Makus

Netted muskmelon [Cucumis melo L. (Reticulatus Group)] fruit quality (ascorbic acid, β-carotene, total free sugars, and soluble solids concentration (SSC)) is directly related to plant potassium (K) concentration during fruit growth and maturation. During reproductive development, soil K fertilization alone is often inadequate due to poor root uptake and competitive uptake inhibition from calcium and magnesium. Foliar applications of glycine-complexed K during muskmelon fruit development has been shown to improve fruit quality, however, the influence of organic-complexed K vs. an inorganic salt form has not been determined. This glasshouse study investigated the effects of two K sources: a glycine-complexed K (potassium metalosate, KM) and potassium chloride (KCl) (both containing 800 mg K/L) with or without a non-ionic surfactant (Silwet L-77) on melon quality. Orange-flesh muskmelon `Cruiser' was grown in a glasshouse and fertilized throughout the study with soil-applied N–P–K fertilizer. Starting at 3 to 5 d after fruit set, and up to 3 to 5 d before fruit maturity at full slip, entire plants were sprayed weekly, including the fruit, with KM or KCl with or without a surfactant. Fruit from plants receiving supplemental foliar K had significantly higher K concentrations in the edible middle mesocarp fruit tissue compared to control untreated fruit. Fruit from treated plants were also firmer, both externally and internally, than those from non-treated control plants. Increased fruit tissue firmness was accompanied by higher tissue pressure potentials of K treated plants vs. control. In general, K treated fruit had significantly higher SSC, total sugars, total ascorbic acid, and β-carotene than control fruit. Fall-grown fruit generally had higher SSC, total sugars, total ascorbic acid and β-carotene concentrations than spring-grown fruit regardless of K treatment. The effects of surfactant were not consistent but in general, addition of a surfactant tended to affect higher SSC and β-carotene concentrations.


2007 ◽  
Vol 42 (2) ◽  
pp. 289-293 ◽  
Author(s):  
Fang Xu ◽  
Qi-Peng Yuan ◽  
Yan Zhu

2004 ◽  
Vol 70 (9) ◽  
pp. 5589-5594 ◽  
Author(s):  
M. Rodríguez-Sáiz ◽  
B. Paz ◽  
J. L. de la Fuente ◽  
M. J. López-Nieto ◽  
W. Cabri ◽  
...  

ABSTRACT We cloned the carB and carRA genes involved in β-carotene biosynthesis from overproducing and wild-type strains of Blakeslea trispora. The carB gene has a length of 1,955 bp, including two introns of 141 and 68 bp, and encodes a protein of 66.4 kDa with phytoene dehydrogenase activity. The carRA gene contains 1,894 bp, with a single intron of 70 bp, and encodes a protein of 69.6 kDa with separate domains for lycopene cyclase and phytoene synthase. The estimated transcript sizes for carB and carRA were 1.8 and 1.9 kb, respectively. CarB from the β-carotene-overproducing strain B. trispora F-744 had an S528R mutation and a TAG instead of a TAA stop codon. The overproducing strain also had a P143S mutation in CarRA. Both B. trispora genes could complement mutations in orthologous genes in Mucor circinelloides and could be used to construct transformed strains of M. circinelloides that produced higher levels of β-carotene than did the nontransformed parent. The results show that these genes are conserved across the zygomycetes and that the B. trispora carB and carRA genes are functional and potentially useable to increase carotenoid production.


2021 ◽  
Vol 12 ◽  
Author(s):  
Liang Liu ◽  
Yu Ling Qu ◽  
Gui Ru Dong ◽  
Jing Wang ◽  
Ching Yuan Hu ◽  
...  

β-carotene is a precursor of vitamin A and has multiple physiological functions. Producing β-carotene by microbial fermentation has attracted much attention to consumers’ preference for natural products. This study focused on improving β-carotene production by constructing codon-adapted genes and minimizing intermediate accumulation. The codon-adapted CarRA and CarB genes from the industrial strain of Blakeslea trispora were integrated into the genome of the Yarrowia lipolytica to construct YL-C0, the baseline strain for producing β-carotene. Thereafter, the β-carotene biosynthetic pathway’s metabolic balance was accurately regulated to reduce the intermediates’ accumulation. Notably, the β-carotene content increased by 21 times to reach 12.5 dry cell weight (DCW) mg/g when minimizing HMG-CoA and FPP accumulation. Further, we improved the expression levels of the CarRA and CarB genes to minimize the accumulation of phytoene and lycopene. Total production of β-carotene of 1.7 g/L and 21.6 mg/g DCW was achieved. These results reveal that the rate-limiting enzymes CarRA and CarB of B. trispora exhibited higher catalytic activity than the same enzymes from other microorganisms. Promoting metabolic balance by minimizing the accumulation of intermediates is a very effective strategy for increasing β-carotene. The β-carotene-producing strain constructed in this study has established the foundation for its potential use in industrial production. These successful engineering strategies also provide a foundation for large-scale production of other terpenoids.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Umesh Luthra ◽  
Prabhakar Babu ◽  
Remya R.R. ◽  
Angeline Julius ◽  
Yogesh Patel ◽  
...  

Purpose β-Carotene is the most appropriate and significant precursor of vitamin A. Synthetic carotene supplements have been known to pose a threat to human health, making natural sources such as the indefensible choice for the production and extraction of carotene. Design/methodology/approach This study considers Blakeslea trispora, a filamentous fungus, as a source of production of carotenoids by fermentation and wet and dry mycelium were used to analyse and obtain better extraction results. Findings In this study, natural oils such as soy oil and cottonseed oil were incorporated into fermentation media to increase the production of carotene. For the optimization process, Plackett–Burman and one-factor-at-a-time (OVAT) models were identified as being of great value. Originality/value OVAT was carried out for corn starch because it plays a major role in the production of carotene and the corn starch at 30 g/L concentration has shown the maximum activity of 3.48 mg/gm. After optimizing process variables, submerged fermentation was eventually carried out under highly controlled media conditions. The resulting product was quantified using UV spectroscopy and extraction of carotene has been observed in the presence of various solvents. Among a range of solvents used, the methylene Di chloride produced-carotene at 86% recovery at a significantly lower temperature of 35°C.


2018 ◽  
Vol 47 (3) ◽  
pp. 189-195
Author(s):  
Serap Durakli Velioglu ◽  
Goksel Tirpanci Sivri

Purpose β-carotene, found naturally in many foods, has been widely used as a colourant in various products. The aim of this study is to optimize the production of β-carotene by Blakeslea trispora using bug-damaged wheat as the main nitrogen and carbon source. Design/methodology/approach Response surface methodology (RSM) has been used to determine the optimum concentrations of media components for the maximum β-carotene production. β-carotene concentration was determined quantitively using a spectrophotometric method. Findings A full quadratic model fit the data best for β-carotene production with a high R2 value (0.999). Using optimized media increased the production of β-carotene by B. trispora DSM-2387 and B. trispora DSM-2388 to a higher level (250.407 mg/l) than the control medium (72.195 mg/l). Originality/value This study showed that bug-damaged wheat, an agro-industrial waste having low economical value, may be used as a valuable substrate for microbial production of β-carotene, a natural food colourant possessing high economical value.


2017 ◽  
Vol 57 ◽  
pp. 57-63 ◽  
Author(s):  
Zijun He ◽  
Shizeng Wang ◽  
Yumeng Yang ◽  
Jing Hu ◽  
Can Wang ◽  
...  

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