Effect of Non-Ionic Surfactant and Oxygen-Vector on the Production of β-Carotene and Biomass from Blakeslea trispora

2013 ◽  
Vol 726-731 ◽  
pp. 315-319
Author(s):  
Wei Lian Hu ◽  
De Hui Dai

Non-ionic surfactant and oxygen-vectors may influence the growth of Blakeslea trispora and its primary product β-carotene. Compared with other non-ionic surfactants, Triton X-100 at 0.1% yielded the highest biomass production at 52.5 g/L and increased the production of β-carotene from 793.5 mg/L in the control to 923.5 mg/L. The β-carotene production and the yield of biomass were increased when oxygen-vectors were added to the medium. With 1% n-dodecane added on the 0 day, the biomass and β-carotene production was 17.2% and 10.0% higher than that of the control. The highest β-carotene and biomass production were obtained when 0.1% Triton X-100 and 1% n-dodecane were added together on the 0 day of fermentation.

2010 ◽  
Vol 64 (1) ◽  
Author(s):  
Sergey Kulichenko ◽  
Volodymyr Doroschuk ◽  
Natalia Gonta

AbstractThe influence of concentration conditions and acidity on the phase separation in non-ionic surfactants Triton X-100 solutions in the presence of phenol was investigated. It was shown that the addition of small amounts of phenol results in the decrease of the cloud point temperature of Triton X-100 solutions. On the other hand, the addition of phenol into the investigated system resulted in the decrease of the hydration values of surfactant-rich phases and the increase of their hydrophobicity. The extraction degree and distribution coefficient of phenol between the water and the surfactant-rich phases were studied. On the basis of data obtained the molar parts of water, phenol and Triton X-100 in the non-ionic surfactant-rich phases formed at different concentration conditions were calculated. Possibilities of the application of phenol-induced micellar extraction for microcomponents preconcentration were estimated.


HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 741-744 ◽  
Author(s):  
Gene E. Lester ◽  
John L. Jifon ◽  
D. J. Makus

Netted muskmelon [Cucumis melo L. (Reticulatus Group)] fruit quality (ascorbic acid, β-carotene, total free sugars, and soluble solids concentration (SSC)) is directly related to plant potassium (K) concentration during fruit growth and maturation. During reproductive development, soil K fertilization alone is often inadequate due to poor root uptake and competitive uptake inhibition from calcium and magnesium. Foliar applications of glycine-complexed K during muskmelon fruit development has been shown to improve fruit quality, however, the influence of organic-complexed K vs. an inorganic salt form has not been determined. This glasshouse study investigated the effects of two K sources: a glycine-complexed K (potassium metalosate, KM) and potassium chloride (KCl) (both containing 800 mg K/L) with or without a non-ionic surfactant (Silwet L-77) on melon quality. Orange-flesh muskmelon `Cruiser' was grown in a glasshouse and fertilized throughout the study with soil-applied N–P–K fertilizer. Starting at 3 to 5 d after fruit set, and up to 3 to 5 d before fruit maturity at full slip, entire plants were sprayed weekly, including the fruit, with KM or KCl with or without a surfactant. Fruit from plants receiving supplemental foliar K had significantly higher K concentrations in the edible middle mesocarp fruit tissue compared to control untreated fruit. Fruit from treated plants were also firmer, both externally and internally, than those from non-treated control plants. Increased fruit tissue firmness was accompanied by higher tissue pressure potentials of K treated plants vs. control. In general, K treated fruit had significantly higher SSC, total sugars, total ascorbic acid, and β-carotene than control fruit. Fall-grown fruit generally had higher SSC, total sugars, total ascorbic acid and β-carotene concentrations than spring-grown fruit regardless of K treatment. The effects of surfactant were not consistent but in general, addition of a surfactant tended to affect higher SSC and β-carotene concentrations.


Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1223 ◽  
Author(s):  
Evelin Farsang ◽  
Violetta Gaál ◽  
Ottó Horváth ◽  
Erzsébet Bárdos ◽  
Krisztián Horváth

It is well known that surfactants increase the solubility of hydrophobic organic compounds and cause adverse environmental effects. The removal of these compounds from the contaminated soil or ground-water is particularly difficult due to their water soluble feature. In this work, an ultra-high performance hydrophilic interaction liquid chromatographic method was developed for the separation of oligomers of Triton X-100 octylphenol-polyethoxylate non-ionic surfactant. Liquid chromatography-mass spectrometry (LC-MS) was used to identify the Triton X-100 compounds. There was a 44 mass unit difference between two adjacent peaks that is the molar mass of one ethylene oxide group (–CH 2 CH 2 O–). A quadratic retention model was applied for the estimation of retention of the examined non-ionic surfactant and the optimization of gradient elution conditions. The optimized method was suitable for the baseline separation of 28 Triton X-100 oligomers in five minutes.


2007 ◽  
Vol 42 (2) ◽  
pp. 289-293 ◽  
Author(s):  
Fang Xu ◽  
Qi-Peng Yuan ◽  
Yan Zhu

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