Chapter III Studies of the DNA binding activity of transcription factors in mapping brain function

2002 ◽  
pp. 45-58 ◽  
Author(s):  
Bozena Kaminska
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Brain Function ◽  
Binding Activity ◽  
Dna Binding Activity

scholarly journals Direct inhibition of the DNA-binding activity of POU transcription factors Pit-1 and Brn-3 by selective binding of a phenyl-furan-benzimidazole dication

2008 ◽  
Vol 36 (10) ◽  
pp. 3341-3353 ◽  
Author(s):  
Paul Peixoto ◽  
Yang Liu ◽  
Sabine Depauw ◽  
Marie-Paule Hildebrand ◽  
David W. Boykin ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Binding Activity ◽  
Direct Inhibition ◽  
Selective Binding ◽  
Dna Binding Activity

Ozone-induced IL-8 expression and transcription factor binding in respiratory epithelial cells

1997 ◽  
Vol 272 (3) ◽  
pp. L504-L511 ◽  
Author(s):  
I. Jaspers ◽  
E. Flescher ◽  
L. C. Chen
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Epithelial Cells ◽  
Inflammatory Cells ◽  
Binding Activity ◽  
Ozone Exposure ◽  
Air Exposure ◽  
Dna Binding Activity ◽  
Nf Kappab

Ozone, one of the most reactive oxidant gases to which humans are routinely exposed, induces inflammation in the lower airways. The airway epithelium is one of the first targets that inhaled ozone will encounter, but its role in airway inflammation is not well understood. Expression of inducible genes involved in the inflammatory response, such as interleukin (IL)-8, is controlled by transcription factors. Expression of the IL-8 gene is regulated by the transcription factors nuclear factor (NF)-kappaB, NF-IL-6, and possibly activator protein-1 (AP-1). Type II-like epithelial cells (A549) were grown on a collagen-coated membrane and exposed in vitro to 0.1 ppm ozone or air. Exposure to ozone induced DNA-binding activity of NF-kappaB, NF-IL-6, and AP-1. IL-8 mRNA and IL-8 protein levels were also increased after ozone exposure. These results link ozone-induced DNA-binding activity of transcription factors and the production of IL-8 by epithelial cells thus demonstrating a potential cellular cascade resulting in the recruitment of inflammatory cells into the airway lumen.

scholarly journals CD43-mediated Signals Induce DNA Binding Activity of AP-1, NF-AT, and NFκB Transcription Factors in Human T Lymphocytes

2000 ◽  
Vol 275 (40) ◽  
pp. 31460-31468 ◽  
Author(s):  
M. Angélica Santana ◽  
Gustavo Pedraza-Alva ◽  
Norma Olivares-Zavaleta ◽  
Vicente Madrid-Marina ◽  
Vaclav Horejsi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
T Lymphocytes ◽  
Dna Binding ◽  
Binding Activity ◽  
Human T Lymphocytes ◽  
Dna Binding Activity

scholarly journals Expression of the genes encoding CCAAT-enhancer binding protein isoforms in the mouse mammary gland during lactation and involution

1998 ◽  
Vol 334 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Georgios SABATAKOS ◽  
Gareth E. DAVIES ◽  
Maria GROSSE ◽  
Anthony CRYER ◽  
Dipak P. RAMJI
Keyword(s):  
Transcription Factors ◽  
Mammary Gland ◽  
Dna Binding ◽  
Binding Protein ◽  
Mouse Mammary Gland ◽  
Binding Activity ◽  
Protein Isoforms ◽  
Dna Binding Activity ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the activation of gene expression in the mammary gland during lactation. We have therefore investigated the detailed expression profile of the C/EBP family during lactation and involution of the mouse mammary gland. The expression of C/EBPβ and C/EBPδ mRNA was low during lactation, increased dramatically at the beginning of involution and remained constant thereafter. In contrast, C/EBPα mRNA expression was relatively high during the early stages of lactation, declined to low levels during the late stages of lactation and at the start of involution, and increased again during involution. Electrophoretic mobility-shift assays showed a close correlation between the expression of the C/EBP genes and the functional C/EBP DNA-binding activity and, additionally, demonstrated the participation of heterodimers, formed from among the three proteins, in DNA–protein interactions. The DNA-binding activity of the activator protein 1 (AP1) family of transcription factors was also induced during involution. These results therefore point to potentially important regulatory roles for both the C/EBP and the AP1 family during lactation and involution of the mammary gland.

Examination of DNA-binding activity of neuronal transcription factors by electrophoretical mobility shift assay

1998 ◽  
Vol 2 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Koichiro Kako ◽  
Hisanori Wakamatsu ◽  
Toshiyuki Hamada ◽  
Marek Banasik ◽  
Keiko Ohata ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Binding Activity ◽  
Mobility Shift ◽  
Dna Binding Activity ◽  
Mobility Shift Assay

scholarly journals The DNA Binding Activity of C/EBP Transcription Factors Is Regulated in the G Phase of the Hepatocyte Cell Cycle

1995 ◽  
Vol 270 (30) ◽  
pp. 18123-18132 ◽  
Author(s):  
Basabi Rana ◽  
Yuhong Xie ◽  
David Mischoulon ◽  
Nancy L. R. Bucher ◽  
Stephen R. Farmer
Keyword(s):  
Cell Cycle ◽  
Transcription Factors ◽  
Dna Binding ◽  
Binding Activity ◽  
Dna Binding Activity

scholarly journals Development of a High-Throughput Plate-Based Chemiluminescent Transcription Factor Assay

2004 ◽  
Vol 9 (4) ◽  
pp. 334-342 ◽  
Author(s):  
Christopher Rosenau ◽  
Daryl Emery ◽  
Barbara Kaboord ◽  
M. Walid Qoronfleh
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
High Throughput ◽  
Transcription Initiation ◽  
Dynamic Range ◽  
Colorimetric Detection ◽  
Binding Activity ◽  
Dna Binding Activity ◽  
Cell Growth And Differentiation ◽  
Factor Assay

Transcription factors are DNA-binding proteins that regulate the expression of specific genes by controlling transcription initiation. Two families of transcription factors, NFκB and AP-1, play pivotal roles in controlling important cellular processes ranging from normal cell growth and differentiation to apoptosis and cancer. Identifying changes in the DNA-binding activity of these factors is essential to understanding the regulation of these processes. We have developed a high-throughput DNA-based ELISA capable of monitoring activated levels of NFκB (p50 and p65) and AP-1 (c-Jun and c-Fos). This chemiluminescent assay utilizes a 96-well plate format, eliminating the throughput challenges imposed by traditional gel shift assays and exceeding the sensitivity and dynamic range of standard colorimetric detection systems. The sensitivity of this assay enables distinction between subtle as well as dramatic differences in the DNA-binding activity of these factors that result from the treatment of cells with various inhibitors or activating agents.

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