Effects of epidermal growth factor on collagen expression by rat kidney mesangial cells in culture

2000 ◽  
Vol 19 (1) ◽  
pp. 47-59 ◽  
Author(s):  
Michael A. Haralson ◽  
Samuel J. DiMari ◽  
Richard L. Hoover ◽  
Raymond C. Harris
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Mesangial Cells ◽  
Rat Kidney ◽  
Cells In Culture ◽  
Collagen Expression ◽  
Epidermal Growth

A role for G-proteins in the epidermal growth factor stimulation of phospholipase A2 in rat kidney mesangial cells

1990 ◽  
Vol 10 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Nashrudeen Hack ◽  
Paula Clayman ◽  
Karl Skorecki
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Phospholipase A2 ◽  
G Protein ◽  
Mesangial Cells ◽  
Rat Kidney ◽  
Glomerular Mesangial Cells ◽  
Epidermal Growth ◽  
Stimulation Of

We have previously demonstrated phospholipase C (PLC) independent activation of phospholipase A2(PLA2) by epidermal growth factor (EGF) in glomerular mesangial cells in culture. In the current study using glass beads to permeabilize [3H]- or [14C]-arachidonate labelled mesangial cells we demonstrate that guanine nucleotides modulate the EGF-mediated stimulation of arachidonic acid release (75% inhibition with 100 μM GDPβS and 108% augmentation with 100 μM GTPγS). GTPγS alone stimulated both the release of free arachidonic acid and production of diacylglycerol (DAG), while EGF itself neither stimulated DAG nor augmented the DAG response to GTPγS. These findings suggest the intermediacy of a G-protein in PLC-independent stimulation of PLA2 by a growth factor, and provide a model system for determining the relationship between G-protein intermediacy and the intrinsic tyrosine kinase activity of the growth factor receptor.

Perinatal Development of the Rat Kidney: Proliferative Activity and Epidermal Growth Factor

Neonatology ◽  
2001 ◽  
Vol 79 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Toshiya Okada ◽  
Asako Iwamoto ◽  
Ken Kusakabe ◽  
Masafumi Mukamoto ◽  
Yasuo Kiso ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Proliferative Activity ◽  
Rat Kidney ◽  
Perinatal Development ◽  
Epidermal Growth

Interaction of epidermal growth factor and transforming growth factor beta in human prostatic epithelial cells in culture

The Prostate ◽  
1992 ◽  
Vol 21 (2) ◽  
pp. 133-143 ◽  
Author(s):  
Debra M. Sutkowski ◽  
Chau-Jye Fong ◽  
Julia A. Sensibar ◽  
Alfred W. Rademaker ◽  
Edward R. Sherwood ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Cells In Culture ◽  
Epidermal Growth ◽  
Growth Factor Beta
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