Free cholesterol fiber-optic biosensor for serum samples with simplex optimization

1997 ◽  
Vol 12 (3) ◽  
pp. 233-240 ◽  
Author(s):  
M.D. Marazuela ◽  
B. Cuesta ◽  
M.C. Moreno-Bondi ◽  
A. Quejido
Keyword(s):  
Free Cholesterol ◽  
Fiber Optic ◽  
Serum Samples ◽  
Simplex Optimization

scholarly journals Errata: Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

2011 ◽  
Vol 16 (1) ◽  
pp. 019801 ◽  
Author(s):  
Chun Wei Wang ◽  
Upender Manne ◽  
Vishnu B. Reddy ◽  
Denise K. Oelschlager ◽  
Venkat R. Katkoori ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Fiber Optic ◽  
Quantitative Estimation ◽  
Serum Samples ◽  
Tapered Fiber

scholarly journals Quantifying Serum Antiplague Antibody with a Fiber-Optic Biosensor

1998 ◽  
Vol 5 (5) ◽  
pp. 609-612 ◽  
Author(s):  
George P. Anderson ◽  
Keeley D. King ◽  
Lynn K. Cao ◽  
Meagan Jacoby ◽  
Frances S. Ligler ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Cells ◽  
Serum Samples ◽  
Blood Samples ◽  
Competitive Assay ◽  
Protein Toxins ◽  
Whole Blood Samples ◽  
Good Agreement

ABSTRACT The fiber-optic biosensor, originally developed to detect hazardous biological agents such as protein toxins or bacterial cells, has been utilized to quantify the concentration of serum antiplague antibodies. This biosensor has been used to detect and quantify the plague fraction 1 antigen in serum, plasma, and whole-blood samples, but its ability to quantify serum antibodies has not been demonstrated. By using a competitive assay, the concentration of serum antiplague antibodies was ascertained in the range of 2 to 15 μg/ml. By making simple dilutions, concentrations for 11 serum samples whose antiplague antibody concentrations were unknown were determined and were found to be in good agreement with enzyme-linked immunosorbent assay results. The competitive assay method could be used to effectively determine the exposure to plague of animals or humans or could be applied to other diseases, such as hepatitis or AIDS, where the presence of antibodies is used to diagnose infection.

Evanescent Wave Fiber Optic Immunosensor for the Determination of Lactate Dehydrogenase in Serum Samples

1994 ◽  
pp. 254
Author(s):  
Teresa McCormack ◽  
Richard O'Kennedy ◽  
Gerard O'Keeffe ◽  
Brian MacCraith
Keyword(s):  
Lactate Dehydrogenase ◽  
Evanescent Wave ◽  
Fiber Optic ◽  
Serum Samples

Microaffinity chromatographic separation and characterization of lipoprotein fractions in rat and mongolian gerbil serum

1993 ◽  
Vol 39 (9) ◽  
pp. 1861-1867 ◽  
Author(s):  
J C Tschantz ◽  
G I Sunahara
Keyword(s):  
Free Cholesterol ◽  
Meriones Unguiculatus ◽  
Lipoprotein Fraction ◽  
Mongolian Gerbils ◽  
Serum Samples ◽  
Commercial Diet ◽  
Chromatographic Procedure ◽  
The Mean ◽  
Cholesterol Supplementation

Abstract A rapid, routine microaffinity chromatographic procedure with spectrometric detection was evaluated to measure the concentration of total and free cholesterol, triglycerides, and phospholipids linked to the alpha- and beta-/pre-beta-lipoproteins in very small serum samples from small experimental animals and from humans. This procedure overcomes many of the limitations presented by methods involving ultracentrifugation, electrophoresis, or precipitation. Using this method, we report that in 35 adult male Mongolian gerbils (Meriones unguiculatus) fed a basal commercial diet with no cholesterol supplementation, the mean (+/- SD) concentration of total and free cholesterol linked to the alpha-lipoprotein fraction (in mmol/L) is 2.85 +/- 0.81 and 0.33 +/- 0.14, respectively; phospholipids were 1.99 +/- 0.41, and triglycerides were 0.64 +/- 0.42. The concentration (mmol/L) of total and free cholesterol associated with the beta-/pre-beta-lipoprotein fraction is 0.92 +/- 0.26 and 0.22 +/- 0.06, respectively; phospholipids were 0.28 +/- 0.17, and triglycerides were 0.49 +/- 0.31.

scholarly journals Using serum metabolomics analysis to predict sub-clinical atherosclerosis in patients with SLE

2020 ◽  
Author(s):  
Leda Coelewij ◽  
Kirsty E Waddington ◽  
George A Robinson ◽  
Elvira Chocano ◽  
Thomas McDonnell ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Random Forest ◽  
Lupus Erythematosus ◽  
Free Cholesterol ◽  
Support Vector ◽  
Resonance Spectroscopy ◽  
Serum Samples ◽  
Cvd Risk ◽  
Increased Risk ◽  
Atherosclerosis Development

Background: Patients with systemic lupus erythematosus (SLE) have an increased risk of developing cardiovascular disease (CVD) and 30-40% have sub-clinical atherosclerosis on vascular ultrasound scanning. Standard measurements of serum lipids in clinical practice do not predict CVD risk in patients with SLE. We hypothesise that more detailed analysis of lipoprotein taxonomy could identify better predictors of CVD risk in SLE. Methods: Eighty patients with SLE and no history of CVD underwent carotid and femoral ultrasound scans; 30 had atherosclerosis plaques (SLE-P) and 50 had no plaques (SLE-NP). Serum samples obtained at the time of the scan were analysed using a lipoprotein-focused metabolomics platform assessing 228 metabolites by nuclear magnetic resonance spectroscopy. Data was analysed using logistic regression and five binary classification models with 10-fold cross validation; decision tree, random forest, support vector machine and lasso (Least Absolute Shrinkage and Selection Operator) logistic regression with and without interactions. Results: Univariate logistic regression identified four metabolites associated with the presence of sub-clinical plaque; three subclasses of very low density lipoprotein (VLDL) (percentage of free cholesterol in medium and large VLDL particles and percentage of phospholipids in chylomicrons and extremely large VLDL particles) and Leucine. Together with age, these metabolites were also within the top features identified by the lasso logistic regression (with and without interactions) and random forest machine learning models. Logistic regression with interactions differentiated between SLE-P and SLE-NP with greatest accuracy (0.800). Notably, percentage of free cholesterol in large VLDL particles and age were identified by all models as being important to differentiate between SLE-P and SLE-NP patients. Conclusion: Serum metabolites are a promising biomarker for prediction of sub-clinical atherosclerosis development in SLE patients and could provide novel insight into mechanisms of early atherosclerosis development.

scholarly journals Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples

2010 ◽  
Vol 15 (6) ◽  
pp. 067005 ◽  
Author(s):  
Chun Wei Wang ◽  
Upender Manne ◽  
Vishnu B. Reddy ◽  
Denise K. Oelschlager ◽  
Venkat R. Katkoori ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Fiber Optic ◽  
Quantitative Estimation ◽  
Serum Samples ◽  
Tapered Fiber

Enzyme-based fiber-optic device for the determination of total and free cholesterol

1992 ◽  
Vol 256 (2) ◽  
pp. 263-268 ◽  
Author(s):  
A. Krug ◽  
A.A. Suleiman ◽  
G.G. Guilbault
Keyword(s):  
Free Cholesterol ◽  
Fiber Optic

A Fiber Optic Enzyme Sensor for the Determination of Urea in Serum Samples

1994 ◽  
pp. 233
Author(s):  
Florian Schubert ◽  
Cord Müller ◽  
Thomas Scheper
Keyword(s):  
Fiber Optic ◽  
Serum Samples ◽  
Enzyme Sensor

Imaging and diffraction modes in Scanning Transmission Electron Microscopy

1986 ◽  
Vol 44 ◽  
pp. 684-687
Author(s):  
J. M. Cowley ◽  
R. Glaisher ◽  
J. A. Lin ◽  
H.-J. Ou
Keyword(s):  
Scanning Transmission Electron Microscopy ◽  
High Efficiency ◽  
Fiber Optic ◽  
Image Intensifier ◽  
Detector System ◽  
Detector Plane ◽  
Secondary Electrons ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Special Detector

Some of the most important applications of STEM depend on the variety of imaging and diffraction made possible by the versatility of the detector system and the serial nature, of the image acquisition. A special detector system, previously described, has been added to our STEM instrument to allow us to take full advantage of this versatility. In this, the diffraction pattern in the detector plane may be formed on either of two phosphor screens, one with P47 (very fast) phosphor and the other with P20 (high efficiency) phosphor. The light from the phosphor is conveyed through a fiber-optic rod to an image intensifier and TV system and may be photographed, recorded on videotape, or stored digitally on a frame store. The P47 screen has a hole through it to allow electrons to enter a Gatan EELS spectrometer. Recently a modified SEM detector has been added so that high resolution (10Å) imaging with secondary electrons may be used in conjunction with other modes.

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