Antitumor Effects of a Monoclonal Antibody That Binds Anionic Phospholipids on the Surface of Tumor Blood Vessels in Mice

2006 ◽  
Vol 2006 ◽  
pp. 378-379
Author(s):  
M.S. Gordon
Keyword(s):  
Monoclonal Antibody ◽  
Blood Vessels ◽  
Antitumor Effects ◽  
Anionic Phospholipids ◽  
Tumor Blood Vessels

Antitumor Effects of a Monoclonal Antibody that Binds Anionic Phospholipids on the Surface of Tumor Blood Vessels in Mice

2005 ◽  
Vol 11 (4) ◽  
pp. 1551-1562 ◽  
Author(s):  
Sophia Ran ◽  
Jin He ◽  
Xianming Huang ◽  
Melina Soares ◽  
Douglas Scothorn ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Blood Vessels ◽  
Antitumor Effects ◽  
Anionic Phospholipids ◽  
Tumor Blood Vessels

Fibrin Deposition in Squamous Cell Carcinomas of the Larynx and Hypopharynx

1998 ◽  
Vol 80 (11) ◽  
pp. 767-772 ◽  
Author(s):  
Helga Bárdos ◽  
Attila Juhász ◽  
Gábor Répássy ◽  
Róza Ádány
Keyword(s):  
Monoclonal Antibody ◽  
Connective Tissue ◽  
Tumor Cell ◽  
Blood Vessels ◽  
Squamous Cell ◽  
Close Association ◽  
Squamous Cell Carcinomas ◽  
Coagulation Cascade ◽  
Fibrin Deposition ◽  
Tumor Blood Vessels

SummaryExtravascular fibrin deposition is frequently observed within and around neoplastic tissue and has been implicated in various aspects of tumor growth. The distribution of fibrin deposits was investigated in squamous cell carcinomas representing different stages of tumor progression of the larynx (n = 25) and hypopharynx (n = 9) by immunofluorescent techniques. Double and treble labelings were used to detect fibrinogen and fibrin in combination with marker antigens for tumor cells (cytokeratin), endothelial cells (von Willebrand factor), macrophages (recognized by KiM7), as well as factor XIII subunit A (FXIIIA) and tenascin (an embryonic extracellular matrix protein newly expressed during tumorigenesis). All tissue samples showed specific staining for fibrinogen/fibrin. Fibrin deposition was localized almost exclusively in the connective tissue compartment of tumors with characteristic accumulation at the interface of connective tissue and the tumorous parenchyma. In certain tumor samples showing highly invasive characteristics, fibrin deposits were observed in close association with tumor blood vessels in the tumor cell nodules. The overlapping reactions with polyclonal antibody to fibrinogen/fibrin and monoclonal antibody to fibrin indicate the activation of the coagulation cascade resulting in in situ thrombin activation and fibrin formation. Fibrin was crosslinked and stabilized by FXIIIA as revealed by urea insolubility test. Accumulation of phagocytozing macrophages detected by Ki M7 monoclonal antibody could be seen in areas of fibrin deposition. The blood coagulation factor XIIIA was detected in and around the cells labeled with Ki M7 antibody. Tenascin and fibrin deposits were found in the same localization in the tumor stroma and in association with tumor blood vessels within the tumor cell nodules. Neither fibrin nor tenascin were detected in the histologically normal tissue adjacent to tumors. The close association between fibrin deposits and macrophage accumulation strongly suggests the active participation of tumor-associated macrophages in the formation of stabilized intratumoral fibrin that facilitates tumor matrix generation and tumor angiogenesis.

scholarly journals Three-dimensional relationships between tumor cells and microcirculation with double cyanine immunolabeling, laser scanning confocal microscopy, and computer-assisted reconstruction: an alternative to cast corrosion preparations.

1994 ◽  
Vol 42 (5) ◽  
pp. 681-686 ◽  
Author(s):  
V Rummelt ◽  
L M Gardner ◽  
R Folberg ◽  
S Beck ◽  
B Knosp ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Tumor Cells ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Tumor Blood Vessels ◽  
The Relationship ◽  
Scanning Electron

The morphology of the microcirculation of uveal melanomas is a reliable market of tumor progression. Scanning electron microscopy of cast corrosion preparations can generate three-dimensional views of these vascular patterns, but this technique sacrifices the tumor parenchyma. Formalin-fixed wet tissue sections 100-150 microns thick from uveal melanomas were stained with the lectin Ulex europaeus agglutinin I (UEAI) and proliferating cell nuclear antigen (PCNA) to demonstrate simultaneously the tumor blood vessels and proliferating tumor cells. Indocarbocyanine (Cy3) was used as a fluorophore for UEAI and indodicarbocyanine (Cy5) was used for PCNA. Double labeled sections were examined with a laser scanning confocal microscope. Images of both stains were digitized at the same 5-microns intervals and each of the two images per interval was combined digitally to form one image. These combined images were visualized through voxel processing to study the relationship between melanoma cells expressing PCNA and various microcirculatory patterns. This technique produces images comparable to scanning electron microscopy of cast corrosion preparations while permitting simultaneous localization of melanoma cells expressing PCNA. The microcirculatory tree can be viewed from any perspective and the relationship between tumor cells and the tumor blood vessels can be studied concurrently in three dimensions. This technique is an alternative to cast corrosion preparations.

scholarly journals Increasing Damage to Tumor Blood Vessels during Motexafin Lutetium-PDT through Use of Low Fluence Rate

2010 ◽  
Vol 174 (3) ◽  
pp. 331-340 ◽  
Author(s):  
Theresa M. Busch ◽  
Hsing-Wen Wang ◽  
E. Paul Wileyto ◽  
Guoqiang Yu ◽  
Ralph M. Bunte
Keyword(s):  
Blood Vessels ◽  
Fluence Rate ◽  
Tumor Blood Vessels
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