Temporal regulation of Drosophila salivary gland degeneration by the Broad-Complex transcription factors

2002 ◽  
Vol 140 (1-3) ◽  
pp. 67-78 ◽  
Author(s):  
Silvia Kuchárová-Mahmood ◽  
Ivan Raška ◽  
Bernard M. Mechler ◽  
Robert Farkaš
Keyword(s):  
Transcription Factors ◽  
Salivary Gland ◽  
Temporal Regulation ◽  
Broad Complex

scholarly journals BRN2 is a non-canonical melanoma tumor-suppressor

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Michael Hamm ◽  
Pierre Sohier ◽  
Valérie Petit ◽  
Jérémy H. Raymond ◽  
Véronique Delmas ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Tumor Suppressor ◽  
The Other ◽  
Pi3k Signaling ◽  
Melanoma Tumor ◽  
Temporal Regulation ◽  
Metastatic Colonization ◽  
Pou Domain

AbstractWhile the major drivers of melanoma initiation, including activation of NRAS/BRAF and loss of PTEN or CDKN2A, have been identified, the role of key transcription factors that impose altered transcriptional states in response to deregulated signaling is not well understood. The POU domain transcription factor BRN2 is a key regulator of melanoma invasion, yet its role in melanoma initiation remains unknown. Here, in a BrafV600EPtenF/+ context, we show that BRN2 haplo-insufficiency promotes melanoma initiation and metastasis. However, metastatic colonization is less efficient in the absence of Brn2. Mechanistically, BRN2 directly induces PTEN expression and in consequence represses PI3K signaling. Moreover, MITF, a BRN2 target, represses PTEN transcription. Collectively, our results suggest that on a PTEN heterozygous background somatic deletion of one BRN2 allele and temporal regulation of the other allele elicits melanoma initiation and progression.

scholarly journals Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis

Development ◽  
2010 ◽  
Vol 137 (15) ◽  
pp. e1508-e1508
Author(s):  
J. E. Kugler ◽  
S. Gazdoiu ◽  
I. Oda-Ishii ◽  
Y. J. Passamaneck ◽  
A. J. Erives ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Ciona Intestinalis ◽  
Temporal Regulation ◽  
Muscle Gene

scholarly journals Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and Broad-Complex gene activity

2003 ◽  
Vol 259 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Lorenzo F Sempere ◽  
Nicholas S Sokol ◽  
Edward B Dubrovsky ◽  
Edward M Berger ◽  
Victor Ambros
Keyword(s):  
Drosophila Melanogaster ◽  
Microrna Expression ◽  
Gene Activity ◽  
Temporal Regulation ◽  
Broad Complex

scholarly journals Three neighboring genes interact with the Broad-Complex and the Stubble-stubbloid locus to affect imaginal disc morphogenesis in Drosophila.

Genetics ◽  
1991 ◽  
Vol 127 (4) ◽  
pp. 747-759 ◽  
Author(s):  
P J Gotwals ◽  
J W Fristrom
Keyword(s):  
Drosophila Melanogaster ◽  
Transcription Factors ◽  
Genetic Markers ◽  
Imaginal Disc ◽  
Wing Disc ◽  
Unit Interval ◽  
Genetic Interactions ◽  
Wild Type ◽  
Recessive Lethal ◽  
Broad Complex

Abstract The Broad-Complex (BR-C) is a complex regulatory locus at 2B-5 on the X chromosome of Drosophila melanogaster. The wild-type BR-C products are apparent transcription factors necessary for imaginal disc morphogenesis. Alleles of the Stubble-stubbloid (Sb-sbd) locus at 89B9-10 act as dominant enhancers of broad alleles of the BR-C. Sb-sbd wild-type products are necessary for appendage elongation. We report, here, on three new loci implicated in imaginal disc morphogenesis based on their genetic interactions with both BR-C and/or Sb-sbd mutants. Enhancer of broad (E(br)) was identified as a dominant enhancer of the br1 allele of the BR-C and is a recessive lethal. Mapping of E(br) has led to the identification of two loci, blistered and l(2)B485, mutants of which interact with E(br) and the Sb-sbd locus. Blistered, but not l(2)B485, interacts strongly with the BR-C. Alleles of the blistered locus are viable and disrupt proper wing disc morphogenesis independent of genetic interactions. All three loci map within the 0.6-map unit interval between the genetic markers speck and Irregular facets and to the cytological region 60C5-6; 60E9-10 at the tip of chromosome 2R. Genetic evidence is consistent with the view that the BR-C regulates blistered.

scholarly journals A BTB-Domain Transcription factor Recruits Chromatin Remodelers and a Histone Chaperone during the exit from Pluripotency

2020 ◽  
Author(s):  
Daniel Olivieri ◽  
Sujani Paramanathan ◽  
Anaïs F. Bardet ◽  
Daniel Hess ◽  
Sébastien A. Smallwood ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Transcription Factor ◽  
Transcription Factors ◽  
State Transition ◽  
Histone Chaperone ◽  
Genetic Screen ◽  
Chromatin Remodelers ◽  
Btb Domain ◽  
Transcriptional Modulators ◽  
Broad Complex

ABSTRACTTranscription factors (TFs) harboring a btb (Broad-Complex, Tramtrack and Bric a brac) domain play important roles in development and disease. They are thought to recruit transcriptional modulators to DNA through their btb domain. However, a systematic molecular understanding of this TF family is lacking. Here, we identify the zinc finger btb-TF Zbtb2 in a genetic screen for regulators of exit from pluripotency and dissect its mechanistic mode of action. We show that ZBTB2 binds the chromatin remodeler Ep400 to mediate downstream transcription. Independently, the btb domain directly interacts with the chromatin remodeller NuRD and the histone chaperone HiRA via the GATAD2A/B and UBN2 subunits, respectively. NuRD recruitment is a common feature of btb-TFs and we propose by phylogenetic analysis that this is an evolutionary ancient property. Binding to UBN2, in contrast, is specific to ZBTB2 and requires a C-terminal extension of the btb domain. This study therefore identifies a btb-domain TF that recruits chromatin modifiers and a histone chaperone during a developmental cell state transition, and defines unique and shared molecular functions of the btb-domain TF family.

Downregulation of the tissue-specific transcription factor Fork head by Broad-Complex mediates a stage-specific hormone response

Development ◽  
2001 ◽  
Vol 128 (19) ◽  
pp. 3729-3737 ◽  
Author(s):  
Nathalie Renault ◽  
Kirst King-Jones ◽  
Michael Lehmann
Keyword(s):  
Salivary Gland ◽  
Adult Development ◽  
Target Genes ◽  
Regulatory Gene ◽  
Specific Gene ◽  
Tissue Specific ◽  
Fork Head ◽  
Salivary Gland Secretion ◽  
Pleiotropic Functions ◽  
Broad Complex

Drosophila development is coordinated by pulses of the steroid hormone 20-hydroxyecdysone (20E). During metamorphosis, the 20E-inducible Broad-Complex (BR-C) gene plays a key role in the genetic hierarchies that transduce the hormone signal, being required for the destruction of larval tissues and numerous aspects of adult development. Most of the known BR-C target genes, including the salivary gland secretion protein (Sgs) genes, are terminal differentiation genes that are thought to be directly regulated by BR-C-encoded transcription factors. Here, we show that repression of Sgs expression is indirectly controlled by the BR-C through transcriptional down-regulation of fork head, a tissue-specific gene that plays a central role in salivary gland development and is required for Sgs expression. Our results demonstrate that integration of a tissue-specific regulatory gene into a 20E-controlled genetic hierarchy provides a mechanism for hormonal repression. Furthermore, they suggest that the BR-C is placed at a different position within the 20E-controlled hierarchies than previously assumed, and that at least part of its pleiotropic functions are mediated by tissue-specific regulators.

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