Estrogen receptor α and progesterone receptor A and B concentration and localization in the lower uterine segment in term parturition

SummaryIt is well established that estrogen and progesterone are critical endogenous hormones that are essential for implantation and pregnancy in females. However, the distribution of estrogen receptor α (ERα) and progesterone receptor (PR) in female reproductive tracts is elusive. Herein, we report that after serial treatments with pregnant mare's serum gonadotrophin (PMSG) with or without anti-PMSG (AP), mice could regulate the distribution of ERα and PR in the murine ovary, oviduct and uterus and the level of estradiol in serum. ERα and PR regulation by PMSG and anti-PMSG was estrous cycle-dependent and critical for promoting the embryo-implantation period. Furthermore, our results suggested that AP-42 h treatment is more effective than the other treatments. In contrast, other treatment groups also affected the distribution of ERα and PR in mouse reproductive tracts. Thus, we found that anti-PMSG has the potential to restore the distribution of ERα and PR, which could effectively reduce the negative impact of residual estrogen caused by the normal superovulation effect of PMSG in mice.

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Progesterone Receptor, Estrogen Receptor α, and the Type II Glucocorticoid Receptor Are Coexpressed in the Same Neurons of the Ovine Preoptic Area and Arcuate Nucleus: A Triple Immunolabeling Study1

Biology of Reproduction ◽

10.1095/biolreprod.102.005066 ◽

2002 ◽

Vol 67 (5) ◽

pp. 1605-1612 ◽

Cited By ~ 30

Author(s):

Laurence Dufourny ◽

Donal C. Skinner

Keyword(s):

Estrogen Receptor ◽

Progesterone Receptor ◽

Glucocorticoid Receptor ◽

Arcuate Nucleus ◽

Preoptic Area ◽

Estrogen Receptor Α ◽

Type Ii

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Immunohistochemical biomarker expression in gastro-entero-pancreatic neuroendocrine tumors at ENETS Centre of Excellence, University Hospital Zurich, Switzerland.

Journal of Clinical Oncology ◽

10.1200/jco.2020.38.15_suppl.e15673 ◽

2020 ◽

Vol 38 (15_suppl) ◽

pp. e15673-e15673

Author(s):

Silvia Duschek ◽

Juliane Friemel ◽

Alessandra Curioni Fontecedro ◽

Alexander Rheinhard Siebenhuener

Keyword(s):

Estrogen Receptor ◽

Progesterone Receptor ◽

Tissue Microarray ◽

Neuroendocrine Tumors ◽

Estrogen Receptor Α ◽

University Hospital ◽

Pancreatic Neuroendocrine Tumors ◽

Biological Behaviour ◽

Primary Tumors ◽

Therapy Targets

e15673 Background: Gastro-entero-pancreatic neuroendocrine tumors (GEP-NET) are a heterogeneous tumor entity with respect to biological behaviour and prognosis. Definition of new predictive and prognostic biomarkers as well as new therapy targets are of upmost clinical interest. Methods: At the ENETS CoE Zurich tissue microarray (TMA) blocks were constructed with 147 tissue samples of tumors diagnosed from 2000 to 2017, including primary tumors and metastasis. Tissue microarray sections were immunostained for SOX-9, SOX-10, SSTR-2 +, PDL-1, thyroid transcription factor 1 (TTF1), estrogen receptor-α (ER-α) and -β (ER-β), progesterone receptor (PR), androgen receptor (AR), BRAF and HER2. Results: In total 270 patients were screened between 2000 - 2017 and 92 patient’s material were sufficient for TMA analysis. Subgroup analysis showed 32 pancreatic, 37 ileum, 10 duodenum, 7 appendix, 3 colorectal, 3 gastric and 1 NET of gallbladder. 50% were male, the median age 63.5 years (range 19-88y). AR, ER-β, TTF1, PDL-1 and SOX10 was only present in a small number of NETs. HER2 and BRAF were negative in all samples. We found ER-α expression in 27.2%, 30% were PR positive and 67.4% showed SOX9 expression. Conclusions: We identified a frequent expression of new markers as SOX9, progesterone receptor and estrogen receptor-α in a broad amount of samples and a potential correlation with tumor grade. This finding might implicate a prognostic or predictive value of these markers, as well as it reveals new potential therapy targets for GEP-NETs. Therefore, further analyses are planned.

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