A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections

2002 ◽  
Vol 9 (3) ◽  
pp. 214-219 ◽  
Author(s):  
Sathyanesan Samuel Newton ◽  
Antonia Dow ◽  
Rose Terwilliger ◽  
Ronald Duman
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Simplified Method ◽  
Fresh Frozen

Developmental expression of somatostatin in mouse brain. II. In situ hybridization

1990 ◽  
Vol 53 (1) ◽  
pp. 26-39 ◽  
Author(s):  
Caterina Bendotti ◽  
Christine Hohmann ◽  
Gianluigi Forloni ◽  
Roger Reeves ◽  
Joseph T. Coyle ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Developmental Expression

Anatomical characterization of the neuropeptide S system in the mouse brain by in situ hybridization and immunohistochemistry

2011 ◽  
Vol 519 (10) ◽  
pp. 1867-1893 ◽  
Author(s):  
Stewart D. Clark ◽  
Dee M. Duangdao ◽  
Stefan Schulz ◽  
Li Zhang ◽  
Xiaobin Liu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Neuropeptide S ◽  
Anatomical Characterization

scholarly journals In situ hybridization histochemistry localization of interleukin-3 mRNA in mouse brain

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 137-140
Author(s):  
WL Farrar ◽  
M Vinocour ◽  
JM Hill
Keyword(s):  
In Situ Hybridization ◽  
Mouse Brain ◽  
Neuronal Cell Body ◽  
Northern Analysis ◽  
Molecular Evidence ◽  
Hybridization Histochemistry ◽  
Interleukin 3 ◽  
In Situ Hybridization Histochemistry ◽  
The Brain

The hematopoietic growth factor interleukin-3 (IL-3) promotes the proliferation and maturation of pluripotent myeloid progenitor cells. In the immune system, IL-3 is synthesized by mitogen or antigen- stimulated T lymphocytes. We demonstrate the expression of IL-3 mRNA in mouse brain by in situ hybridization histochemistry and Northern blot analysis. The IL-3 mRNA is localized in discrete areas of the brain and can be found in neuronal cell body and astrocytes. Northern analysis of cerebellar RNA, compared with mRNA extracted from WEHI-3 cells, showed a single hybridization band, approximately 1.2 kb, suggesting similar processing between brain and myeloid cells. The molecular evidence and previous observations of IL-3-like biologic activity found in the brain suggest a potential role for IL-3 in the neurobiology of the CNS.

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