Physical characterization of the stratum corneum of an in vitro human skin equivalent produced by tissue engineering and its comparison with normal human skin by ATR-FTIR spectroscopy and thermal analysis (DSC)

1999 ◽  
Vol 1439 (3) ◽  
pp. 341-352 ◽  
Author(s):  
Roxane Pouliot ◽  
Lucie Germain ◽  
François A. Auger ◽  
Nathalie Tremblay ◽  
Julianna Juhasz
Keyword(s):  
Thermal Analysis ◽  
Tissue Engineering ◽  
Stratum Corneum ◽  
Ftir Spectroscopy ◽  
Human Skin ◽  
Normal Human Skin ◽  
Normal Human ◽  
Human Skin Equivalent

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

Different patterns of in vitro acantholysis in normal human skin samples explanted from different sites of the body

1998 ◽  
Vol 37 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Vincenzo Ruocco ◽  
Sarah Brenner ◽  
Eleonora Ruocco ◽  
Francesca De Angelis ◽  
Maria Luisa Lombardi
Keyword(s):  
Human Skin ◽  
The Body ◽  
Normal Human Skin ◽  
Normal Human ◽  
Skin Samples

Asiaticoside enhances normal human skin cell migration, attachment and growth in vitro wound healing model

Phytomedicine ◽  
2012 ◽  
Vol 19 (13) ◽  
pp. 1223-1227 ◽  
Author(s):  
Jeong-Hyun Lee ◽  
Hye-Lee Kim ◽  
Mi Hee Lee ◽  
Kyung Eun You ◽  
Byeong-Ju Kwon ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Human Skin ◽  
Normal Human Skin ◽  
Skin Cell ◽  
Normal Human ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Immunoelectron Microscopic Characterization of Human Dermal Lymphatic Microvascular Endothelial Cells: Differential Expression of CD31, CD34, and Type IV Collagen with Lymphatic Endothelial Cells vs Blood Capillary Endothelial Cells in Normal Human Skin, Lymphangioma, and Hemangioma In Situ

1998 ◽  
Vol 46 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Birthe Sauter ◽  
Dagmar Foedinger ◽  
Barbara Sterniczky ◽  
Klaus Wolff ◽  
Klemens Rappersberger
Keyword(s):  
Endothelial Cells ◽  
Human Skin ◽  
Type Iv Collagen ◽  
Microvascular Endothelial Cells ◽  
Lymphatic Endothelial Cells ◽  
Normal Human Skin ◽  
Type Iv ◽  
Normal Human ◽  
Microvascular Endothelial

We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial cells. With blood microvascular endothelial cells, we observed uniform labeling of the luminal cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial cells in normal human skin and in lymphangiomas displayed, in addition to a luminal labeling, pronounced expression of CD31 and CD34 along the abluminal cell membranes. Moreover, CD31 was preferentially detected within intercellular junctions. The expression of CD34 was mostly confined to abluminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial cells.

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