Integrated biological and chemical control of damping-off caused by Rhizoctonia solani using Bacillus subtilis RB14-C and flutolanil

2001 ◽  
Vol 91 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Maki Kondoh ◽  
Mitsuyo Hirai ◽  
Makoto Shoda
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Chemical Control ◽  
Damping Off

scholarly journals Biocontrol of damping-off of zucchini squash seedlings with Bacillus subtilis QST 713

2021 ◽  
Vol 39 (2) ◽  
Author(s):  
Alma Rosa Solano-Báez ◽  
Santos Gerardo Leyva-Mir ◽  
Rosalía Núñez-Pastrana ◽  
Andrés Quezada-Salinas ◽  
Guillermo Márquez-Licona
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Phytophthora Capsici ◽  
Damping Off

<em>Bacillus subtilis</em> presenta actividad antagónica contra fitopatógenos. En el presente estudio, se identificaron los hongos asociados al ahogamiento en plántulas de calabacita y se evaluó la efectividad de la cepa QST 713 de <em>B. subtilis</em> ante la infección combinada de los hongos aislados. Los patógenos se aislaron de plántulas de calabacita con síntomas de ahogamiento. Se inocularon plántulas de calabacita var. Grey zucchini con propágulos de tres patógenos a una concentración de 4×105 UFC de cada patógeno. Se evaluó la efectividad de <em>B. subtilis</em> (2, 4 y 6×107 UFC mL-1) y se comparó con metil tiofanato + propamocarb clorhidrato (preventiva y curativa). La incidencia se evaluó a los tres, seis, nueve y 12 días (ddi). Se aislaron e identificaron tres hongos de 100 aislamientos: <em>Phytophthora capsici</em> (62%), <em>Rhizoctonia solani</em> (26%) y <em>Fusarium oxysporum</em> (12%). Se observó la eficiencia de <em>B. subtilis</em>, con una reducción en la incidencia de la enfermedad conforme se incrementó la concentración. Doce días después de la inoculación, la incidencia del ahogamiento en los tratamientos con <em>B. subtilis</em> varió de 18.3 a 41%. El tratamiento de <em>B. subtilis</em> (6×107 UFC mL-1) fue estadísticamente igual a metil tiofanato + propamocarb clorhidrato (curativo). La cepa QST 713 de<em> B. subtilis</em> controló un 81.7% el ahogamiento de plántulas de calabacita, aplicada de manera preventiva a una concentración de 6×107 UFC mL-1.

scholarly journals EVALUASI LIMA JENIS INNER CARRIER DAN FORMULASI BACILLUS SUBTILIS UNTUK PENGENDALIAN HAWAR PELEPAH JAGUNG (RHIZOCTONIA SOLANI KUHN)

2016 ◽  
Vol 15 (2) ◽  
pp. 164
Author(s):  
Amran Muis ◽  
Nurasiah Djaenuddin ◽  
Nurnina Nonci
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Plant Pathogens ◽  
Control Method ◽  
Biological Control Agents ◽  
Damping Off ◽  
Fresh Weight ◽  
Corn Flour ◽  
Alternative Control ◽  
Antagonistic Microorganisms

Evaluation of five inner carriers and Bacillus subtilis formulation to control banded leaf and sheath blight (Rhizoctonia solani Kuhn). One alternative control method against plant pathogens is the use of antagonistic microorganisms, such as Bacillus subtilis. The use of the antagonistic bacteria on corn especially in Indonesia is still lack. The objective of this research was to evaluate some inner carrier and to make formulated antagonistic B. subtilis to be used as biological control agents on corn diseases. This research consists of laboratory and greenhouse activities. The laboratory activities consist of B. subtilis biomass production, formulation of B. subtilis, and evaluation of five types of inner carrier. In the greenhouse, testing the formulation B. subtilis with talc as an inner carrier, which is compared with the treatment solution of B. subtilis, nordox, metalaxyl fungicides. The data collected in this study were percentage of germination, damping off due to pathogen R. solani, plant height, plant fresh weight, and percentages of R. solani incidence on 14 DAP. The results showed that talc powder and corn flour were the best inner carrier to be used in sorage formulation of antagonistic Bacillus. Formulated Bacillus subtilis TM4 showed no negative affect on seed germination and able to suppress the development of R. solani in greenhouse.

scholarly journals Induction of Soil Suppressiveness Against Rhizoctonia solani by Incorporation of Dried Plant Residues into Soil

2006 ◽  
Vol 96 (12) ◽  
pp. 1372-1379 ◽  
Author(s):  
Masahiro Kasuya ◽  
Andriantsoa R. Olivier ◽  
Yoko Ota ◽  
Motoaki Tojo ◽  
Hitoshi Honjo ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Mycelial Growth ◽  
Inhibitory Effect ◽  
Volatile Substance ◽  
Disease Suppression ◽  
Plant Residues ◽  
Infested Soil ◽  
Antagonistic Bacteria ◽  
Damping Off ◽  
Soil Suppressiveness

Suppressive effects of soil amendment with residues of 12 cultivars of Brassica rapa on damping-off of sugar beet were evaluated in soils infested with Rhizoctonia solani. Residues of clover and peanut were tested as noncruciferous controls. The incidence of damping-off was significantly and consistently suppressed in the soils amended with residues of clover, peanut, and B. rapa subsp. rapifera ‘Saori’, but only the volatile substance produced from water-imbibed residue of cv. Saori exhibited a distinct inhibitory effect on mycelial growth of R. solani. Nonetheless, disease suppression in such residue-amended soils was diminished or nullified when antibacterial antibiotics were applied to the soils, suggesting that proliferation of antagonistic bacteria resident to the soils were responsible for disease suppression. When the seed (pericarps) colonized by R. solani in the infested soil without residues were replanted into the soils amended with such residues, damping-off was suppressed in all cases. In contrast, when seed that had been colonized by microorganisms in the soils containing the residues were replanted into the infested soil, damping-off was not suppressed. The evidence indicates that the laimosphere, but not the spermosphere, is the site for the antagonistic microbial interaction, which is the chief principle of soil suppressiveness against Rhizoctonia damping-off.

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