MP044 Activity of moxifloxacin (MXF) against Bacteroides fragilis in an in-vitro pharmacokinetic/pharmacodynamic (PK/PD) model in single and mixed cultures

2003 ◽  
Vol 293 ◽  
pp. 266
Keyword(s):  
Bacteroides Fragilis ◽  
Mixed Cultures ◽  
Single And Mixed Cultures

scholarly journals Activity of Moxifloxacin, Imipenem, and Ertapenem against Escherichia coli, Enterobacter cloacae, Enterococcus faecalis, and Bacteroides fragilis in Monocultures and Mixed Cultures in anIn VitroPharmacokinetic/Pharmacodynamic Model Simulating Concentrations in the Human Pancreas

2012 ◽  
Vol 56 (12) ◽  
pp. 6434-6436 ◽  
Author(s):  
Sabine Schubert ◽  
Axel Dalhoff
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Necrotizing Pancreatitis ◽  
Bacteroides Fragilis ◽  
Enterobacter Cloacae ◽  
Pharmacodynamic Model ◽  
Mixed Cultures ◽  
Human Pancreas ◽  
Content Type

ABSTRACTThe activities of moxifloxacin, imipenem, and ertapenem against pathogens causing severe necrotizing pancreatitis were studied in anin vitropharmacokinetics/pharmacodynamics (PK/PD) model.Escherichia coli,Enterobacter cloacae,Enterococcus faecalis, andBacteroides fragiliswere exposed in monocultures and mixed cultures to concentrations of the three agents comparable to those in the human pancreas. Moxifloxacin was more active than the two carbapenems in monocultures and mixed cultures, reducing the numbers of CFU more drastically and more rapidly.

scholarly journals Antioxidant Activity of Endophytic Fungi Isolated from Turmeric Flowers

2020 ◽  
Vol 12 (2) ◽  
pp. 268-273
Author(s):  
Eris Septiana ◽  
Yadi Yadi ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
The Body ◽  
Mixed Cultures ◽  
Additional Information ◽  
Single And Mixed Cultures ◽  
In Vitro Antioxidant Activity

Antioxidants are compounds needed by the body to fight free radicals that can cause adverse effects on health. All parts of turmeric plant have antioxidant activity, so does the flower. It is thought that the endophytic fungi from turmeric flowers also have antioxidant activity and no studies have reported it yet. The purpose of this study was to study the antioxidant activity of single and mixed cultures of endophytic fungi isolated from turmeric flowers. In vitro antioxidant activity assay was conducted using the DDPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging method by measuring its absorption using a UV-Vis spectrophotometer at a wavelength of 517 nm. The extract of single and mixed cultures of endophytic fungi isolated from turmeric flowers showed antioxidant activity. The combination of fungal cultures can increase the antioxidant activity of a single culture. This study is the first to report that the combination of endophytic fungi from turmeric flowers can increase its antioxidant activity. Therefore, the presence of the endophytic fungi in turmeric flowers that have antioxidant activity can provide additional information so that the utilization of turmeric flowers as a source of natural antioxidants will increase.

scholarly journals In vitro activity of piperacillin/tazobactam and ertapenem against Bacteroides fragilis and Escherichia coli in pure and mixed cultures

2007 ◽  
Vol 56 (6) ◽  
pp. 798-802 ◽  
Author(s):  
Kênia Valéria dos Santos ◽  
Cláudio Galuppo Diniz ◽  
Simone Cristina Coutinho ◽  
Ana Carolina Morais Apolônio ◽  
Luciana Geralda de Sousa-Gaia ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacteroides Fragilis ◽  
Mixed Cultures ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
In Vitro Activity ◽  
E Coli ◽  
Agar Dilution ◽  
Time Kill

Ertapenem and piperacillin/tazobactam are β-lactam antibiotics with a broad spectrum of activity used for the treatment of mixed infections in which Bacteroides fragilis and Escherichia coli play an important aetiological role. In this study, the activities of piperacillin/tazobactam and ertapenem (MIC and time–kill kinetics) against these bacteria were compared. MICs were determined by the agar dilution method, and the time and slope of time–kill curves were analysed. In the in vitro pharmacodynamic assays, pure and mixed cultures of E. coli and B. fragilis were exposed to peak concentrations of ertapenem (8.0 μg ml−1) and piperacillin/tazobactam (64.0/8.0 μg ml−1) for 48 h. Treatment with ertapenem reduced the viability of E. coli and/or B. fragilis by 3 logs in all experiments, whereas piperacillin/tazobactam only affected the viability of B. fragilis. Both drugs exhibited their fastest rates of killing when bacteria were grown in mixed cultures. According to the results, ertapenem exhibited activity similar to that of piperacillin/tazobactam against B. fragilis alone or in mixed culture. However, ertapenem exhibited a markedly higher activity against E. coli alone or in combination with B. fragilis relative to piperacillin/tazobactam.

In Vitro Inhibition of the Growth of Salmonella typhimurium and Escherichia coli 0157:H7 by Bacteria Isolated from the Cecal Contents of Adult Chickens

1991 ◽  
Vol 54 (7) ◽  
pp. 496-501 ◽  
Author(s):  
ARTHUR HINTON ◽  
GEORGE E. SPATES ◽  
DONALD E. CORRIER ◽  
MICHAEL E. HUME ◽  
JOHN R. DELOACH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Salmonella Typhimurium ◽  
Mixed Cultures ◽  
Enterococcus Durans ◽  
E Coli ◽  
Pure Cultures ◽  
In Vitro Inhibition ◽  
Lactic Acids

A Veillonella species and Enterococcus durans were isolated from the cecal contents of adult broilers. Mixed cultures of Veillonella and E. durans inhibited the growth of Salmonella typhimurium and Escherichia coli 0157:H7 on media containing 2.5% lactose (w/v). The growth of S. typhimurium or E. coli 0157:H7 was not inhibited by mixed cultures containing Veillonella and E. durans on media containing only 0.25% lactose or by pure cultures of Veillonella or E. durans on media containing either 0.25% or 2.5% lactose. The mixed cultures of Veillonella and E. durans produced significantly (P<0.05) more acetic, propionic, and lactic acids in media containing 2.5% lactose than in media containing 0.25% lactose. The inhibition of the enteropathogens was related to the production of lactic acid from lactose by the E. durans and the production of acetic and propionic acids from lactic acid by the Veillonella.

scholarly journals In vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.

1996 ◽  
Vol 40 (9) ◽  
pp. 2232-2235 ◽  
Author(s):  
H M Wexler ◽  
E Molitoris ◽  
D Molitoris ◽  
S M Finegold
Keyword(s):  
Antimicrobial Activity ◽  
Clinical Laboratory ◽  
Anaerobic Bacteria ◽  
Bacteroides Fragilis ◽  
National Committee ◽  
Dilution Technique ◽  
Agar Dilution ◽  
Group Species ◽  
Clostridium Spp

The antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the National Committee for Clinical Laboratory Standards-approved Wadsworth agar dilution technique. The species tested included Bacteroides fragilis (n = 91), other members of the B. fragilis group (n = 130), Campylobacter gracilis (n = 15), other Bacteroides spp. (n = 16), Prevotella spp. (n = 49), Porphyromonas spp. (n = 15), Fusobacterium spp. (n = 62), Bilophila wadsworthia (n = 24), Sutterella wadsworthensis (n = 21), Clostridium spp. (n = 61), Peptostreptococcus spp. (n = 38), and gram-positive non-spore-forming rods (n = 35). Trovafloxacin inhibited all strains of B. fragilis at < or = 0.5 microgram/ml, 99% of other B. fragilis group species at < or = 2 micrograms/ml, and 96% of all anaerobes tested at < or = 2 micrograms/ml.

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