A comparison between monoclonal and policlonal enzyme immunoassay for assessment of H. pylori infection in stool after eradication treatment

A highly sensitive seminested PCR assay to detectHelicobacter pylori DNA in feces was developed. PCR with stool specimens and a novel antigen enzyme immunoassay (EIA) forH. pylori detection in feces were evaluated as diagnostic tools and in follow-up with samples from 63 infected and 37 noninfected persons. Infected individuals received eradication therapy followed by endoscopic follow-up 35 days after the start of treatment. At that time, a second stool specimen was obtained from 55 of these patients. Before eradication, the sensitivity of PCR was 93.7% and that of EIA 88.9%. Specificities were 100 and 94.6%, respectively. Of the 55 follow-up specimens, 41 originated from patients from whom H. pylori had been eradicated. Of these, 21 were still positive by PCR and 13 were positive by EIA, indicating that 1 month may be too short a period for follow-up evaluation of stool specimens by these tests.

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A novel enzyme immunoassay for the direct detection of H. pylori antigens in stool

Gastroenterology ◽

10.1016/s0016-5085(08)82443-2 ◽

2001 ◽

Vol 120 (5) ◽

pp. A492-A493 ◽

Cited By ~ 4

Author(s):

Eva Haindl ◽

Helmut Benesch ◽

Andreas Finck ◽

Verena Muehistein ◽

Andreas Leodolter ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Direct Detection ◽

H Pylori

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Detection of Helicobacter pylori Antibodies in a Pediatric Population: Comparison of Three Commercially Available Serological Tests and One In-House Enzyme Immunoassay

Journal of Clinical Microbiology ◽

10.1128/jcm.37.10.3328-3331.1999 ◽

1999 ◽

Vol 37 (10) ◽

pp. 3328-3331 ◽

Cited By ~ 18

Author(s):

Bengt Sunnerstam ◽

Torbjörn Kjerstadius ◽

Lillemor Jansson ◽

Johan Giesecke ◽

Mats Bergström ◽

...

Keyword(s):

Helicobacter Pylori ◽

Enzyme Immunoassay ◽

Reference Method ◽

False Positives ◽

Serological Response ◽

Serum Samples ◽

False Negatives ◽

Serological Tests ◽

Predictive Values ◽

H Pylori

A serum immunoglobulin G enzyme immunoassay (EIA) forHelicobacter pylori antibodies already in use in adults was evaluated with 99 pediatric serum samples to determine its usefulness for the study of H. pylori disease in children. The reference method used was either the 13C-urea breath test or a biopsy culture of gastric mucosa. In children, an EIA cutoff of 0.35 absorbancy unit yielded sensitivity, specificity, and positive and negative predictive values of 93, 97, 93, and 97%, respectively. The cutoff recommended when this EIA was published for use in adults was 0.70 absorbancy unit (H. Gnarpe, P. Unge, C. Blomqvist, and S. Mäkitalo, APMIS 96:128–132, 1988). Another subset of 169 serum samples taken from children was analyzed by four serological tests in order to compare the performance of the in-house EIA with the Pyloriset, HM-CAP, and Helico-G kits. For the 169 samples, 10 (5.9%) false-positives and no false-negatives occurred with the Helico-G, 3 (1.8%) false-positives and no false-negatives occurred with the Pyloriset, and 3 (1.8%) false-positives and 1 (0.6%) false-negative occurred with the HM-CAP. For the 169 samples, 1 (0.6%) false-positive and no false-negatives occurred with the in-house EIA. Serological detection of H. pylori antibodies with our EIA seems to be valuable in diagnosing H. pylori infection in children, but only if a lowered, specific pediatric cutoff is established. The commercial kits, particularly the Helico-G, seem to overdiagnose pediatric H. pylori infection. A positive serological test for H. pylori infection, particularly for children, needs to be confirmed by a reference method because of the possibility of spontaneous eradication of infection, with a lingering serological response.

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Prevalence rate of Helicobacter pylori infection among population of Ryazan region

I P Pavlov Russian Medical Biological Herald ◽

10.23888/pavlovj201927135-40 ◽

2019 ◽

Vol 27 (1) ◽

pp. 35-40

Author(s):

Tatyana V. Zhestkova ◽

Mikhail A. Butov ◽

Yulian Yu. Lymar ◽

Sergey V. Papkov

Keyword(s):

Helicobacter Pylori ◽

Enzyme Immunoassay ◽

Serological Test ◽

Adult Population ◽

Test System ◽

Helicobacter Pylori Infection ◽

Quantitative Detection ◽

Serological Tests ◽

H Pylori

Aim. Determination of the prevalence of Helicobacter pylori (H. pylori) infection among the population of the Ryazan region. Materials and Methods. 833 individuals (809 adults and 24 children) were examined for presence of IgG class antibodies using the enzyme immunoassay (2017-2018). The criteria for inclusion into the study were: a desire of a patient to undergo examination for the presence of antibodies to H. pylori in blood. Criteria for exclusion: past treatment for helicobacteriosis. The presence of helicobacteriosis was determined by enzyme immunoassay for quantitative detection of IgG class antibodies (anti-H. pylori IgG) using BCM Diagnostics Helicobacter pylori IgG (USA) test system and for qualitative determination of IgG antibodies to H. pylori in blood serum on IMMULITE 2000 (Germany; test IMMULITE 2000 H. pylori IgG). Sensitivity of the used test systems was 95.0%, specificity 98.0%. Results. High contamination of adult residents of Ryazan with H. pylori 65.6% was found (70.6% of males, 64.4% of females). Prevalence of H. pylori infection among adults in 2017 was 64.4% and in 2018 70.2%, however, the observed increase in the number of infected individuals was not statistically significant (p0.05). The highest prevalence of H. pylori infection was observed in individuals 40 years of age (67.2%). Gender-related differences in the prevalence of Helicobacter pylori infection were revealed in individuals of 40 years and older. H. pylori infection in males of 40 years was 75.2%, against 65.5% in females of the same age (p0.05). In children of 4-16 years, the share of individuals with positive serological test with anti-H. pylori IgG reached 20.8%. All H. pylori infected children were above 9 years of age. Individuals with positive serological tests received consultation of a gastroenterologist, and on indications underwent additional examination with administration of eradication treatment. In patients with indefinite results the examination was repeated after a week and/or the presence of H. pylori antigen in feces was determined. Conclusion. The data obtained indicate a high level of infection with H. pylori in the adult population in the Ryazan region 65.6%. The incidence of detection of anti-H. pylori IgG in the population was maximal in individuals 40 years (67.2%).

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Evaluation of Three Commercial Serological Tests with Different Methodologies To Assess Helicobacter pyloriInfection

Journal of Clinical Microbiology ◽

10.1128/jcm.37.12.4150-4152.1999 ◽

1999 ◽

Vol 37 (12) ◽

pp. 4150-4152 ◽

Cited By ~ 19

Author(s):

A. van der Ende ◽

R. W. M. van der Hulst ◽

P. Roorda ◽

G. N. J. Tytgat ◽

J. Dankert

Keyword(s):

Helicobacter Pylori ◽

Enzyme Immunoassay ◽

Immunosorbent Assay ◽

Solid Phase ◽

Enzyme Linked Immunosorbent Assay ◽

Serological Tests ◽

H Pylori ◽

Chemiluminescent Enzyme Immunoassay

The sera of 142 Helicobacter pylori-positive and 32H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.

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EXPERIENCE OF USING THE TEST SYSTEM «GASTROPANEL» IN THE PREVENTIVE MEDICAL EXAMINATION OF WORKFORCE IN JSC RZD

10.31089/978-5-6042929-2-1-2021-1-173-176 ◽

2021 ◽

Author(s):

A.O. Devyatko ◽

◽

T.A. Noskova ◽

N.V. Shevchenko

Keyword(s):

Helicobacter Pylori ◽

Gastric Mucosa ◽

Enzyme Immunoassay ◽

Test System ◽

Medical Examination ◽

Pepsinogen I ◽

Non Invasive ◽

H Pylori ◽

Pepsinogen Ii

Abstract: Non-invasive diagnosis of the gastric mucosa was carried out in 181 people in the preventive medical examination of workforce in JSC RZD. A set of diagnostics from Vector-Best (Novosibirsk, Russia) was used for enzyme immunoassay of pepsinogen I, pepsinogen II, and immunoglobulins for Helicobacter pylori. The prevalence of H. pylori infection in more than half of the employees, the presence of deviations of biomarkers from the norm in more than one in three employees were revealed. Laboratory signs of atrophy were detected eight times less frequently than signs of inflammation of the gastric mucosa, but when infected with H. pylori and increased with age.

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Su1148 Evaluation of the Accuracy of DB-13-04, a Novel Enzyme Immunoassay KIT, for the Detection of Anti-H. pylori IgG Antibody

Gastroenterology ◽

10.1016/s0016-5085(15)31419-0 ◽

2015 ◽

Vol 148 (4) ◽

pp. S-420-S-421

Author(s):

Takahisa Furuta ◽

Mitsushige Sugimoto ◽

Takahiro Uotani ◽

Mihoko Yamade ◽

Shu Sahara ◽

...

Keyword(s):

Enzyme Immunoassay ◽

Igg Antibody ◽

H Pylori

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The rod-to-coccoid body transformation in cultures of Helicobacter pylori

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160686 ◽

1990 ◽

Vol 48 (3) ◽

pp. 626-627

Author(s):

A. R. Crooker ◽

W. G. Kraft ◽

T. L. Beard ◽

M. C. Myers

Keyword(s):

Helicobacter Pylori ◽

Growth Cycle ◽

Negative Bacterium ◽

Body Formation ◽

Coccoid Form ◽

Spiral Form ◽

Prolonged Culture ◽

H Pylori ◽

Upper Gastrointestinal

Helicobacter pylori is a microaerophilic, gram-negative bacterium found in the upper gastrointestinal tract of humans. There is strong evidence that H. pylori is important in the etiology of gastritis; the bacterium may also be a major predisposing cause of peptic ulceration. On the gastric mucosa, the organism exists as a spiral form with one to seven sheathed flagella at one (usually) or both poles. Short spirals were seen in the first successful culture of the organism in 1983. In 1984, Marshall and Warren reported a coccoid form in older cultures. Since that time, other workers have observed rod and coccal forms in vitro; coccoid forms predominate in cultures 3-7 days old. We sought to examine the growth cycle of H. pylori in prolonged culture and the mode of coccoid body formation.

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Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158388 ◽

1990 ◽

Vol 48 (3) ◽

pp. 168-169

Author(s):

M. H. Chestnut ◽

C. E. Catrenich

Keyword(s):

Acridine Orange ◽

Mammalian Cells ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Confocal Laser ◽

Ulcer Disease ◽

Gastroduodenal Disease ◽

H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

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