ISSR Analysis on Genetic Diversity of the 34 Populations of Oryza meyeriana Distributing in Yunnan Province, China

Rice stripe virus (RSV) is one of the most economically important pathogens of rice and is repeatedly epidemic in China, Japan and Korea. The most recent outbreak of RSV in eastern China in 2000 caused significant losses and raised serious concerns. In this paper, we provide a genotyping profile of RSV field isolates and describe the population structure of RSV in China, based on the nucleotide sequences of isolates collected from different geographical regions during 1997–2004. RSV isolates could be divided into two or three subtypes, depending on which gene was analysed. The genetic distances between subtypes range from 0.050 to 0.067. The population from eastern China is composed only of subtype I/IB isolates. In contrast, the population from Yunnan province (southwest China) is composed mainly of subtype II isolates, but also contains a small proportion of subtype I/IB isolates and subtype IA isolates. However, subpopulations collected from different districts in eastern China or Yunnan province are not genetically differentiated and show frequent gene flow. RSV genes were found to be under strong negative selection. Our data suggest that the most recent outbreak of RSV in eastern China was not due to the invasion of new RSV subtype(s). The evolutionary processes contributing to the observed genetic diversity and population structure are discussed.

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Genetic diversity of wild Auricularia auricula-judae revealed by ISSR analysis

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2012.11.011 ◽

2013 ◽

Vol 48 ◽

pp. 199-205 ◽

Cited By ~ 4

Author(s):

Ping Du ◽

Bao-Kai Cui ◽

Chun-Feng Zhang ◽

Yu-Cheng Dai

Keyword(s):

Genetic Diversity ◽

Issr Analysis ◽

Auricularia Auricula

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Genetic Diversity and Natural Selection of Plasmodium vivax Duffy Binding Protein-II From China-Myanmar Border of Yunnan Province, China

Frontiers in Microbiology ◽

10.3389/fmicb.2021.758061 ◽

2021 ◽

Vol 12 ◽

Author(s):

Tian-Qi Shi ◽

Hai-Mo Shen ◽

Shen-Bo Chen ◽

Kokouvi Kassegne ◽

Yan-Bing Cui ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Natural Selection ◽

Genetic Differentiation ◽

Positive Selection ◽

Plasmodium Vivax ◽

Yunnan Province ◽

Duffy Binding Protein ◽

Synonymous Mutations ◽

Selection Of

Malaria incidence has declined dramatically over the past decade and China was certified malaria-free in 2021. However, the presence of malaria in border areas and the importation of cases of malaria parasites are major challenges for the consolidation of the achievements made by China. Plasmodium vivax Duffy binding protein (PvDBP) performs a significant role in erythrocyte invasion, and is considered a promising P. vivax vaccine. However, the highly polymorphic region of PvDBP (PvDBP-II) impedes the development of blood-stage vaccine against P. vivax. In this study, we investigated the genetic diversity and natural selection of PvDBP-II among 124 P. vivax isolates collected from the China-Myanmar border (CMB) in Yunnan Province, China, during 2009–2011. To compare genetic diversity, natural selection, and population structure with CMB isolates, 85 pvdbp-II sequences of eastern Myanmar isolates were obtained from GenBank. In addition, global sequences of pvdbp-II were retrieved from GenBank to establish genetic differentiation relationships and networks with the CMB isolates. In total, 22 single nucleotide polymorphisms reflected in 20 non-synonymous and two synonymous mutations were identified. The overall nucleotide diversity of PvDBP-II from the 124 CMB isolates was 0.0059 with 21 haplotypes identified (Hd = 0.91). The high ratio of non-synonymous to synonymous mutations suggests that PvDBP-II had evolved under positive selection. Population structure analysis of the CMB and eastern Myanmar isolates were optimally grouped into five sub-populations (K = 5). Polymorphisms of PvDBP-II display that CMB isolates were genetically diverse. Mutation, recombination, and positive selection promote polymorphism of PvDBP-II of P. vivax population. Although low-level genetic differentiation in eastern Myanmar was identified along with the more effective malaria control measures, the complexity of population structure in malaria parasites has maintained. In conclusion, findings from this study advance knowledge of the understanding of the dynamic of P. vivax population, which will contribute to guiding the rational design of a PvDBP-II based vaccine.

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Genetic diversity among flue-cured tobacco cultivars on the basis of AFLP markers

Czech Journal of Genetics and Plant Breeding ◽

10.17221/15/2009-cjgpb ◽

2009 ◽

Vol 45 (No. 4) ◽

pp. 155-159 ◽

Cited By ~ 1

Author(s):

X.Z. Liu ◽

C.S. He ◽

Y.M. Yang ◽

H.Y. Zhang

Keyword(s):

Genetic Diversity ◽

Yunnan Province ◽

Southwest China ◽

Germplasm Conservation ◽

Research Centre ◽

Aflp Data ◽

Breeding Research ◽

Polymorphic Bands ◽

Relationship Of ◽

The Relationship

AFLP analyses were used to assess the genetic similarity among selected accessions at the South China Tobacco Breeding Research Centre (Yunnan province, Southwest China). 154 AFLP polymorphic fragments out of 561 fragments were used to assess the genetic diversity among 28 tobacco accessions. The average number of polymorphic bands per AFLP primer pair was 15.4. AFLPs seemed to be an effective classification tools for germplasm conservation and breeding. Limited genetic variation was detected within this group of accessions. The relationship of cultivars was estimated by cluster analysis based on AFLP data.

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