Development of an eco-friendly and fast HPLC method for quantitative analysis of four nucleosides in Cordyceps and related products

2021 ◽  
Vol 19 (12) ◽  
pp. 954-960
Author(s):  
Zheng-Ming QIAN ◽  
Zi WU ◽  
Qi HUANG ◽  
Chuan-Xi WANG ◽  
Guo-Yin TAN ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Hplc Method

scholarly journals A A RAPID AND SENSITIVE RP-HPLC METHOD FOR THE QUANTITATIVE ANALYSIS OF EMPAGLIFLOZIN IN BULK AND PHARMACEUTICAL DOSAGE FORM

2019 ◽  
pp. 60-65
Author(s):  
MADHURIMA BASAK ◽  
Santhosh Reddy Gouru ◽  
Animesh Bera ◽  
Krishna veni Nagappan
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Limit Of Quantitation ◽  
Bulk Drug

Objective: The present study aims at developing an accurate precise, rapid and sensitive Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) method for assessing Empagliflozin in bulk drug and in the pharmaceutical dosage form. Methods: The proposed method employs a Reverse Phase Shim Pack C18 column (250 mm × 4.6 mm id; 5 µm) using a mobile phase comprising of acetonitrile and water in the ratio of 60:40 v/v flushed at a flow rate of 1 ml/min. The eluents were monitored at 223 nm. Results: Empagliflozin was eluted at a retention time of 5.417 min and established a co-relation co-efficient (R2>0.999) over a concentration ranging from 0.0495-100µg/ml. Percentage recovery was obtained between 98-102% which indicated that the method is accurate. The Limit of Detection (LOD) and Limit of Quantitation (LOQ) were found at 0.0125µg/ml and 0.0495µg/ml, respectively. Conclusion: An RP-HPLC method which was relatively simple, accurate, rapid and precise was developed and its validation was performed for the quantitative analysis of empagliflozin in bulk and tablet dosage form (10 and 25 mg) in accordance to International Conference of Harmonization (ICH) Q2 (R1) guidelines. The proposed method may aid in routinely analyzing empagliflozin in pharmaceuticals.

Development and Validation of an HPLC Method for the Impurity and Quantitative Analysis of Etoricoxib

2003 ◽  
Vol 26 (15) ◽  
pp. 2551-2566 ◽  
Author(s):  
Robert Hartman ◽  
Ahmed Abrahim ◽  
Andrew Clausen ◽  
Bing Mao ◽  
Louis S. Crocker ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Hplc Method ◽  
Development And Validation

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

Quantitative analysis of some pharmaceuticals by the HPLC method

2009 ◽  
Vol 64 (4) ◽  
pp. 210-213
Author(s):  
G. B. Golubitskii ◽  
V. M. Ivanov
Keyword(s):  
Quantitative Analysis ◽  
Hplc Method

scholarly journals A Validated HPLC Method for the Quantitative Analysis of Trans-Resveratrol and Trans-Piceid in Hungarian Wines

2005 ◽  
Vol 43 (9) ◽  
pp. 445-449 ◽  
Author(s):  
L. Mark ◽  
M. S. P. Nikfardjam ◽  
P. Avar ◽  
R. Ohmacht
Keyword(s):  
Quantitative Analysis ◽  
Hplc Method

scholarly journals HPLC Quantitative Analysis of Main Stilbenes and Flavones in Different Parts ofMatteuccia struthiopteris

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Shubei Li ◽  
Dong Zhang ◽  
Lan Yang ◽  
Yujie Li ◽  
Xiaoxin Zhu ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Linear Regression ◽  
Phosphoric Acid ◽  
Flow Rate ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Hplc Method ◽  
Regression Coefficients ◽  
The Mean ◽  
Different Parts

A simple and accurate HPLC-UV method was developed for the simultaneous quantitative analysis of main stilbenes and flavones in different parts (fronds, rhizomes, and frond bases) ofM. struthiopteris. The chromatographic separation was performed on a Kromasil C18 column (4.6 mm × 250 mm, 5 μm) with the mobile phase of MeOH-H2O (including 0.1% phosphoric acid) using a gradient elution at the flow rate of 1.0 mL min−1and UV detection at 295 nm. The method was validated by specificity, linearity, accuracy (recovery), and precision tests (repeatability, intra- and interday). For all the six compounds, the linear regression coefficients ranged from 0.9958 to 0.9998 within the test ranges; intra- and interday precisions were<2% and the mean recoveries ranged from 98.09 to 103.56%. The amount of these compounds in the frond bases was almost the same as in the rhizomes but much higher than that in the fronds. The results indicate that the HPLC method developed was appropriate for the analysis of the six compounds in different parts (fronds, rhizomes, and frond bases) ofM. struthiopteris.

scholarly journals Comparative Study on Quantification of Total Catechins Using UV-Vis Spectrophotometric Method and High Performance Liquid Chromatography Techniques

2021 ◽  
Vol 37 (1) ◽  
pp. 136-142
Author(s):  
Sitharanjan Kalidass ◽  
Karuppannau Daiyarvijaya ◽  
Rajagopal Raj Kumar
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Method ◽  
Mobile Phase ◽  
High Performance ◽  
Cost Effective ◽  
Hplc Method ◽  
Good Resolution ◽  
Huge Number ◽  
Automated Hplc

Bioavailability of catechinsin wider range of plants was established earlier and it’s utility as medicine against cardiovascular disease, cancer, etc. were also demonstrated. Recent techniques in relation to quantitative analysis of total catechins seem to be laborious and time consuming process to handle huge number of samples. Established spectrophotometry and HPLC methods developed earlier for quantitative determination of total catechins in tea extracts were compared in the present study.UV-Vis spectrophotometric method was adopted to monitor the absorbance at 500 nm of the reaction mixture (catechins and vanillin-H2SO4reagents). Hewlett Packard automated HPLC was used and equipped with Phenomenex Luna 5  phenyl-hexyl column fitted with a Phenomenex guard column. Binary elution was carried out using Mobile phase A (acetic acid and acetonitrile) and Mobile phase B (acetonitrile). Method adopted showed a good resolution of catechin fractions and was found to be accurate for the quantification of total catechins (sum of individual catechins). Results of the both the methods are comparable and variation amongst the two methods ranged between -3.59 and 2.79% among the clones and varied with seasons.As expected UPASI released tea clones exhibited variations in their bioavailability. Lean season edge over the cropping period sampling in terms of total catechins. Results obtained from both the methods are comparable. Two methods can be used for the routine quantitative analysis of total catechins; however, spectrophotometric method found to be simple, rapid and cost effective than that of HPLC method unless individual catechins composition is warranted.

Sign in / Sign up

Export Citation Format

Share Document