scholarly journals Genetic parameters for somatic cell score and production traits in the first three lactations of Chinese Holstein cows

2015 ◽  
Vol 14 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Fu-ping ZHAO ◽  
Gang GUO ◽  
Ya-chun WANG ◽  
Xiang-yu GUO ◽  
Yuan ZHANG ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Genetic Parameters ◽  
Somatic Cell Score ◽  
Holstein Cows ◽  
Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cows

scholarly journals Polymorphisms of the ACSL1 Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2282
Author(s):  
Yan Liang ◽  
Qisong Gao ◽  
Qiang Zhang ◽  
Abdelaziz Adam Idriss Arbab ◽  
Mingxun Li ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Milk Production ◽  
Somatic Cell Score ◽  
Holstein Cows ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chain Acyl ◽  
Chinese Holstein Cows ◽  
Long Chain

Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (ACSL1) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in ACSL1 on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine ACSL1 gene, and six SNPs (5’UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS (p < 0.05). Among these SNPs, SNP 5’UTR-g.20523C>G showed an extremely significant effect on PC and SCS (p < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS (p < 0.01), and also TDMY (p < 0.05). The SNP g.35651G>A had a statistically significant effect on PC (p < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS (p < 0.01) and significantly influenced PC (p < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS (p < 0.05). In summary, the pleiotropic effects of bovine ACSL1 for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows.

scholarly journals Polymorphism in the promoter of <i>TLR4</i> gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

2014 ◽  
Vol 57 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zhixiong Li ◽  
Huilin Zhang ◽  
Hongliang Wang ◽  
Ling Chen ◽  
Lijun Wang ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Milk Production ◽  
Somatic Cell Score ◽  
Upstream Region ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cows ◽  
Tlr4 Gene

Abstract. The objective of this study was to examine the effect of the polymorphism in the promoter of toll-like receptor 4 (TLR4) on mastitis and milk production traits. Two novel single nucleotide polymorphisms (SNPs) G-1539A before 5’-upstream region and G+265C in the 5’-UTR of TLR4 gene were detected, and genetic association analysis was applied on 421 Chinese Holstein cows. The statistical analysis indicated that two SNPs was significantly associated with 305 Days milk yield (P<0.05) and somatic cell score (SCS) respectively (P<0.01). Our results provide evidence that polymorphisms in the promoter of TLR4 are associated with milk production traits and SCS, and may be used as a possible candidate for marker-assisted selection and management in Holstein cattle breeding program.

Anti-mastitis SNV identification of NFκB1 in Chinese Holstein cows and the possible anti-inflammation role of NFκB1/p105 in bovine MECs

2020 ◽  
Vol 52 (11) ◽  
pp. 1191-1201
Author(s):  
Ling Chen ◽  
Rongfu Tian ◽  
Huilin Zhang ◽  
Xiaolin Liu
Keyword(s):  
Somatic Cell ◽  
Mrna Expression ◽  
Protein Content ◽  
Milk Protein ◽  
Holstein Cows ◽  
Chinese Holstein ◽  
Milk Protein Content ◽  
Chinese Holstein Cows ◽  
Anti Inflammation

Abstract NFκB1/p105 is the critical member of the NFκB family which can suppress inflammation, ageing, and cancer when p50/p50 homodimer is formed. Currently, the research about the role of NFκB1/p105 during cow mastitis is limited. Here, we analyzed the correlation of six single-nucleotide variants of the NFκB1 gene with somatic cell count, milk yield, milk fat content, and milk protein content in 547 Chinese Holstein cows, and explored the mRNA expression profiles of the NFκB family and ubiquitin ligases (βTrCP1, βTrCP2, KPC1, KPC2) in LPS-induced bovine mammary epithelial cells (MECs) by transcriptome-Seq. The association analysis showed that cows with SNV2-TT and SNV6-CC in the NFκB1 gene had significantly higher milk protein content (P &lt; 0.05), while cows with SNV5-TT in the NFκB1 gene had significantly lower somatic cell score (SCS), but CC genotype at SNV5 locus was not detected in our Holstein cows. The transcriptome-Seq results demonstrated the mRNA expression of NFκB1 was increased and peaked at 4 h post-induction, while the mRNA expressions of both KPC1 and BCL3 that promote the anti-inflammation function of NFκB1/p105 were decreased in LPS-induced bovine MECs. TNFAIP3, an inhibitor of both degradation and processing of p105 precursor, was markedly increased by more than 3 folds. Furthermore, bta-miR-125b which targets at the 3ʹUTR of TNFAIP3 was reduced by 50%. These results indicated that SNV5-TT of the NFκB1 gene with lower SCS may be an anti-mastitis genotype that could cope with infection more efficiently in Chinese Holstein cows. In addition, the anti-inflammation role of NFκB1/p105 seemed to be inhibited in LPS-induced-bovine MECs because the formation of the p50/p50 homodimer was arrested. This study provides a new perspective to understand the inflammatory mechanism in dairy mastitis.

scholarly journals Polymorphisms in Fatty Acid Desaturase 2 Gene Are Associated with Milk Production Traits in Chinese Holstein Cows

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 671 ◽  
Author(s):  
Mingxun Li ◽  
Qisong Gao ◽  
Mengqi Wang ◽  
Yan Liang ◽  
Yujia Sun ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Milk Production ◽  
Milk Yield ◽  
Fatty Acid Desaturase ◽  
Holstein Cows ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cows ◽  
Fatty Acid Desaturase 2

This study investigated the single nucleotide polymorphisms (SNPs) of Fatty acid desaturase 2 (FADS2) gene and further explored their genetic effects on conventionally collected milk production traits in Chinese Holstein cows using 18,264 test-day records of 841 cows. One missense mutation c. 908 C > T (SNP site in the complementary DNA sequence), which caused an amino acid change from alanine to valine (294Ala > Val), and two 3’ untranslated region (UTR) SNPs, c.1571 G > A and c.2776 A > G were finally identified. The SNP c.908 C > T was significantly associated with test-day milk yield, fat percentage and 305-day milk, fat and protein yield. In particular, the T allele of the SNP c.908 C > T showed a significant association with decreased somatic cell score (SCS) in the investigated population. Significant relationship between the SNP c.1571 G > A and 305-day milk yield showed that genotype GG was linked to the highest milk yield. Substituting the allele G for A at the c.2776 A > G locus resulted in a decrease of protein percentage. Our results demonstrated that FADS2 was an interesting candidate for selection to increase milk production and improve resistance against mastitis.

scholarly journals Genetic Parameters for Milk Somatic Cell Score and Relationships with Production Traits in Primiparous Dairy Sheep

2007 ◽  
Vol 90 (4) ◽  
pp. 1998-2003 ◽  
Author(s):  
V. Riggio ◽  
R. Finocchiaro ◽  
J.B.C.H.M. van Kaam ◽  
B. Portolano ◽  
H. Bovenhuis
Keyword(s):  
Somatic Cell ◽  
Genetic Parameters ◽  
Somatic Cell Score ◽  
Production Traits ◽  
Dairy Sheep
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