Restorative effect of (5E, 13E)–5,13–Docosadienoic acid on carbon tetrachloride induced oxidative stress in rats

The article gives the results of the research on the influence of a developed complex liposomal preparation on the dynamics of morphological and biochemical indicators of the blood of rats subjected to modeled oxidative stress caused by intermuscular injection of 50% carbon tetrachloride at a dose of 0.25 ml per 100 g of body mass. We found that in conditions of intoxication by carbon tetrachloride, the physiological level of hematological indicators of the researched animals’ body was disrupted. This is indicated by the reduction in the amount of erythrocytes, hemoglobin content, concentration of hemoglobin per erythrocyte, increase in the amount of leucocytes, mass of hemoglobin per erythrocyte and increase of colour indicator. Also, we detected suppression of protein synthesis function of the liver. The levels of total protein and albumin fraction were below the normal physiological level. High indicators were observed in the levels of creatinine, urea and total bilirubin. In order to normalize the functional state of the liver under oxidative stress it is reasonable to use a liposomal preparation which contains butafosfan, interferon, thistle and vitamins. In order to normalize morphological and biochemical indicators of the blood of rats subject to intoxication of carbon tetrachloride it is reasonable to use a liposomal preparation that contains butafosfan, selenium, thistle, methionine and vitamins. Using the liposomal preparation Butaselmevit for rats under oxidative stress the morphological and biochemical indicators in the blood normalized. On the 14th day the indictors of the number of erythrocytes, the hemoglobin content, the white blood cell count and red blood indexes compared to control came within the physiological indicators, indicating a recovery of hematopoietic function of the bone marrow and normalized indicators of the functional state of the liver.

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Curative Role of Dithiophenolato Titanium (IV)-Chitosan Nanocomposite Complex Against Carbon Tetrachloride-Induced Liver Injuries

10.21203/rs.3.rs-320823/v1 ◽

2021 ◽

Author(s):

Nadia Z Shaban ◽

Salah A Yehiaa ◽

Doaa Awad ◽

Shaban Y Shaban ◽

Samar R Saleh

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Lipid Profile ◽

Hepg2 Cell ◽

Chitosan Nanoparticles ◽

Antibacterial Activities ◽

Therapeutic Effects ◽

Liver Histopathology ◽

Liver Injuries ◽

Kidney Functions

Abstract Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In our previous studies, dithiophenolato titanium (IV) Complex "DBT" and its chitosan nanocomposite "DBT-CSNPs" were prepared and we showed that these compounds have antibacterial activities, cytotoxic, and have abilities to bind with DNA helixes. Therefore, in this study, we evaluated the LD50 values of dithiophenolato titanium (IV)-complex (DBT) and its high thermal stable chitosan nanoparticles (DBT-CSNPs). Then their therapeutic effects against liver injuries induced by carbon tetrachloride (CCl4) were assessed and compared with cisplatin treatment. Additionally, the anti-proliferative activity of DBT and DBT-CSNPs against human liver cancer (HepG2) cell lines through the analysis of the cell cycle was evaluated. Methods Nine groups of rats were prepared: normal, DBT, DBT-CSNPs, CSNPs, CCl4, CCl4-DBT, CCl4-DBT-CSNPs, CCl4-CSNPs and CCl4-cisplatin. Liver histopathology and the biochemical markers involving oxidative stress, apoptosis, liver and kidney functions, and lipid profile were determined. Results The results revealed that the treatment with DBT-CSNPs and DBT after CCl4 administration abolished liver damage since it reduced the apoptosis induced by CCl4 via the reduction of DNA fragmentation, Bax and caspase- 8 with an elevation of Bcl2 and Bcl2/Bax ratio. Also, these treatments caused nonsignificant changes in the markers of oxidative stress. Therefore, liver histopathology and functions, lipid profile, and kidney functions were improved. Cisplatin treatment reduced liver injury with a degree less than DBT-CSNPs and DBT, but it induced nephrotoxicity. Administration of DBT-CSNPs and DBT to healthy rats for 14 days has no adverse effect. Also, the results showed that DBT-CSNPs and DBT inhibited the proliferation of HepG2 cells by arresting cells in the G2/M phase and inducing cell death. Conclusion DBT-CSNPs and DBT have a therapeutic effect against CCl4-induced liver injuries via the reduction of apoptosis induced by CCl4. Moreover, both compounds have antineoplastic activities against the HepG2 cell line. In all cases, DBT-CSNPs have a greater effect due to their nanostructure. Therefore, both compounds can be used in the pharmacological fields, particularly DBT-CSNPs.

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Protective effect of juice extract of mesocarp rind of Citrullus lanatus in carbon tetrachloride-induced oxidative stress in rats

Journal of Coastal Life Medicine ◽

10.12980/jclm.5.2017j7-61 ◽

2017 ◽

Vol 5 (6) ◽

pp. 264-269

Author(s):

Fisayo Abraham Bamisaye ◽

◽

Abdulhakeem Olarewaju Sulyman ◽

Emmanuel Oladipo Ajani ◽

Aminat Bolanle Muhammed ◽

...

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Protective Effect ◽

Citrullus Lanatus

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Increased Cytotoxicity of Carbon Tetrachloride in a Human Hepatoma Cell Line Overexpressing Cytochrome P450 2E1

Journal of International Medical Research ◽

10.1177/147323000203000406 ◽

2002 ◽

Vol 30 (4) ◽

pp. 400-405 ◽

Cited By ~ 15

Author(s):

S Takahashi ◽

T Takahashi ◽

S Mizobuchi ◽

M Matsumi ◽

K Morita ◽

...

Keyword(s):

Oxidative Stress ◽

Cytochrome P450 ◽

Carbon Tetrachloride ◽

Cell Line ◽

Hepatoma Cell ◽

Mrna Level ◽

Hepatoma Cell Line ◽

Cytochrome P450 2E1 ◽

Hsp70 Mrna ◽

Potential Marker

Cytotoxic free radicals generated during the metabolism of carbon tetrachloride by cytochrome P450 2E1 (CYP2E1) are thought to cause hepatotoxicity. Here, the cytotoxic effects of carbon tetrachloride in a liver cell line expressing CYP2E1 (HLE/2E1) are compared with those in the mother cell line (HLE). The effects of carbon tetrachloride on the gene expression of HSP70, a potential marker of oxidative stress, were also examined. The viability of HLE/2E1 cells after exposure to carbon tetrachloride was significantly decreased compared with that of HLE cells. Northern blot analysis revealed that the HSP70 mRNA level was significantly increased after carbon tetrachloride treatment in both cell lines, while the magnitude of its increase was much greater in HLE/2E1 cells than in HLE cells. These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells.

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Pharmacoprophylaxis of liver diseases: creating a new hepatoprotector

BIO Web of Conferences ◽

10.1051/bioconf/20201700061 ◽

2020 ◽

Vol 17 ◽

pp. 00061

Author(s):

Svetlana Zykova ◽

Sergey Shurov ◽

Aleksey Savinkov ◽

Nino Gugushvili ◽

Vladimir Talismanov

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Toxic Effect ◽

Alanine Aminotransferase ◽

Liver Diseases ◽

Hepatoprotective Activity ◽

Control Group ◽

Biochemical Indicators ◽

Intact Group ◽

Biochemical Studies

The article presents a study of the hepatoprotective activity of a tricyclic heterocycle, which refers to 5, 6, 7, 8-tetrahydroquinolines. The effect of 8, 8-dimethyl-5-p-tolyl-8, 9-dihydro-2H-pyrido [4, 3, 2-de] cinnolin-3 (7H) was studied on rats under the influence of the model of toxic hepatosis induced by carbon tetrachloride to find out the indicators of peroxidation and biochemical indicators. Biochemical studies have shown that modelling toxic fat hepatosis caused by the inception of carbon tetrachloride to rats increased the activity of alanine aminotransferase by 2.5 times more compared with the intact group, indicating the development of oxidative stress induced by the treatment of pyrido [4, 3, 2] Cinnol I that reduced the toxic effect of CTC by 79.9 %. Mexidol had a less pronounced hepatoprotective effect: the activity of Alanine aminotransferase on animals of the second group was lower by 29.2 % than on rats from the control group. Thus, a new compound with hepatoprotective activity has been developed and studied.

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