scholarly journals Prevalence and antibiogram profiles of Escherichia coli O157:H7 isolates recovered from three selected dairy farms in the Eastern Cape Province, South Africa

2016 ◽  
Vol 6 (12) ◽  
pp. 990-995 ◽  
Author(s):  
Luyanda Msolo ◽  
Etinosa Ogbomoede Igbinosa ◽  
Anthony Ifeanyin Okoh
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Dairy Farms ◽  
Escherichia Coli O157 ◽  
Eastern Cape Province ◽  
Eastern Cape

Prevalence and Antimicrobial Susceptibility of Escherichia coli O157:H7 in Vegetables Sold in the Amathole District, Eastern Cape Province of South Africa

2008 ◽  
Vol 71 (4) ◽  
pp. 816-819 ◽  
Author(s):  
B. O. ABONG'O ◽  
M. N. B. MOMBA ◽  
J. N. MWAMBAKANA
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Antimicrobial Susceptibility ◽  
Molecular Techniques ◽  
Escherichia Coli O157 ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
E Coli ◽  
Fresh Vegetables

Fresh vegetables have been implicated in outbreaks of Escherichia coli O157:H7 in most parts of the world. Microbiological quality of vegetables used as recipes for salads is very crucial. Residents of the Amathole District in the Eastern Cape Province of South Africa consume salads frequently, although the microbial quality of recipe vegetables is questionable. The present study investigated the prevalence and antimicrobial susceptibility of E. coli O157:H7 isolated from selected vegetables sold within the Amathole District. One hundred eighty samples of the vegetables were analyzed. Strains of E. coli O157:H7 were isolated by enrichment culture and by immunomagnetic separation and identified by conventional and molecular techniques. In three settlements in this district, the mean counts of presumptive E. coli O157 for the vegetables ranged between 9 × 103 and 1.6 × 106 CFU/g for Fort Beaufort, 1.6 × 103 and 1.6 × 105 CFU/g for Mdantsane, and 1.3 × 103 and 4.1 × 104 CFU/g for Alice. Four (10.3%) of 39 vegetable samples were confirmed to carry E. coli O157:H7. Four representative E. coli O157:H7 isolates from these vegetables were susceptible to at least one of the eight antimicrobial agents tested against them. Even though the prevalence of E. coli O157:H7 was low and those isolated were susceptible to most of the antimicrobials, there remains a need for E. coli O157:H7 surveillance in vegetables used in salad recipes in urban and rural areas of South Africa.

scholarly journals Comparison of 3 tests to detect acaricide resistance in Boophilus decoloratus on dairy farms in the Eastern Cape Province, South Africa

2003 ◽  
Vol 74 (2) ◽  
Author(s):  
S. Mekonnen ◽  
N.R. Bryson ◽  
L.J. Fourie ◽  
R.J. Peter ◽  
A.M. Spickett ◽  
...  
Keyword(s):  
South Africa ◽  
Dairy Farms ◽  
Immersion Test ◽  
Egg Laying ◽  
Eastern Cape Province ◽  
Acaricide Resistance ◽  
Eastern Cape ◽  
Boophilus Decoloratus

The susceptibility of the larval offspring of engorged female Boophilus decoloratus, and of the engorged females, collected from cattle on the dairy farms Brycedale, Sunny Grove and Welgevind in the Eastern Cape Province, South Africa, was tested against the acaricides amitraz, chlorfenvinphos and cypermethrin. Resistance was determined by means of the Shaw Larval Immersion Test (SLIT) for larvae and the Reproductive Estimate Test (RET) and Egg Laying Test (ELT) for adults. At Brycedale the tests all indicated resistance to chlorfenvinphos, and RET and ELT indicated resistance to amitraz and emerging resistance to cypermethrin. At Sunny Grove, B. decoloratus was resistant to cypermethrin using SLIT and exhibited emerging resistance to chlorfenvinphos with SLIT and to cypermethrin with both RET and ELT. At Welgevind, resistance was recorded against chlorfenvinphos (SLIT) and against cypermethrin (ELT), and emerging resistance against permethrin (RET). The results obtained with RET and ELT were generally comparable, but often differed from those obtained with SLIT. Resistance could be detected within 7 days with ELT compared to 42 days with RET and 60 days with SLIT.

scholarly journals Antimicrobial Resistance Profiles of Staphylococcus Aureus Isolated from Meat Carcasses and Bovine Milk in Abattoirs and Dairy Farms of the Eastern Cape, South Africa

2018 ◽  
Author(s):  
Ezekiel Green ◽  
Abongile Pekana
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Bovine Milk ◽  
Dairy Farms ◽  
Penicillin G ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
Biochemical Tests ◽  
Milk Samples ◽  
Antibiotic Susceptibility Test

Background: Staphylococcus aureus (S. aureus) occasionally threatens the life of the host as a persistent pathogen even though it is normal flora of humans and animals. We characterized drug resistance in S. aureus isolated from animal carcasses and milk samples from the abattoirs and dairy farms in the Eastern Cape Province. Methods: A 1000 meat swab samples and 200 raw milk samples were collected from selected abattoirs and dairy farms in the Eastern Cape Province, South Africa. S. aureus was isolated and positively identified using biochemical tests and confirmed by molecular methods. Antibiotic susceptibility test against 14 different antibiotics was performed against all isolates. Antibiotic resistance genes were also detected. Results: Of the 1200 samples collected, 134 (11.2%) samples were positive for S. aureus. Resistance ranged from 71.6% for penicillin G to 39.2% for tetracycline. Resistance gene (blaZ) was detected in 13 (14.9%), while msrA was found in 31 (52.5%) of S. aureus isolates. Conclusions: The present result shows the potential dissemination of multidrug-resistant S. aureus strains in the dairy farms and abattoirs in the Eastern Cape. Therefore, this implies that the organism may rapidly spread through food and pose serious public health risk

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