Biology, immature and adult morphology, and molecular characterization of a new species of the genusEntedon(Hymenoptera: Eulophidae) associated with the invasive pestSpecularius impressithorax(Coleoptera: Chrysomelidae, Bruchinae) onErythrinaplants

2011 ◽  
Vol 101 (6) ◽  
pp. 715-739 ◽  
Author(s):  
A.V. Gumovsky ◽  
M.M. Ramadan
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Early Embryo ◽  
Invasive Pest ◽  
Young Larva ◽  
Preimaginal Stages ◽  
Undifferentiated Cells ◽  
Palearctic Species ◽  
Coral Tree

AbstractEntedon erythrinaesp. n. (Hymenoptera: Eulophidae), a gregarious egg-larval endoparasitoid of the Erythrina bruchineSpecularius impressithorax, an invasive pest of the coral tree seeds (Erythrinaspp.), is described from the Hawaiian Islands and Africa (South Africa, Tanzania and Mozambique). The biology and morphology of preimaginal stages of this new species are described in details.It is remarkable that the early embryo of the parasitoid represents a mass of undifferentiated cells surrounded by a peculiar membrane formed by the peripheral enlarged polygonal cells. The young larva developing inside this membrane corresponds morphologically to the second instar of congeneric species. Various peculiarities of the parasitoid-host relationships in gregarious and solitaryEntedonparasitoids are discussed. The DNA sequences of 28S D2 (nuclear), Cytochrome Oxidase I (COI, mitochondrial) and Cytochrome B (CytB, mitochondrial) genes are provided for this new species and compared with the sequences of some other Afrotropical and Palearctic species of the genus.

scholarly journals Revision of the Western Palearctic Meteorini (Hymenoptera, Braconidae), with a molecular characterization of hidden Fennoscandian species diversity

Zootaxa ◽  
2011 ◽  
Vol 3084 (1) ◽  
pp. 1 ◽  
Author(s):  
JULIA STIGENBERG ◽  
FREDRIK RONQUIST
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Cryptic Species ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
New Synonyms ◽  
Western Palearctic ◽  
Palearctic Species ◽  
Traditional Approaches

The tribe Meteorini includes two genera, Meteorus and Zele, which are koinobiont endoparasitoids of larval Lepidoptera and Coleoptera. More than 300 species are known, about one fifth of which occur in the Western Palearctic. Here, we revise the Western Palearctic species, based partly on traditional approaches and partly on molecular analysis of recent Swedish and Finnish material. For the analyses of phylogenetic relationships and cryptic species diversity, we coded 17 morphological characters and sequenced two markers, 28S D2 (649 bp) and CO1 (665 bp). More than 1 970 specimens representing 54 species of Meteorus Haliday and 5 species of Zele Curtis were studied; of these, 177 specimens representing 41 species were sequenced. Seven new species are described, all from the Fennoscandian material: Meteorus artocercus sp. nov., M. densipilosus sp. nov., M. eklundi sp. nov., M. longipilosus sp. nov., M. sibyllae sp. nov., M. stenomastax sp. nov., and M. subtilisulcus sp. nov. Four new synonyms are introduced: Z. chlorophthalmus (Spinola 1808), syn. nov. for M. pallidus (Nees 1812), M. punctifrons Thomson 1895, syn. nov. for M. varinervis (Tobias 1986), M. melanostictus Capron 1887, syn. nov for M. monachae (Tobias 1986), and M. tenellus Marshall 1887, syn. nov. for M. boreus (Tobias 1986). Meteorus tenellus is removed from synonymy with M. cinctellus. Sequence analysis indicated the presence of at least 12 additional cryptic species but these cannot be separated morphologically at this point and, therefore, we do not describe them here. The phylogenetic results suggest that Zele should be included within Meteorus but we refrain from formal changes of the generic classification until more comprehensive phylogenetic analyses of the tribe can be completed. A key to the known Western Palearctic species is presented.

scholarly journals Morphological and molecular characterization of Langdonia walkerae sp. nov. infecting Aristida stricta and A. beyrichiana in longleaf pine-grassland ecosystems in the southeastern USA

2021 ◽  
Author(s):  
A.S. Alqurashi ◽  
J. Kerrigan ◽  
K.G. Savchenko
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Longleaf Pine ◽  
Spore Wall ◽  
Southeastern Usa ◽  
Grassland Ecosystems ◽  
Spore Wall Ornamentation ◽  
A New Species

A smut fungus that hinders wiregrass restoration efforts in longleaf pine-grassland ecosystems was collected from Aristida stricta and A. beyrichiana (Poaceae) in three states in the southeastern USA. Morphological and phylogenetic characteristics of this fungus were examined. These data show that the specimens from both plant species were infected by the same fungus and represent a new species of Langdonia. The new species differs morphologically from other species of Langdonia by teliospores being solitary and not compacted into spore balls. Spore wall ornamentation and teliospore size also differ from other Langdonia species. Phylogenetic analyses of DNA sequences of the ITS, LSU, and EF-1α supported separation of the species from A. stricta and A. beyrichiana from other Langdonia species. Based on these results, a new species, Langdonia walkerae, is proposed.

A new section of Silene (Caryophyllaceae) including a new species from South Anatolia, Turkey 

Phytotaxa ◽  
2014 ◽  
Vol 178 (2) ◽  
pp. 98 ◽  
Author(s):  
Zeynep Aydin ◽  
Alaattin Selçuk Ertekin ◽  
Elisabeth Långström ◽  
Bengt Oxelman
Keyword(s):  
New Species ◽  
New Taxa ◽  
Dna Sequences ◽  
The Other ◽  
Diagnostic Characters ◽  
Nuclear Its ◽  
A New Species

Silene section Cryptoneurae (Caryophyllaceae) and S. ertekinii from Western Antalya, Turkey, are described as new taxa on the basis of morphological and molecular investigations. Nuclear ITS and chloroplast rps16 DNA sequences clearly support the recognition of the new section. Inclusiveness and characterization of the new section is discussed and described, and a key for the included species is provided. Diagnostic characters of the new species S. ertekinii are given and its relation to the other species of the section is discussed.

scholarly journals Nothing in (sponge) biology makes sense – except when based on holotypes

2015 ◽  
Vol 96 (2) ◽  
pp. 305-311 ◽  
Author(s):  
Dirk Erpenbeck ◽  
Merrick Ekins ◽  
Nicole Enghuber ◽  
John N.A. Hooper ◽  
Helmut Lehnert ◽  
...  
Keyword(s):  
New Species ◽  
Reference Material ◽  
Species Identification ◽  
Dna Sequences ◽  
Morphological Plasticity ◽  
Morphological Characters ◽  
Molecular Techniques ◽  
Reference Sequences ◽  
Species Descriptions ◽  
Preservation History

Sponge species are infamously difficult to identify for non-experts due to their high morphological plasticity and the paucity of informative morphological characters. The use of molecular techniques certainly helps with species identification, but unfortunately it requires prior reference sequences. Holotypes constitute the best reference material for species identification, however their usage in molecular systematics and taxonomy is scarce and frequently not even attempted, mostly due to their antiquity and preservation history. Here we provide case studies in which we demonstrate the importance of using holotype material to answer phylogenetic and taxonomic questions. We also demonstrate the possibility of sequencing DNA fragments out of century-old holotypes. Furthermore we propose the deposition of DNA sequences in conjunction with new species descriptions.

Cloning and characterization of DNA sequences amplified in multidrug-resistant Djungarian hamster and mouse cells

1987 ◽  
Vol 13 (6) ◽  
pp. 609-619 ◽  
Author(s):  
A. V. Gudkov ◽  
O. B. Chernova ◽  
A. R. Kazarov ◽  
B. P. Kopnin
Keyword(s):  
Dna Sequences ◽  
Multidrug Resistant ◽  
Djungarian Hamster ◽  
Mouse Cells
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