Cloning and characterization of DNA sequences amplified in multidrug-resistant Djungarian hamster and mouse cells

1987 ◽  
Vol 13 (6) ◽  
pp. 609-619 ◽  
Author(s):  
A. V. Gudkov ◽  
O. B. Chernova ◽  
A. R. Kazarov ◽  
B. P. Kopnin
Keyword(s):  
Dna Sequences ◽  
Multidrug Resistant ◽  
Djungarian Hamster ◽  
Mouse Cells

scholarly journals Isolation and characterization of DNA sequences amplified in multidrug-resistant hamster cells.

1986 ◽  
Vol 83 (2) ◽  
pp. 337-341 ◽  
Author(s):  
P. Gros ◽  
J. Croop ◽  
I. Roninson ◽  
A. Varshavsky ◽  
D. E. Housman
Keyword(s):  
Dna Sequences ◽  
Multidrug Resistant ◽  
Isolation And Characterization

scholarly journals Molecular characterization of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from King Abdulaziz Specialist Hospital at Taif City, Saudi Arabia

2021 ◽  
Vol 14 (1) ◽  
pp. 143-151
Author(s):  
Rihab Lagha ◽  
Fethi Ben Abdallah ◽  
Asmaa A.H. ALKhammash ◽  
Nabil Amor ◽  
Mohamed M. Hassan ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Multidrug Resistant ◽  
Clinical Isolates

scholarly journals S. pseudintermedius and S. aureus lineages with transmission ability circulate as causative agents of infections in pets for years

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Laura Ruiz-Ripa ◽  
Carmen Simón ◽  
Sara Ceballos ◽  
Carmelo Ortega ◽  
Myriam Zarazaga ◽  
...  
Keyword(s):  
Treatment Options ◽  
Companion Animals ◽  
Clinical History ◽  
Multidrug Resistant ◽  
Hospital Environment ◽  
Antimicrobial Resistance Profile ◽  
Causative Agents ◽  
Veterinary Hospital ◽  
Coagulase Positive Staphylococci

Abstract Background Staphylococcus pseudintermedius (SP) and Staphylococcus aureus (SA) are common colonizers of companion animals, but they are also considered opportunistic pathogens, causing diseases of diverse severity. This study focused on the identification and characterization of 33 coagulase-positive staphylococci isolated from diseased pets (28 dogs and five cats) during 2009–2011 in a veterinary hospital in Spain in order to stablish the circulating lineages and their antimicrobial resistance profile. Results Twenty-eight isolates were identified as SP and five as SA. Nine methicillin-resistant (MR) isolates (27%) carrying the mecA gene were detected (eight MRSP and one MRSA). The 55% of SP and SA isolates were multidrug-resistant (MDR). MRSP strains were typed as ST71-agrIII-SCCmecII/III-(PFGE) A (n=5), ST68-agrIV-SCCmecV-B1/B2 (n=2), and ST258-agrII-SCCmecIV-C (n=1). SP isolates showed resistance to the following antimicrobials [percentage of resistant isolates/resistance genes]: penicillin [82/blaZ], oxacillin [29/mecA] erythromycin/clindamycin [43/erm(B)], aminoglycosides [18–46/aacA-aphD, aphA3, aadE], tetracycline [71/tet(M), tet(K)], ciprofloxacin [29], chloramphenicol [29/catpC221], and trimethoprim-sulfamethoxazole [50/dfrG, dfrK]. The dfrK gene was revealed as part of the radC-integrated Tn559 in two SP isolates. Virulence genes detected among SP isolates were as follow [percentage of isolates]: siet [100], se-int [100], lukS/F-I [100], seccanine [7], and expB [7]. The single MRSA-mecA detected was typed as t011-ST398/CC398-agrI-SCCmecV and was MDR. The methicillin-susceptible SA isolates were typed as t045-ST5/CC5 (n=2), t10576-ST1660 (n=1), and t005-ST22/CC22 (n=1); the t005-ST22 feline isolate was PVL-positive and the two t045-ST45 isolates were ascribed to Immune Evasion Cluster (IEC) type F. Moreover, the t10576-ST1660 isolate, of potential equine origin, harbored the lukPQ and scneq genes. According to animal clinical history and data records, several strains seem to have been acquired from different sources of the hospital environment, while some SA strains appeared to have a human origin. Conclusions The frequent detection of MR and MDR isolates among clinical SP and SA strains with noticeable virulence traits is of veterinary concern, implying limited treatment options available. This is the first description of MRSA-ST398 and MRSP-ST68 in pets in Spain, as well the first report of the dfrK-carrying Tn559 in SP. This evidences that current transmissible lineages with mobilizable resistomes have been circulating as causative agents of infections among pets for years.

scholarly journals Genome characterization of ESBL-producing multidrug-resistant Escherichia coli in Rabat, Morocco

2021 ◽  
Author(s):  
Jérôme Ambroise ◽  
Elmostafa Benaissa ◽  
Léonid M. Irenge ◽  
EL Mehdi Belouad ◽  
Bertrand Bearzatto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Genome Characterization

Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil

2021 ◽  
Author(s):  
Rafael Nakamura-Silva ◽  
Mariana Oliveira-Silva ◽  
João Pedro Rueda Furlan ◽  
Eliana Guedes Stehling ◽  
Carlos Eduardo Saraiva Miranda ◽  
...  
Keyword(s):  
High Risk ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Northeastern Brazil ◽  
Clonal Group

scholarly journals Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

2021 ◽  
Vol 9 (2) ◽  
pp. 423
Author(s):  
Ahmed Esmael ◽  
Ehab Azab ◽  
Adil A. Gobouri ◽  
Mohamed A. Nasr-Eldin ◽  
Mahmoud M. A. Moustafa ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Multidrug Resistant ◽  
Viable Count ◽  
Food Storage ◽  
Chicken Breast ◽  
Whole Genome Analysis ◽  
Isolation And Characterization ◽  
Phage Cocktail ◽  
Global Threat

Foodborne salmonellosis is a global threat to public health. In the current study, we describe the isolation and characterization of two broad-spectrum, lytic Salmonella phages: SPHG1 and SPHG3 infecting a multidrug-resistant Salmonella Typhimurium EG.SmT3. Electron microscopy and whole genome analysis identified SPHG1 as a Myovirus, while SPHG3 as a new member of the genus “Kuttervirus” within the family Ackermannviridae. SPHG1 and SPHG3 had a lysis time of 60 min. with burst sizes of 104 and 138 PFU/cell, respectively. The two phages were robust at variable temperatures and pH ranges that match the corresponding values of most of the food storage and processing conditions. A phage cocktail containing the two phages was stable in the tested food articles for up to 48 h. The application of the phage cocktail at MOIs of 1000 or 100 resulted in a significant reduction in the viable count of S. Typhimurium by 4.2 log10/sample in milk, water, and on chicken breast. Additionally, the phage cocktail showed a prospective ability to eradicate and reduce the biofilm that formed by S. Typhimurium EG.SmT3. A phage cocktail of SPHG1 and SPHG3 is considered as a promising candidate as a biocontrol agent against foodborne salmonellosis due to its broad host ranges, highly lytic activities, and the absence of any virulence or lysogeny-related genes in their genomes.

scholarly journals Characterization of Two Multidrug-Resistant IncA/C Plasmids from the 1960s by Using the MinION Sequencer Device

2016 ◽  
Vol 60 (11) ◽  
pp. 6780-6786 ◽  
Author(s):  
Mónika Szabó ◽  
Tibor Nagy ◽  
Tímea Wilk ◽  
Tibor Farkas ◽  
Anna Hegyi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Genome ◽  
Multidrug Resistant ◽  
Phenotypic Change ◽  
Long Reads ◽  
Loss Of Resistance ◽  
Rapid Characterization ◽  
Resistance Island ◽  
The 1960S

ABSTRACTTwo A/C incompatibility group (IncA/C family) plasmids from the 1960s have been sequenced and classified into the A/C2type 1 group. R16a and IP40a contain novel antibiotic resistance islands and a complete GIsul2 genomic island not previously found in the family. In the 173.1-kb R16a, the 29.9-kb antibiotic resistance island (ARI) is located in a unique backbone position not utilized by ARIs. ARIR16aconsists of Tn1, Tn6020, and Tn6333, harboring the resistance genesblaTEM-1DandaphA1band amermodule, respectively; a truncated Tn5393copy; and a gene cluster with unknown function. Plasmid IP40a is 170.4 kb in size and contains a 5.6-kb ARI inserted into thekfrAgene. ARIIP40acarryingblaTEM-1DandaphA1bgenes is composed of Tn1with a Tn6023insertion. Additionally, IP40a harbors single IS2, IS186, and Tn1000insertions scattered in the backbone; an IS150copy in GIsul2; and a complete Tn6333carrying amermodule at the position of ARIR16a. Loss of resistance markers in R16a, IP40a, and R55 was observed during stability tests. Every phenotypic change proved to be the result of recombination events involving mobile elements. Intramolecular transposition of IS copies that generated IP40a derivatives lacking large parts of the backbone could account for the formation of other family members, too. The MinION platform proved to be a valuable tool in bacterial genome sequencing since it generates long reads that span repetitive elements and facilitates full-length plasmid or chromosome assembly. Nanopore technology enables rapid characterization of large, low-copy-number plasmids and their rearrangement products.

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