A simple molecular identification method of the Thrips tabaci (Thysanoptera: Thripidae) cryptic species complex

AbstractThe onion thrips (Thrips tabaci Lindeman, 1889) is a key pest of a wide range of crops because of its ecological attributes such as polyphagy, high reproduction rate, ability to transmit tospoviruses and resistance to insecticides. Recent studies revealed that T. tabaci is a cryptic species complex and it has three lineages (leek-associated arrhenotokous L1-biotype, leek-associated thelytokous L2-biotype and tobacco-associated arrhenotokous T-biotype), however, the adults remain indistinguishable. T. tabaci individuals were collected from different locations of Hungary to create laboratory colonies from each biotypes. Mitochondrial COI (mtCOI) region was sequenced from morphologically identified individuals. After sequence analysis SNPs were identified and used for CAPS marker development, which were suitable for distinguishing the three T. tabaci lineages. Genetic analysis of the T. tabaci species complex based on mtCOI gene confirmed the three well-known biotypes (L1, L2, T) and a new biotype because the new molecular evidence presented in this study suggests T-biotype of T. tabaci forming two distinct (sub)clades (T1 and T2). This genetic finding indicates that the genetic variability of T. tabaci populations is still not fully mapped. We validated our developed marker on thrips individuals from our thrips colonies. The results demonstrated that the new marker effectively identifies the different T. tabaci biotypes. We believe that our reliable genotyping method will be useful in further studies focusing on T. tabaci biotypes and in pest management by scanning the composition of sympatric T. tabaci populations.

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Hidden Mediterranean biodiversity: molecular evidence for a cryptic species complex within the reef building vermetid gastropod Dendropoma petraeum (Mollusca: Caenogastropoda)

Biological Journal of the Linnean Society ◽

10.1111/j.1095-8312.2008.01167.x ◽

2009 ◽

Vol 96 (4) ◽

pp. 898-912 ◽

Cited By ~ 59

Author(s):

MARTA CALVO ◽

JOSÉ TEMPLADO ◽

MARCO OLIVERIO ◽

ANNIE MACHORDOM

Keyword(s):

Cryptic Species ◽

Species Complex ◽

Molecular Evidence ◽

Cryptic Species Complex ◽

Mediterranean Biodiversity

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Climatic niche comparison across a cryptic species complex

PeerJ ◽

10.7717/peerj.7042 ◽

2019 ◽

Vol 7 ◽

pp. e7042 ◽

Cited By ~ 4

Author(s):

Qing Zhao ◽

Hufang Zhang ◽

Jiufeng Wei

Keyword(s):

Cryptic Species ◽

Species Complex ◽

Potential Distribution ◽

Niche Overlap ◽

Climatic Variables ◽

Distribution Model ◽

Molecular Evidence ◽

Climatic Niche ◽

Distribution Maps ◽

Cryptic Species Complex

According to current molecular evidence, the Chionaspis pinifoliae heterophyllae species complex has been recognized as 10 cryptic species. In this study, we construct potential distribution maps for seven cryptic species based on climatic variables. This was done to assess the main environmental factors that have contributed to the distribution map and test the degree of niche overlap across the seven cryptic species. We used MaxEnt to build the climatic niche models under climatic variables. For these models, the similarities and differences of the niches across the cryptic species were estimated. By comparing the potential distribution model of each cryptic species, our results suggested parapatric, sympatric and allopatry populations for this cryptic species complex. Our results showed high variability in niche overlap, and more often niche conservatism than niche divergence. The current species delimitation of the Chionaspis pinifoliae heterophyllae complex by molecular information and the hypothesis that the niche overlap in the sympatric population is higher than that of the allopatry population were supported based on the findings. This study will provide baseline data and a distribution range to facilitate the further control of these insects and formulate quarantine measures.

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